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Ultraviolet Microscopy

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Methods in Lignin Chemistry

Part of the book series: Springer Series in Wood Science ((SSWOO))

Abstract

Lignin in wood absorbs ultraviolet light much more strongly than the polysaccharide components. It is not surprising, therefore, that many authors have used UV microscopy to study lignin in wood. Lange (1954) determined the lignin content in the middle lamella and the secondary wall of spruce tracheids from UV photomicrographs. Wardrop (1957) utilized UV photomicrographs for detecting the beginning of lignification and its subsequent progress in differentiating xylem. Both Lange (1954) and Wergin (1965) elucidated the unusual lignin distribution in compression wood by means of UV photomicrographs. Jayme and Torgersen (1967) reported on the topochemistry of delignification in sulfite and sulfate pulping of spruce wood using a Leitz UV microscope.

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References

  • Aulin-Erdtman G (1954) Spectrographs contributions to lignin chemistry V. Sven Papperstidn 57: 745–760

    CAS  Google Scholar 

  • Boutelje JB, Eriksson I (1982) An UV-microscopy study of lignin in middle lamella fragments from fibers of mechanical pulp of spruce. Sven Papperstidn 85: R39–42

    CAS  Google Scholar 

  • Boutelje JB, Eriksson I (1984) Analysis of lignin in fragments from thermomechanical spruce pulp by ultraviolet microscopy. Holzforschung 38: 249–252

    Article  CAS  Google Scholar 

  • Boutelje JB, Jonsson U (1980) Ultraviolet microscope photometry of pulp fibers. Cellul Chem Technol 14: 53–67

    CAS  Google Scholar 

  • Eastman Kodak Co. (1987) Scientific imaging with Kodak films and plates. Publ No. P-315, Rochester, New York

    Google Scholar 

  • Fergus BJ, Goring DAI (1969) The topochemistry of delignification in kraft and neutral sulphite pulping of birch wood. Pulp Pap Mag Can 70:T-314

    Google Scholar 

  • Fergus BJ, Goring DAI (1970a) The location of guaiacyl and syringyl lignins in birch xylem tissue. Holzforschung 24: 113–117

    Article  CAS  Google Scholar 

  • Fergus BJ, Goring DAI (1970b) The distribution of lignin in birch wood as determined by ultraviolet microscopy. Holzforschung 24: 118–124

    Article  CAS  Google Scholar 

  • Fergus BJ, Procter AR, Scott JAN, Goring DAI (1969) The distribution of lignin in sprucewood as determined by ultraviolet microscopy. Wood Sci Technol 3: 117–138

    Article  Google Scholar 

  • Fujii T (1987) Ultraviolet microspectophotometry of MWL embedded with epxyresin. Proc 32nd Jpn Lignin Symp, Fukuoka, Japan, 21–24

    Google Scholar 

  • Fujii T, Shimizu K, Yamaguchi A (1987) Enzymatic saccharification on ultrathin sections and ultraviolet spectra of Japanese hardwood and softwoods. Mokuzai Gakkaishi 33: 400–407

    Google Scholar 

  • Fukazawa K (1974) The distribution of lignin in compression- and lateral-wood of Abies sachalinensis using ultraviolet microscopy. Res Bull Coll Exp For Hokkaido Univ 31: 87–114

    Google Scholar 

  • Fukazawa K, Imagawa H (1981) Quantitative analysis of lignin using an UV microscopic image analyzer. Variation within one growth increment. Wood Sci Technol 15: 45–55

    Article  CAS  Google Scholar 

  • Fukazawa K, Imagawa H (1983) Ultraviolet and fluorescence microscopic studies of lignin. 1983 Proc Int Symp Wood Pulp Chem May 23-27, 1983, Tsukuba, Japan, TAPPI 1: 20–23

    Google Scholar 

  • Imagawa H (1981) Study on the seasonal development of the secondary phloem in Larix leptolepis. Res Bull Coll Exp For Hokkaido Univ 38: 31–44

    Google Scholar 

  • Imagawa H, Fukazawa K (1978) Influence of wood extractives on UV microscopy for lignin determination. Mokuzai Gakkaishi 24: 583–586

    CAS  Google Scholar 

  • Imagawa H, Fukazawa K, Ishida S (1976) Study on the lignification in tracheids of Japanese larch, Larix leptolepis Gord. Res Bull Coll Exp For Hokkaido Univ 33: 127–138

    Google Scholar 

  • Jayme G, Torgersen HF (1967) Topochemie der Delignifizierung beim Aufschluss von Fichtenholz nach dem Sulfit- und Sulfatverfahren Teil 1 Ultraviolett-mikroskopische Untersuchungen an teilweise delignifiziertem Fichtenholz. Holzforschung 21: 110–116

