High Performance Liquid Chromatography (HPLC)

  • N. G. Lewis
Part of the Springer Series in Wood Science book series (SSWOO)


The general principles of liquid chromatography were first outlined in 1903 by the botanist Tswett in his description of adsorption chromatography for the separation of natural pigments, such as chlorophylls (Tswett 1903). Since then remarkable progress has been made, as evidenced by the multitude of techniques employed today in thin layer, low pressure, flash, and high performance liquid chromatographic (HPLC) methods (Heftmann 1983). Without these developments, the separation and isolation of chemical constituents within complex, and even simple, mixtures would not be possible. To underscore the general importance of chromatography, two examples will suffice: our knowledge of mechanisms in organic chemistry (including that pertaining to lignin) would be very limited if products could not be isolated and quantified by chromatographic methods, and their structures subsequently determined. Similarly, without chromatography, our detailed understanding of biochemical pathways and metabolic products would not be as highly developed as it is today.


High Performance Liquid Chromatography Hydroxycinnamic Acid Retention Volume Mobile Phase Composition Reversed Phase Chromatography 
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© Springer-Verlag Berlin Heidelberg 1992

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  • N. G. Lewis

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