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Analysis of Platelet, Tumor Cell and Endothelial Cell Interactions in Vivo and in Vitro

  • Conference paper
Prostaglandins in Cancer Research

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Perhaps the most important obstacle to the improved survival of cancer patients is disseminated spread of the disease (metastasis), which is the culmination of a complex series of events referred to collectively as the metastatic cascade. During the growth of the initial tumor mass, many cells become detached and migrate into the surrounding normal tissue (invasion). Some tumor cells migrate into the circulatory system (intravasation) and are carried throughout the patient. Once in the circulation, tumor cells can pass freely between the circulatory and lymphatic systems (Hilgard et al. 1972). However, patients having a large population of circulating tumor cells do not necessarily have a poor prognosis (Salsbury 1975), since fewer than 0.001% of the circulating tumor cells are able to attach to the endothelium or subcellular matrix of the vessel wall (arrest and adhesion), migrate into the surrounding tissue (extravasation), and form secondary tumors (Weiss 1980, 1982, 1983). It would therefore appear that the arrest and adhesion steps of the metastatic cascade are critical to the formation of metastases and could be a favorable target for antimetastatic therapy regimens.

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Abbreviations

ADP:

adenosine 5′-diphosphate

MEC:

microcarrier-grown endothelial cells

PDGF:

platelet-derived growth factor

PGI2 :

prostacyclin

TCIPA:

tumor cell-induced platelet aggregation

TXA2 :

thromboxane A2

TXSI:

thromboxane A2 synthase inhibitor

W256:

Walker 256 carcinosarcoma

125I-Udr:

5-[125I]-iododeoxyuridine.

References

  • Bastida E, Ordinas A, Giardina SL, Jamieson GA (1982) Differentiation of platelet aggregating effects of human tumor cell lines based on inhibition studies with apyrase, hirudin, and phospholipase. Cancer Res 42: 4348–4352

    PubMed  CAS  Google Scholar 

  • Brain MC, Azzopardi JG, Baker LRI, Pinco GF, Roberts PD, Dacic JV (1970) Microangiopathic haemolytic anemia and mucin-forming adenocarcinoma. Br J Haematol 18: 183–190

    Article  PubMed  CAS  Google Scholar 

  • Cavanaugh PG, Sloane BF, Bajkowski AS, Gasic GJ, Gasic TB, Honn KV (1983) Involvement of a cathepsin B-like cysteine proteinase in platelet aggregation induced by tumor cells and their shed membrane vesicles. Clin Exp Metastasis 1: 297–307

    Article  PubMed  CAS  Google Scholar 

  • Dexter DL, Kowalski HM, Blazar HA, Fligiel A, Heppner GH (1978) Heterogeneity of tumor cells from a single mouse mammary tumor. Cancer Res 38: 3174–3179

    PubMed  CAS  Google Scholar 

  • Edwards RL, Rickles FR (1984) Hemostatic alterations in cancer patients. In: Honn KV, Sloane BF (eds) Hemostatic Mechanisms of Metastasis. Martinus Nijhoff, The Hague, pp 342–354

    Chapter  Google Scholar 

  • Gasic GJ (1984) Role of plasma, platelets and endothelial cells in tumor metastasis. Cancer Metastasis Rev 3: 99–116

    Article  PubMed  CAS  Google Scholar 

  • Gasic GJ, Gasic TB, Stewart CC (1968) Antimetastatic effects associated with platelet reduction. Proc Natl Acad Sci USA 61: 46–52

    Article  PubMed  CAS  Google Scholar 

  • Grignani G, Pacehiarini L, Almasio P, Pagliasino M, Gamba G (1984) Activation of platelet prostaglandin biosynthesis pathway during neoplastic cell induced platelet aggregation. Thromb Res 34: 147–157

    Article  PubMed  CAS  Google Scholar 

  • Hilgard P, Beyerle L, Hohage R, Hiemeyer V, Kubler M (1972) The effect of heparin on the initial phase of metastasis formation. Eur J Cancer 8: 347–352

    PubMed  CAS  Google Scholar 

  • Honn KV (1983) Inhibition of tumor cell metastasis by modulation of the vascular prostacyclin/ thromboxane A2 system. Clin Exp Metastasis 1: 103–114

    Article  PubMed  CAS  Google Scholar 

  • Honn KV, Sloane BF (1983) Hemostatic Mechanisms and Metastasis. Martinus Nijhoff, The Hague

    Google Scholar 

  • Honn KV, Cicone B, Skoff A (1981) Prostacyclin: a potent antimetastatic agent. Science 212: 1270–1272

    Article  PubMed  CAS  Google Scholar 

  • Honn KV, Menter D, Cavanaugh PG, Neagos G, Moilanen D, Taylor JD, Sloane BF (1983a) A review of prostaglandins and the treatment of tumor metastasis. Acta Clin Belg 38: 53–67