    Article  CAS  Google Scholar 

  • Kojima Y, Yoon SY, Kayama T (1988a) Delignification from the cell wall of wood fibers with ozone. Mokuzai Gakkaishi 34: 697–701

    CAS  Google Scholar 

  • Kojima Y, Yoon SY, Kayama T (1988b) A study of production of CTMP from hardwood. Part 3. Characterization of pulps produced by CTMP-03 process. J Jpn Tappi 42: 953–962

    Article  CAS  Google Scholar 

  • Lange PW (1954) The distribution of lignin in the cell wall of normal and reaction wood from spruce and a few hardwoods. Sven Papperstidn 57: 525–532

    CAS  Google Scholar 

  • Luft JH (1961) Improvements in epoxy resin embedding method. J Biophys Biochem Cytol 9: 409–414

    Article  PubMed  CAS  Google Scholar 

  • Musha Y, Goring DAI (1975a) Cell dimensions and their relationship to the chemical nature of the lignin from the wood of broad-leaved trees. Can J For Res 5: 259–268

    Article  Google Scholar 

  • Musha Y, Goring DAI (1975b) Distribution of syringyl and guaiacyl moieties in hardwoods as indicated by ultraviolet microscopy. Wood Sci Technol 9: 45–58

    Article  CAS  Google Scholar 

  • Procter AR, Yean WO, Goring DAI (1967) The topochemistry of delignification in kraft and sulphite pulping of spruce wood. Pulp Pap Mag Can 68: T45–T53

    Google Scholar 

  • Saka S, Whiting P, Fukazawa K, Goring DAI (1982) Comparative studies on lignin distribution by UV microscopy and bromination combined with EDXA. Wood Sci Technol 16: 269–277

    Article  CAS  Google Scholar 

  • Scott JAN, Goring DAI (1970a) Lignin concentration in the S3 layer of softwoods. Cellul Chem Technol 4: 83–93

    CAS  Google Scholar 

  • Scott JAN, Goring DAI (1970b) Photolysis of wood microsections in the ultraviolet microscopy. Wood Sci Technol 4: 237–239

    Article  Google Scholar 

  • Scott JAN, Procter AR, Fergus BJ, Goring DAI (1969) The application of ultraviolet microscopy to the distribution of lignin in wood. Description and validity of the technique. Wood Sci Technol 3: 73–92

    Article  Google Scholar 

  • Takabe K, Fukazawa K, Terashima N, Fukushima K (1987) Lignification process of hardwoods xylem elements by UV microspectrophotometry. Proc 32nd Jpn Lignin Symp, Fukuoka, Japan, 1–4

    Google Scholar 

  • Takano T, Fukazawa K, Ishida S (1983) Within-a-ring variation of lignin in Picea glehnii by UV microscopic image analysis. Res Bull Coll Exp For Hokkaido Univ 40: 709–722

    CAS  Google Scholar 

  • Wardrop AB (1957) The phase of lignification in the differentiation of wood fibers. Tappi 40: 225

    CAS  Google Scholar 

  • Wergin W (1965) Ãœber Entstehung und Aufbau von Reaktionsholzzellen. 4. Mitt.: Nachweise der Ligninverteilung in den Zellwänden des Druckholzes durch Untersuchungen im UV- Licht. Flora 156: 322–331

    Google Scholar 

  • Wood JR, Ahlgren PA, Goring DAI (1972) Topochemistry in the chlorite delignification of spruce wood. Sven Papperstidn 15: 15–19

    Google Scholar 

  • Wood JR, Goring DAI (1971) The distribution of lignin in stem wood and branch wood of Douglas fir. Pulp Pap Mag Can 72: T95–T102

    CAS  Google Scholar 

  • Wood JR, Goring DAI (1973) Ultraviolet microscopy at wavelengths below 240nm. J Microscopy 100: 105–111

    Article  Google Scholar 

  • Yang JM, Goring DAI (1978a) A comparison of the concentration of free phenolic hydroxyl groups in the secondary wall and middle lamella regions of softwoods. Trans Tech Sect CPPA 4: 2–5

    Google Scholar 

  • Yang JM, Goring DAI (1978b) The topochemistry of the reaction of chlorine dioxide with lignin in black spruce wood. Holzforschung 32: 185–188

    Article  CAS  Google Scholar 

  • Yang JM, Goring DAI (1980) The phenolic hydroxyl content of lignin in spruce wood. Can J Chem 58: 2411–2414

    Article  CAS  Google Scholar 

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© 1992 Springer-Verlag Berlin Heidelberg

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Fukazawa, K. (1992). Ultraviolet Microscopy. In: Lin, S.Y., Dence, C.W. (eds) Methods in Lignin Chemistry. Springer Series in Wood Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74065-7_8

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  • DOI: https://doi.org/10.1007/978-3-642-74065-7_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74067-1

  • Online ISBN: 978-3-642-74065-7

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