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On the Formation and Biological Role of Leukotrienes and Lipoxins

  • B. Samuelsson
  • C. Serhan
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

The oxygenation of arachidonic acid and other polyunsaturated fatty acids by a wide variety of cells results in the formation of several structurally distinct classes of biologically active compounds (Samuelsson 1983; Samuelsson et al. 1985). These compounds include the prostaglandins, thromboxanes, leukotrienes, and other oxygenated derivatives of polyunsaturated fatty acids. A most recent addition to this family of biologically active compounds is the lipoxins (Serhan et al. 1984a). Both leukotrienes and lipoxins are formed by transformations which involve lip oxygenation. In general, lipoxygenase products display a wide range of biological actions and appear to be involved in immunity, the regulation of inflammation, and other physiological and pathophysiological processes. This chapter describes results of recent studies on the 5-lipoxygenase (a key enzyme complex involved in the formation of these compounds) and the biosynthesis, stereochemistry and biological activities of the lipoxins.

Keywords

Arachidonic Acid Human Leukocyte Eicosatetraenoic Acid Silicic Acid Chromatography Lung Strip 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Borgeat P, Hamberg M, Samuelsson B (1976) Transformation of arachidonic acid and homo-γ-linolenic acid by rabbit polymorphonuclear leukocytes. J Biol Chem 251:7816–1729. Correction: JBC (1977) 252: 8772Google Scholar
  2. Dahlén SE, Raud J, Serhan CN, Björk J, Samuelsson B (1986) Biological activities of lipoxin A includes lung strip contraction and dilation of arterioles in vivo. Acta Physiol Scand (submitted for publication)Google Scholar
  3. Hansson A, Serhan CN, Ingelman-Sundberg M, Samuelsson B (1985) Activation of protein kinase C by lipoxin A and other eicosanoids: Intracellular action of oxygenation products of arachidonic acid. Biochem Biophys Res Commun 134: 1215–1222Google Scholar
  4. Hulting AL, Lindgren JA, Hökfelt T, Heidvall K, Eneroth P, Werner S, Patrono C, Samuelsson B (1985) Leukotriene C4 stimulates LH secretion from rat pituitary cells in vitro. Eur J Pharmacol 106: 459–460CrossRefGoogle Scholar
  5. Lindgren JÅ, Hökfelt T, Dahlen SE, Patrono C, Samuelsson B (1984) Leukotrienes in the rat central nervous system. Proc Natl Acad Sci USA 81: 6212–6216PubMedCrossRefGoogle Scholar
  6. Puustinen T, Serhan CN, Samuelsson B (1986) Evidence for a 5(6)-epoxy-tetraene intermediate in the biosynthesis of lipoxin B in human leukocytes. In: 6th Int Conf on Prostaglandins and Related Compounds, 3–6 June 1986, Florence, Italy (Abstract), p. 187Google Scholar
  7. Rådmark O, Shimizu T, Samuelsson B (1984) LTA4-hydrolase in human leukocytes. Purification and properties. J Biol Chem 259: 12339–12345Google Scholar
  8. Ramstedt U, Ng J, Wigzell H, Serhan CN, Samuelsson B (1985) Action of novel eicosanoids lipoxin A and B on human natural killer cell cytotoxicity: Effects on intracellular cAMP and target cell binding. J Immunol 135: 3434–3438Google Scholar
  9. Ramstedt U, Serhan CN, Nicolaou KC, Weber SE, Wigzell H, Samuelsson B (1986) Lipoxin A induced inhibition of natural killer cells: Studies on stereospecificity and mode of action. J Immunol (submitted for publication)Google Scholar
  10. Rouzer C, Samuelsson B (1986) 5-Lipoxygenase from human leukocytes associates with membrane in the presence of calcium. In: Samuelsson B, Ramwell P, Paoletti R (eds) Advances in Prostaglandin, Thromboxane and Leukotriene Research. Raven Press, New York, vol 17 (in press)Google Scholar
  11. Samuelsson B (1983) Leukotrienes: Mediators of immediate hypersensitivity reactions and inflammation. Science 220: 568–575PubMedCrossRefGoogle Scholar
  12. Samuelsson B, Hammarström S, Hamberg M, Serhan CN (1985) Structural determination of leukotrienes and lipoxins. In: Pike JE, Morton DR (eds) Advancesin Prostaglandin, Thromboxane and Leukotriene Research. Raven Press, New York, vol 14, pp 45–71Google Scholar
  13. Serhan CN, Hamberg M, Samuelsson B (1984a) Trihydroxytetraenes: A novel series of compounds formed from arachidonic acid in human leukocytes. Biochem Biophys Res Commun 118: 943–949Google Scholar
  14. Serhan CN, Hamberg M, Samuelsson B (1984b) Lipoxins: Novel series of biologically active compounds formed from arachidonic acid in human leukocytes. Proc Natl Acad Sci USA 81: 5335–5339Google Scholar
  15. Serhan CN, Hamberg M, Samuelsson B (1985) Novel mechanisms in the arachidonic acid cascade: Formation of lipoxins. In: Russo-Marie F (ed) Advances in Inflammation Research. Raven Press, New York, vol 10, pp 117–128Google Scholar
  16. Serhan CN, Hamberg M, Samuelsson B, Morris J, Wishka J (1986a) On the stereochemistry and biosynthesis of lipoxin B. Proc Natl Acad Sci USA 83: 1983–1987PubMedCrossRefGoogle Scholar
  17. Serhan CN, Nicolaou KC, Webber SE, Veale CA, Dahlen SE, Puustinen TJ, Samuelsson B (1986b) Lipoxin A: Stereochemistry and biosynthesis. J Biol Chem (submitted for publication )Google Scholar
  18. Serhan CN, Nicolaou KC, Webber SE, Veale CA, Haeggström J, Puustinen TJ, Samuelsson B (1986c) Stereochemistry and biosynthesis of lipoxins. In: Samuelsson B, Ramwell P, Paoletti R (eds) Advances in Prostaglandin, Thromboxane and Leukotriene Research. Raven Press, New York, vol 17 (in press)Google Scholar
  19. Shimizu T, Ridmark O, Samuelsson B (1984) Enzyme with dual lipoxygenase activities catalyzes leukotriene A4 synthesis from arachidonic acid. Proc Natl Acad Sci USA 81: 689–693PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • B. Samuelsson
    • 1
  • C. Serhan
    • 1
  1. 1.Department of Physiological ChemistryKarolinska InstituteStockholmSweden

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