    PubMed  CAS  Google Scholar 

  • Honn KV, Menter D, Moilanen D, Cavanaugh PG, Taylor JD, Sloane BF (1983b) Role of prostacyclin and thromboxanes in tumor cell-platelet-vessel wall interactions. In: McBrein DCH, Slater TF (eds) Protective Agents in Cancer. Academic Press, London, pp 57–59

    Google Scholar 

  • Honn KV, Menter DG, Onoda JM, Taylor JD, Sloane BF (1984) Role of prostacyclin as a natural deterrent to hematogenous tumor metastasis. In: Nicolson GL, Milas L (eds) Cancer Invasion and Metastasis: Biologic and Therapeutic Aspects. Raven, New York, pp 361–368

    Google Scholar 

  • Honn KV, Onoda JM, Menter DG, Cavanaugh PG, Taylor JD, Crissman JD, Ryan RE, Sloane BF (1986) Possible strategies for antimetastatic therapy. In: Welch DR, Bhuyan BK, Liotta LA (eds) Cancer Metastasis. Experimental and Clinical Strategies. Alan R Liss, New York, pp 217–246

    Google Scholar 

  • Honn KV, Menter DG, Onoda JM, Steinert BW, Diglio CA, Taylor JD, Sloane BF (1987) Tumor cell-platelet-endothelial cell interactions and prostaglandin metabolism. In: Samuelsson B, Paoletti R, Ramwell PW (eds) Advances in Prostaglandin, Thromboxane, and Leukotriene Research. Raven, New York, pp 977–981

    Google Scholar 

  • Jamieson GA (1982) Interaction of Platelets and Tumor Cells. Alan R Liss, New York

    Google Scholar 

  • Karniguian A, Simmons P, Legrand YJ, Moncada S, Caen JP (1982) A comparison of the inhibitory effect of prostacyclin and carbacyclin on platelet adhesion to collagen. Prostaglandins 24: 827–836

    Article  PubMed  CAS  Google Scholar 

  • Laghi F, DiRoberto PF, Panici PB, Margariti PI, Scribano D, Cudillo L, Villani L, Bizzi B (1983) Coagulation disorders in patients with tumors of the uterus. Tumori 69: 349–353

    PubMed  CAS  Google Scholar 

  • Lipton A, Cano C, Leitzel K (1984) Mitogenic stimulation of tumor cells by platelet derived growth factors. In: Honn KV, Sloane BF (eds) Hemostatic Mechanisms and Metastasis. Martinus Nijhoff, The Hague, pp 266–281

    Chapter  Google Scholar 

  • Menter D, Neagos G, Dunn J, Palazzo R, Tchen TT, Taylor JD, Honn KV (1982) Tumor cell induced platelet aggregation: inhibition by prostacyclin, thromboxane A2 and phosphodiesterase inhibitors. In: Powles TJ, Bockman RS, Honn KV, Ramwell PW (eds) Prostaglandins and Cancer. Alan R Liss, New York, pp 809–814

    Google Scholar 

  • Menter DG, Onoda JM, Taylor JD, Honn KV (1984) Effects of prostacyclin on tumor cell induced platelet agpegation. Cancer Res 44: 450–456

    PubMed  CAS  Google Scholar 

  • Menter DG, Hatfield JS, Harkins C, Sloane BF, Taylor JD, Crissman JD, Honn KV (1987) Tumor cell-platelet interactions in vitro and their relationship to in vivo arrest of hematogenously circulating tumor cells. Clin Exp Metastasis (in press)

    Google Scholar 

  • Miner KM, Kawaguchi T, Uba GW, Nicolson GL (1982) Clonal drift of cell surface, melanogenic, and experimental metastatic properties of in vivo selected, brain meninges-colonizing murine B16 melanoma. Cancer Res 42: 4631–4638

    PubMed  CAS  Google Scholar 

  • Moncada S, Vane JR (1977) The role of prostacyclin in vascular tissue. Fed Proc 38: 66–71

    Google Scholar 

  • Pearlstein E, Ambrogio C, Gasic G, Karpatkin S (1981) Inhibition of the platelet-aggregating activity of two human adenocarcinomas of the colon and an anaplastic murine tumor with a specific thrombin inhibitor, dansylarginine N-(3-ethyl-l,5-pentanediyl)amide. Cancer Res 41: 4535–4539

    PubMed  CAS  Google Scholar 

  • Rickles FR, Edwards RL (1983) Activation of blood coagulation in cancer: Trousseau’s syndrome revisited. Blood 62: 14–31

