Abstract
The enzyme terminal deoxynucleotidyl transferase (TdT) is expressed on the nuclear membrane of normal precursor B and T cells as well as their malignant counterparts, i.e., acute lymphoblastic leukemias (ALLs) and some lymphoblastic lymphomas [1, 2]. TdT expression has also been found in 5%–46% of acute nonlymphocytic leukemias (ANLLs) [3–9]. In ANLL there is a large variability in the percentage of TdT+ cells, and also the intensity of TdT expression varies per cell. In most studies a limit of at least 10% of TdT+ cells was adopted for the diagnosis of a TdT+ ANLL. However, it is likely that in some ANLLs smaller TdT+ leukemic subpopulations are present.
This work was supported by the Sophia Foundation for Medical Research
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bollum FJ (1979) Terminal deoxynucleotidyl transferase as a hematopoietic cell marker. Blood 54:1203–1215
Janossy G, Hoffbrand AV, Greaves MF, Ganeshaguru K, Pain C, Bradstock KF, Prentice HG, Kay HEM, Lister TA (1980) Terminal transferase enzyme assay and immunological membrane markers in the diagnosis of leukaemia:a multiparameter analysis of 300 cases. Br J Haematol 44:221–234
Bradstock KF, Hoffbrand AV, Ganeshaguru K, Llewellin P, Patterson K, Wonke B, Prentice AG, Bennett M, Pizzolo G, Bollum FJ, Janossy G (1981) Terminal deoxynucleotidyl transferase expression in acute non-lymphoid leukaemia:an analysis by immunofluorescence. Br J Haematol 47:133–143
Jani P, Verbi W, Greaves MF, Bevan D, Bollum F (1983) Terminal deoxynucleotidyl transferase in acute myeloid leukaemia. Leuk Res 7:17–29
Lanham GR, Melvin SL, Stass SA (1985) Immunoperoxidase determination of terminal deoxynucleotidyl transferase in acute leukemia using PAP and ABC methods:experience in 102 cases. Am J Clin Pathol 83:366–370
Krause JR, Brody JP, Kaplan SS, Penchansky L (1986) Terminal deoxynucleotidyl transferase activity in acute leukemia:a study of 100 cases comparing an immunoperoxidase (PAP) vs. immunofluorescent method. Am J Hematol 22:179–184
Kaplan SS, Penchansky L, Krause JR, Basford RE, Zdziarski U (1987) Simultaneous evaluation of terminal deoxynucleotidyl transferase and myeloperoxidase in acute leukemias using an immunocytochemical method. Am J Clin Pathol 87:732–738
Erber WN, Mason DY (1987) Immunoalka- line phosphatase labeling of terminal transferase in hematologic samples. Am J Clin Pathol 88:43–50
Parreira A, Pombo de Oliveira MS, Matutes E, Foroni L, Morilla R, Catovsky D (1988) Terminal deoxynucleotidyl transferase positive acute myeloid leukaemia:an association with immature myeloblasts leukaemia. Br J Haematol 69:219–224
Van Dongen JJM, Adriaansen HJ, Hooijkaas H (1987) Immunological marker analysis of cells in the various hematopoietic differentiation stages and their malignant counterparts. In:Ruiter DJ, Fleuren GJ, Warnaar SO (eds) Application of monoclonal antibodies in tumor pathology. Nijhoff, Dordrecht, pp 87–116
Adriaansen HJ, Van Dongen JJM, Kappers- Klunne MC, Hählen K, Van’t Veer MB, Wijdenes-de Bresser JHFM, Holdrinet ACJM, Harthoorn-Lasthuizen EJ, Abels J, Hooijkaas H (submitted) Terminal deoxynucleotidyl transferase positive subpopulations occur in the majority of ANLL. Implications for the detection of minimal disease
Janossy G, Bollum FJ, Bradstock KF, McMichael A, Rapson N, Greaves MF (1979) Terminal transferase-positive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia. J Immunol 123:1525–1529
Bradstock KF, Kerr A, Kabral A, Favaloro EJ, Hewson JW (1986) Coexpression of pi 65 myeloid surface antigen and terminal de-oxynucleotidyl transferase:a comparison of acute myeloid leukaemia and normal bone marrow cells. Am J Hematol 23:43–50
Sobol RE, Mick R, Royston I, Davey FR, Ellison RR, Newman R, Cuttner J, Griffin JD, Collins H, Nelson DA, Bloomfield CD (1987) Clinical importance of myeloid antigen expression in adult acute lymphoblastic leukemia. N Engl J Med 316:1111–1117
Desiderio SV, Yancopoulos GD, Paskind M, Thomas E, Boss MA, Landau N, Alt FW, Baltimore D (1984) Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells. Nature 311:752–755
Landau NR, Schatz DG, Rosa M, Baltimore D (1987) Increased frequency of N-region insertion in a murine pre-B-cell line infected with a terminal deoxynucleotidyl transferase retroviral expression vector. Mol Cell Biol 7:3237–3243
Van Dongen JJM, Hooijkaas H, Adriaansen HJ, Hählen K, Van Zanen GE (1986) Detection of minimal residual acute lymphoblastic leukemia by immunological marker analysis:possibilities and limitations. In:Hagenbeek A, Lowenberg B (eds) Minimal residual disease in acute leukemia 1986. Nijhoff, Dordrecht, pp 113–133
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Adriaansen, H.J., Hooijkaas, H., Kappers-Klunne, M.C., Hählen, K., van’t Veer, M.B., van Dongen, J.J.M. (1990). Double Marker Analysis for Terminal Deoxynucleotidyl Transferase and Myeloid Antigens in Acute Nonlymphocytic Leukemia Patients and Healthy Subjects. In: Büchner, T., Schellong, G., Hiddemann, W., Ritter, J. (eds) Acute Leukemias II. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 33. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74643-7_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-74643-7_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-50984-4
Online ISBN: 978-3-642-74643-7
eBook Packages: Springer Book Archive