Abstract
The membrane topology of the β subunit of Na+/K+-ATPase suggests that the protein has one single membrane spanning segment about 35 amino-acids behind the N-terminus, followed by a huge extracellular domain. Therefore, a recombinant α1 subunit protein lacking the intracellular N-terminal amino-acids as well as the computer predicted transmembrane domain should be directed to the secretory pathway if provided with the appropriate signal peptide sequences at the new N-terminal end. We have chosen this approach for two reasons. First, secretion into the culture medium would clearly demonstrate that the β subunit of the Na+/K+- ATPase β subunit is anchored by one single transmembrane segment. Second, since structural analysis of membrane anchored proteins is still a difficult task to solve, the secreted extracellular domain could be a suitable target for such analysis as has been shown for example with the extracellular domain of CD4 (1).
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References
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© 1994 Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt
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Gloor, S., Nasse, K. (1994). Synthesis and secretion of the extracellular domain of the β1 subunit isoform of NA+/K+-ATPase into the culture medium of CHO cells. In: Bamberg, E., Schoner, W. (eds) The Sodium Pump. Steinkopff. https://doi.org/10.1007/978-3-642-72511-1_33
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DOI: https://doi.org/10.1007/978-3-642-72511-1_33
Publisher Name: Steinkopff
Print ISBN: 978-3-642-72513-5
Online ISBN: 978-3-642-72511-1
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