Control by the Extracellular Environment of Differentiation Pathways in an Embryonal Carcinoma Cell Line

  • Michel Darmon
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


The embryonal carcinoma (EC) cell line 1003 forms multidifferentiated tumors when injected into syngeneic mice but is apparently nullipotential when grown in vitro in conventional media. Hormone-supplemented defined media were designed as an attempt to mimic in vivo conditions. Such media actually allowed 1003 cells to differentiate in vitro. The phenotypes eventually obtained depended upon the composition of the medium. Both diffusible factors and attachment factors were shown to control the differentiation of 1003 cells. The appearance of differentiated phenotypes was found to be due to induction - and not selection - phenomena. However, because of the impossibility to obtain clonal cultures of 1003 cells in serum-free media, experiments allowing to find out whether the extracellular environment exerts its effects on these cells at the level of determination or at the level of terminal differentiation could not be designed.


Tyrosine Hydroxylase Extracellular Environment Embryonal Carcinoma Cell Clonal Culture Attachment Factor 
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  1. Brinster, R.: The effect of cells transferred into the mouse blastocyst on subsequent development. J.Exp.Med. 140: 1019–1056, 1974.CrossRefGoogle Scholar
  2. Darmon, M.: Laminin provides a better substrate than fibronectin for attachment, growth and differentiation of 1003 embryonal carcinoma cells. In vitro, in press.Google Scholar
  3. Darmon, M., and Serrero, G.: Isolation of two different fibroblastic cell types from the embryonal carcinoma cell line 1003. Study of tumorigenic properties, surface antigens and differentiation res-ponses to 5-Azacytidine and dexamethasone. Cold Spring Harbor Conferences on Cell Proliferation 10, in press.Google Scholar
  4. Darmon, M., Bottenstein, J., and Sato, G.: Neural differentiation following culture of embryonal carcinoma cells in a serum-free defined medium. Dev.Biol. 85: 463–473, 1981.PubMedCrossRefGoogle Scholar
  5. Darmon, M., Stallcup, W., and Pittman, Q.: Induction of neural differentiation by serum-deprivation in cultures of the embryonal carcinoma cell line 1003. Exp.Cell Res. 138: 73–78, 1982a.PubMedCrossRefGoogle Scholar
  6. Darmon, M., Satllcup, W., Pittman, Q., and Sato, G.: Control of diffe-rentiation pathways by the extracellular environment in an embryonal carcinoma cell line. Cold Spring Harbor Conferences on Cell Proliferation 9: in press.Google Scholar
  7. Darmon, M., Barret, A., Puymirat, J., and Faivre, A.: Cholinergic neurons and embryonic mesenchymal cells arise from the same population of precursor cells in cultures of embryonal carcinoma cells triggered to differentiate by serum-deprivation. Biology of the Cell 45, (1): 10, 1982c.Google Scholar
  8. Darmon, M., Buc-Caron, M.H., Paulin, D., and Jacob, F.: Control by the extracellular environment of differentiation pathways in 1003 embryonal carcinoma cells: study at the level of specific intermediate filaments. EMBO Journal, in press.Google Scholar
  9. Jacob, F.: Mouse teratocarcinoma and mouse embryo. Proc.R.Soc.Lond. B 201: 249–270, 1978.PubMedCrossRefGoogle Scholar
  10. Mintz, B., and Illmensee, K.: Normal genetically mosaic mice produced from malignant terarocarcinoma cells. Proc.Natl.Acad.Sci.U.S.A. 72: 3585–3589, 1975.PubMedCrossRefGoogle Scholar
  11. Papaioannou, V., McBurney, M., Gardner, R., and Evans, M.: Fate of teratocarcinoma cells injected into early mouse embryos. Nature 258: 70–73, 1975.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Michel Darmon
    • 1
  1. 1.Département de Biologie MoléculaireInstitut PasteurParis Cedex 15France

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