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Immune (y) Interferon Produced by Murine T-Cell Lymphomas

  • Santo Landolfo
  • Bernd Arnold
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Various cloned murine T-cell hybridomas and T lymphomas were evaluated for their ability to produce immune (y) IFN and other lymphokines such as interleukin-2 (IL-2) and macrophage activiating factor (MAF). The 2 T-cell clones, L12-R1 and -R4, dereived from a spontaneously in vitro transformed cell line L12 which was originally established from fetal calf serum (FCS)-primed C57BL/6 spleen cells (Rubin et al., 1980, Scand. J. Immunol. 12:407) were found to produce IFN after mitogen stimulation (1.000 IU) in presence of 2% FCS. By contrast in complete absence of FCS IFN titres decreased to 500 IU. None of the cell lines tested produced IFN either constitutively or upon lipopolysaccharide (LPS) stimulation. The IFN was characterized as immune (y) IFN by being labile at pH 2 and neutralized by 2 rabbit anti-murine IFN-y antisera but not by antiserum to murine IFN-α and -β. L12-R4 IFN-y chromatography on Sephacryl S-200 and Phenyl Sepharose columns indicates a molecular weight of about 45.000 and a significant apparent hydrophobicity just as other murine IFN-y. In addition to IFN-y IL-2 and MAF were also found in the supernatants of the mitogen treated L12-R4 cells. Our data provide further evidence that IFN-y is a product of T cells of the Lyt-1+, 23- phenotype and establish a system which could be used for the IFN-y purification as well as for the characterization of the molecular mechanisms involved in T cell differentiation leading to IFN production.

Keywords

Fetal Calf Serum Migration Inhibition Factor Phorbol Myristate Acetate Phorbol Myristate Acetate Transform Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    De Weck, A., F. Kristeenen and M. Landy (eds.)(1980) Biomedical Characterization of Lymphokines. Academic Press, New York.Google Scholar
  2. 2.
    Cohen, S., E. Pick and J.J. Oppenheim (eds.) (1979) Biology of the Lymphokines. Academic Press, New York.Google Scholar
  3. 3.
    Rubin, B., M.A. Cooley, C. Le Bourgne deKaouel, R.B. Taylor and P. Golstein. (1980) Scan. J. Immunol. 12, 401.CrossRefGoogle Scholar
  4. 4.
    Baron, S. and F. Bianzani (1982) The Interferon System. Tex. Rep. Biomed., Galveston, USA.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Santo Landolfo
    • 1
  • Bernd Arnold
    • 2
  1. 1.Institute of MicrobiologyUniversity of TorinoTorinoItaly
  2. 2.Institut für Immunologie und GenetikDeutsches KrebsforschungszentrumHeidelbergGermany

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