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The Effects of Fiber Length on the In Vitro Cytotoxicity of Asbestos Samples in Three Different Assay Systems

  • I. P. Gormley
  • G. M. Brown
  • H. Cowie
  • A. Wright
  • J. M. G. Davis
Part of the NATO ASI Series book series (volume 3)

Abstract

The ability of mineral fibers to cause disease in man is well established and the work of Stanton et al. (1977), Stanton and Layard (1978) and Pott (1978) has indicated that the ability of a dust to induce intrapleural tumors depends on the number of fibers in the sample that are greater than 8 pm in length and less than 1.5 μm in diameter.

Keywords

A549 Cell Fiber Diameter Fiber Length Mineral Dust Fiber Aspect Ratio 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beck EG, Tilkes F (1980) In Vitro effects of defined mineral fibres. In: Brown RC, Gormley IP, Chamberlain M, Davies R (eds). The in vitro effects of mi-neral dusts. Academic Press, London, p339Google Scholar
  2. Beckett ST (1975) The generation and evaluation of UICC asbestos clouds in animal exposure chambers. Ann occup Hyg 18: 187–198PubMedCrossRefGoogle Scholar
  3. Brown RC, Chamberlain M, Griffiths DM, Timbrell V (1978) The effect of fibre size on the in vitro biological activity of three types of amphibole asbestos. Int J Cancer 22: 721–727PubMedCrossRefGoogle Scholar
  4. Brown RC, Chamberlain M, Davies R, Gaffen J, Skidmore JW (1979) In vitro biological effects of glass fibre. J Environ Pathol Toxicol 2: 1369–1383PubMedGoogle Scholar
  5. Chamberlain M, Brown RC (1978) The cytotoxic effects of asbestos and other mineral dust in tissue culture cell lines. Brit J exp Pathol 59: 183–189Google Scholar
  6. Chamberlain M, Brown RC, Davies R, Griffiths DM (1979) In vitro prediction of the pathogenicity of mineral dusts. Brit J exp Pathol 60: 320–327Google Scholar
  7. Davis JMG, Bolton RE, Cowie H, Donaldson K, Gormley IP, Jones AD, Wright A (1984) These proceedingsGoogle Scholar
  8. Gormley IP, Collings P, Davis JMG, Ottery J (1979) An investigation into the cytotoxicity of respirable dusts from British collieries. Brit J exp Pathol 60: 526–535Google Scholar
  9. Gormley IP, Bolton RE, Brown GM, Davis JMG, Wright A (1983) Some observations on the in vitro cytotoxicity of chrysotile prepared by the wet dispersion process. Environ Health Perspect 51: 35–39PubMedCrossRefGoogle Scholar
  10. Pott F (1978) Some aspects on the dosimetry of the carcinogenic potency of asbestos and other fibrous dusts. Staub-Reinhalt Luft 38: 468–490Google Scholar
  11. Robock K (1973) Standard quartz DQ12<5μm for experimental pneumoconiosis research projects in the Federal Republic of Germany. Ann occup Hyg 16:63–66Google Scholar
  12. Schneider T (1978) The influence of counting rules on the number and on the size distribution of fibres. Ann occup Hyg 21: 341–350PubMedCrossRefGoogle Scholar
  13. Siegel S (1956) Non-parametric statistics for the behavioural sciences, McGraw-Hill, New York, p202Google Scholar
  14. Stanton MF, Layard M, Tegeris A, Miller E, May M, Kent E (1977) Carcinogenicity of fibrous glass: pleural response in the rat in relation to fibre dimension. J Natl Cancer Inst 58: 587–603PubMedGoogle Scholar
  15. Stanton MF, Layard M (1978) The carcinogenicity of fibrous minerals. In: Gravatt CC, La Fleur PD, Heinreich KFJ (eds). Workshop on Asbestos: Definition and Measurement Methods. National Bureau of Standards Special Publication No. 506. National Measurements Laboratory Washington DC, p143Google Scholar
  16. Wade JM, Lipkin LE, Stanton MF, Frank AL (1980) P388D1 in vitro cytotoxicity assay as applied to asbestos and other minerals: its possible relevance to carcinogenicity. In: Brown RC, Gormley IP, Chamberlain M, Davies R (eds). The In Vitro Effects of Mineral Dusts. Academic Press, London, p351Google Scholar
  17. Wagner JC, Berry G, Timbrell V (1973) Mesothelioma in rats after inoculation with asbestos and other material. Brit J Cancer 28: 173–185PubMedCrossRefGoogle Scholar
  18. Wright A, Gormley IP, Collings PL, Davis JMG (1980) The cytotoxicity of asbestos and other fibrous dusts. In: Brown RC, Gormley IP, Chamberlain M, Davies R (eds). The In Vitro Effects of Mineral Dusts. Academic Press, London, p25Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • I. P. Gormley
    • 1
  • G. M. Brown
    • 1
  • H. Cowie
    • 1
  • A. Wright
    • 1
  • J. M. G. Davis
    • 1
  1. 1.Institute of Occupational MedicineEdinburghUK

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