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Hormonally Defined Media

A Tool in Cell Biology Lectures and Posters Presented at the First European Conference on Serum-Free Cell Culture Heidelberg, October 7–9, 1982

  • G. Fischer
  • R. J. Wieser
Conference proceedings

Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Table of contents

  1. Front Matter
    Pages I-XIV
  2. Opening Lecture

    1. Front Matter
      Pages 1-1
  3. Nutritional Requirements

  4. Cell Proliferation and Growth Factors

    1. Front Matter
      Pages 45-45
    2. Robert R. Bürk
      Pages 57-67
    3. T. Maciag, R. Weinstein
      Pages 68-78
    4. J. Pouysségur, J. C. Chambard, A. Franchi, G. L’Allemain, S. Paris, E. Van Obberghen-Schilling
      Pages 88-102
    5. C. Betsholtz, C.-H. Heldin, Å. Wasteson, B. Westermark
      Pages 103-106
    6. Angela M. Otto, Marit Nilsen-Hamilton, Barbara Boss, Luis Jimenez de Asua
      Pages 120-122
    7. J. Straczek, M. H. Heulin, F. Sarem, A. Lasbennes, M. Artur, C. Geschier et al.
      Pages 127-130
  5. Cell-Substratum Interactions

  6. Neural Cells

  7. Endocrine and Exocrine Cells

    1. Front Matter
      Pages 233-233
    2. Mihir R. Banerjee, Prabir K. Majumder, Michael Antoniou, Jay Joshi
      Pages 234-249
    3. Elke Lang, Klaus Lang, Ulrich Krause, Kurt Racké, Bernd Nitzgen, Gerd Brunner
      Pages 250-263
    4. Joseph Orly, Patricia Weinberger-Ohana, Yigal Farkash
      Pages 274-284
  8. Hemopoietic and Immune System

    1. Front Matter
      Pages 299-299
    2. John Farrant, Christine A. Newton, Christine Weyman, Margaret North, Malcolm K. Brenner
      Pages 300-303
    3. Inge Flesch, Uwe-Peter Ketelsen, Ernst Ferber
      Pages 304-306
    4. Jacques A. Hatzfeld, Antoinette Hatzfeld
      Pages 307-309
    5. Tomoyuki Kawamoto, J. Denry Sato, Anh Le, Don B. McClure, Gordon H. Sato
      Pages 310-313
    6. Santo Landolfo, Bernd Arnold
      Pages 314-316
    7. Carlo Riccardi, Graziella Migliorati, Luigi Frati
      Pages 317-319
    8. G. Vinci, N. Casadevall, C. Lacombe, J. Chapman, W. Vainchenker, B. Varet et al.
      Pages 320-323

About these proceedings

Introduction

Until some years ago serum or crude tissue extracts were used pre­ dominantly or exclusively as media supplements for the cultivation of cells. However, during this time evidence accumulated that these sup­ plements could not provide in an optimal way most of the cultivated cells with all factors necessary for their survival, their prolifer­ ation and/or differentiation. Moreover, a variety of cells could not be cultivated at all under these conditions and often the composition of the cultures changed within rather short periods of time by overgrowth of initially present subpopulations of those cells which grow well in these supplements, as for example fibroblasts. Nevertheless, using these supplements (or fractions thereof), insight could be gained into some of the influences of serum or tissue extract constituents with re­ gard to survival, proliferation and differentiation of cells in cul­ ture. It became obvious from these experiments that serum or tissue extracts did not only supply cells with nutrients or vitamins (which are now constituents of all basic media), but also with hormones as well as growth-, differentiation-, and attachment-factors. In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti­ vate those cells which could not survive in serum-supplemented media alone. Under normal conditions in an organism, however, only a small population of cells has direct contact with serum.

Keywords

Fischer Hormonhaltiger serumfreier Nährboden Zellkultur biology cell cell biology cell culture influence tissue

Editors and affiliations

  • G. Fischer
    • 1
  • R. J. Wieser
    • 2
  1. 1.Institut für NeurobiologieUniversität HeidelbergHeidelberg 1Germany
  2. 2.Institut für PharmakologieUniversität MainzMainzGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-69290-1
  • Copyright Information Springer-Verlag Berlin Heidelberg 1983
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-69292-5
  • Online ISBN 978-3-642-69290-1
  • Series Print ISSN 0172-6625
  • Buy this book on publisher's site
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