Abstract
Early studies with inhibitors of nucleic acid synthesis resulted in the proposal of a DNA intermediate called a “provirus” in the life cycle of RNA tumor viruses (14). Further studies have provided substantial evidence for the synthesis of proviral DNA (pDNA) upon infection of cells with RNA tumor viruses and a role for the provirus in virus replication and cellular transformation (3,4). The mechanics by which pDNA is synthesized and integrated into the host cell genome, however, remain to be elucidated. There are conflicting reports on the site of pDNA synthesis in an infected cell (5,9), and size and other characteristics of pDNA have not been established. In this report we summarize our results on the synthesis and characteristics of pDNA and the effect of ethidium bromide on integration of pDNA into host cell DNA.
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References
Ali, M. and Baluda, M. A. Synthesis of avian oncornavirus DNA in infected chicken cells. J. Virol., 13:1005, 1974.
Bader, A. V. Role of mitochondria in the production of RNA-containing tumor viruses. J.Virol., 11:314, 1973.
Baluda, M. and Nayak, D. P. DNA complementary to viral RNA in leukemic cells induced by avian myeloblastosis virus. Proc. Natl. Acad. Sci. USA, 66:329, 1970.
Bishop, J. M. and Varmus, H. E. The molecular biology of RNA tumor viruses. In Becker, F., ed. Cancer: A Comprehensive Treatise. New York, Plenum Press (in press), 1974.
Dales, S. and Hanafusa, H. Penetration and intracellular release of the genomes of avian RNA tumor viruses. Virology, 50:323, 1972.
Duesberg, P. On the structure of RNA tumor viruses. Curr. Top. Microbiol. Immunol., 51:79, 1970.
Garapin, A. C., Varmus, H. E., Faras, A. J., Levinson, W. E., and Bishop, J. M. RNA-Directed DNA synthesis by virions of RSV: Further characterization of the templates and the extent of their transcription. Virology, 52:264, 1973.
Varmus, H. E., Guntaka, R. V., Fan, W. J. W., Heasley, S., and Bishop, J. M. Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus. Proc. Natl. Acad. Sci. USA, 71:3874, 1974.
Hatanaka, M., Kakefuda, T., Gilden, R. V., and Callan, E. A. O. Cytoplasmic DNA synthesis induced by RNA tumor viruses. Proc. Natl. Acad. Sci. USA, 68:1844, 1971.
Hirt, B. Selective extraction of polyoma DNA from infected mouse cell culture. J. Mol. Biol., 26:365, 1967.
Linial, M. and Mason, W. S. Characterization of two conditional early mutants of Rous sarcoma virus. Virology, 52:258, 1973.
Radloff, R., Bauer, W., and Vinograd, J. A dye-buoyant density method for the detection and isolation of closed circular duplex DNA: The closed circular DNA in HeLa cells. Proc. Natl. Acad. Sci. USA, 57:1514, 1967.
Richert, N. J. and Hare, J. D. Distinctive effects of inhibitors of mitochondrial function on Rous sarcoma virus replication and malignant transformation. Biochem. Biophys. Res. Commun., 46:5, 1972.
Temin, H. M. Mechanism of cell transformation by RNA tumor viruses. Ann. Rev. Microbiol., 25:609, 1971.
Temin, H. M. and Baltimore, D. DNA-Directed DNA synthesis and RNA tumor viruses. Adv. Virus Res., 17:219, 1972.
Temin, H. M. and Rubin, H. Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture. Virology, 6:669, 1958.
Varmus, H. E., Levinson, W. E., and Bishop, J. M. Extent of transcription by the RNA-dependent DNA polymerase of Rous sarcoma virus. Nature [New Biol.], 233:19, 1971.
Varmus, H. E., Vogt, P. K., and Bishop, J. M. Integration of DNA specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proc. Natl. Sci. USA, 70:3067, 1973.
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Guntaka, R.V., Bishop, J.M., Varmus, H.E. (1975). Synthesis and Integration of Rous Sarcoma Virus Proviral DNA in Duck Embryo Fibroblast Cells. In: Gottlieb, A.A., Plescia, O.J., Bishop, D.H.L. (eds) Fundamental Aspects of Neoplasia. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66112-9_24
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DOI: https://doi.org/10.1007/978-3-642-66112-9_24
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