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High Performance Liquid Chromatography (HPLC)

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Methods in Lignin Chemistry

Part of the book series: Springer Series in Wood Science ((SSWOO))

Abstract

The general principles of liquid chromatography were first outlined in 1903 by the botanist Tswett in his description of adsorption chromatography for the separation of natural pigments, such as chlorophylls (Tswett 1903). Since then remarkable progress has been made, as evidenced by the multitude of techniques employed today in thin layer, low pressure, flash, and high performance liquid chromatographic (HPLC) methods (Heftmann 1983). Without these developments, the separation and isolation of chemical constituents within complex, and even simple, mixtures would not be possible. To underscore the general importance of chromatography, two examples will suffice: our knowledge of mechanisms in organic chemistry (including that pertaining to lignin) would be very limited if products could not be isolated and quantified by chromatographic methods, and their structures subsequently determined. Similarly, without chromatography, our detailed understanding of biochemical pathways and metabolic products would not be as highly developed as it is today.

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Authors
  1. N. G. Lewis
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Editors and Affiliations

  1. LignoTech USA Inc., 100 Highway 51 South, 54474, Rothschild, WI, USA

    Stephen Y. Lin

  2. 600 Black Walnut Court, 28792, Hendersonville, NC, USA

    Carlton W. Dence

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© 1992 Springer-Verlag Berlin Heidelberg

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Lewis, N.G. (1992). High Performance Liquid Chromatography (HPLC). In: Lin, S.Y., Dence, C.W. (eds) Methods in Lignin Chemistry. Springer Series in Wood Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74065-7_39

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  • DOI: https://doi.org/10.1007/978-3-642-74065-7_39

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74067-1

  • Online ISBN: 978-3-642-74065-7

  • eBook Packages: Springer Book Archive

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