    Google Scholar 

  • Rowland RN, Donovan MJ, Picciano PT, Kreutzer DL (1984a) Fibrin-mediated vascular injury: demonstration of vascular endothelial cell retraction in response to soluble fibrin-associated factors. J Exp Pathol 3: 217–240

    Google Scholar 

  • Rowland FN, Donovan MJ, Picciano PT, Wilner GD, Kreutzer DL (1984b) Fibrin-mediated vascular injury. Identification of fibrin peptides that mediate endothelial cell retraction. Am J Pathol 117: 418–428

    Google Scholar 

  • Sacchi A, Mauro F, Zupi G (1984) Changes of phenotypic characteristics of variants derived from Lewis lung carcinoma during long-term in vitro growth. Clin Exp Metastasis 2: 171–178

    Article  PubMed  CAS  Google Scholar 

  • Salsbury J A (1975) The significance of the circulating cancer cell. Cancer Treat Rev 2: 55–72

    Article  PubMed  CAS  Google Scholar 

  • Sindelar WR, Tralka TS, Ketcham AS (1975) Electron microaeopic observations on formation of pulmonary metastases. J Surg Res 18: 137–161

    Article  PubMed  CAS  Google Scholar 

  • Sloane BF, Honn KV, Sadler JG, Turner WA, Kimpson JJ, Taylor JD (1982) Cathepsin B activity in B16 melanoma cells: a possible marker for metastatic potential. Cancer Res 42: 980–986

    PubMed  CAS  Google Scholar 

  • Talmadge JE, Meyers KM, Priener DJ, Starkey JR (1980) Role of NK cells in tumor growth and metastasis in beige mice. Nature 284: 622–624

    Article  PubMed  CAS  Google Scholar 

  • Weiss L (1980) Cancer cell traffic from the lungs to the liver: an example of metastatic inefficiency. Int J Cancer 25: 385–392

    Article  PubMed  CAS  Google Scholar 

  • Weiss L (1982) Metastatic inefficiency. In: Liotta LA, Hart IR (eds) Tumor Invasion and Metastasis. Martinus Nijhoff, The Hague, pp 81–98

    Chapter  Google Scholar 

  • Weiss L (1983) Overview of the metastatic cascade. In: Honn KV, Sloane BF (eds) Hemostatic Mechanisms of Metastasis. Martinus Nijhoff, The Hague pp 15–38

    Google Scholar 

  • Weiss L, Dimitrov DS, Angelova M (1985) The hemodynamic destruction of intravascular cancer cells in relation to myocardial metastasis. Proc Natl Acad Sci USA 82: 5357–5341

    Article  Google Scholar 

  • Welch DR, Milas L, Tomasovic SP, Nicolson GL (1983) Heterogenous response and clonal drift of sensitivities of metastatic 13762F mammary adenocarcinoma clones to gamma radiation in vitro. Cancer Res 43: 6–10

    PubMed  CAS  Google Scholar 

  • Whittle BJR, Moncada S, Whiting F, Vane JR (1980) Carbacyclin-a potent stable prostacyclin analogue for the inhibition of platelet aggregation. Prostaglandins 19: 605–627

    Article  PubMed  CAS  Google Scholar 

  • Zacharski LR, Rickles FR, Henderson WG, Martin JF, Forman WB, van Eeckhoust JP, Cornell CJ, Forcier RJ (1982) Platelets and malignancy. Rationale and experimental design for the VA Cooperative Study of RA-233 in the treatment of cancer. Am J Clin Oncol 5: 593–609

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Radiation Oncology, Wayne State University, Detroit, MI, 48202, USA

    B. F. Sloane

Authors
  1. K. V. Honn
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  2. D. G. Menter
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  3. B. W. Steinert
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  4. J. D. Taylor
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  5. J. M. Onoda
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  6. B. F. Sloane
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Editor information

Editors and Affiliations

  1. Department of Experimental Medicine, II University of Rome, Via O. Raimondo, 00173, Rome, Italy

    Enrico Garaci

  2. Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133, Milan, Italy

    Rodolfo Paoletti

  3. Institute of Experimental Medicine, CNR, II University of Rome, Via O. Raimondo, 00173, Rome, Italy

    M. Gabriella Santoro

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© 1987 Springer-Verlag Berlin Heidelberg

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Honn, K.V., Menter, D.G., Steinert, B.W., Taylor, J.D., Onoda, J.M., Sloane, B.F. (1987). Analysis of Platelet, Tumor Cell and Endothelial Cell Interactions in Vivo and in Vitro. In: Garaci, E., Paoletti, R., Santoro, M.G. (eds) Prostaglandins in Cancer Research. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71904-2_15

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  • DOI: https://doi.org/10.1007/978-3-642-71904-2_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71906-6

  • Online ISBN: 978-3-642-71904-2

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