Summary
Two procedures for the concentration of LCM virus without loss of infectivity are described: precipitation by zinc acetate and concentration by polyethylene glycol. Further purification is achieved by steric chromatography on controlled pore glass. At a pore size of 43.7 nm all the infectivity appears in the exclusion volume. This material is free of complement-fixing activity.
Non-infectious complement-fixing antigen is extracted with butanol from infected guinea-pig organs or L cells and concentrated by evaporation. Preparations with titers of 40,000 or more can thus be obtained from either source. The extracted material (ECFA) by itself does not induce antibodies or specific protection in animals. When emulsified with Freund’s complete adjuvant, complement-fixing antibody is induced in guinea-pigs or rabbits. This antibody can be boosted to high titers with soluble ECFA. Not a trace of neutralizing activity is demonstrable in the same sera. Guinea-pigs repeatedly inoculated with large quantities of high-titered ECFA with or without adjuvant are not protected against subsequent challenge with infectious virus. Sera specifically reacting with ECFA do not react with purified virus. The neutralizing activity of anti-LCM virus hyperimmune serum is not blocked by ECFA. Upon disintegration of the purified virus with urea and sodium dodecyl sulfate, complement-fixing activity is released, provided the starting material contained 109.5 1050 (L) / ml or more.
It is concluded that complement-fixing antigen as extracted with butanol from infected tissues or cells is not represented on the surface of the virus. Whether it is a structural component of the virion remains to be determined.
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Gschwender, H.H., Lehmann-Grube, F. (1973). Antigenic Properties of the LCM Virus: Virion and Complement-Fixing Antigen. In: Lehmann-Grube, F. (eds) Lymphocytic Choriomeningitis Virus and Other Arenaviruses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65681-1_3
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DOI: https://doi.org/10.1007/978-3-642-65681-1_3
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