Skip to main content
SpringerLink
Log in

Measurement of Renin and of Angiotensin (Extraction and Bioassay)

  • Chapter
Angiotensin

  • 116 Accesses

Abstract

Anyone entering the field of the renin-angiotensin system will be faced with a choice of many techniques for the determination of the different components of the system. These are so numerous that it is impossible for one group of investigators to have had direct experience of all the methods. To sort the useful from the less useful or poor ones, could be difficult indeed. However, it is the intention of the authors first to discuss the procedures for measurement of angiotensin and to describe here in some detail some useful techniques for measurement of renin activity or renin concentration. Angiotensin I-converting enzyme, angiotensinases radioimmunoassays are discussed in another chapter.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Arakawa, K., Minohara, A., Yamada, J., Uemura, N., Nakamura, M.: Micro-determination of human plasma renin activity with the addition of homologous substrate. Clin. chim. Acta 22, 309 (1968).

    Article  Google Scholar 

  • Bing, J.: Mechanisms of the increased sensitivity to renin in nephrectomized animals. Acta path, microbiol. scand. 60, 311 (1964).

    CAS  Google Scholar 

  • Blaquier, P., Hoobler, S. W., Schroeder, J., Gomez, A., Rreulen, T.: Effect of bilateral nephrectomy on pressor response to renin. Amer. J. Physiol. 203, 339 (1962).

    PubMed  CAS  Google Scholar 

  • Blaufox, M., Birbari, A., Hickler, R. B., Merrill, J. P.: Peripheral plasma renin activity in renal homotransplant recipients. New Engl. J. Med. 275, 1165 (1965).

    Article  Google Scholar 

  • Boucher, R., Biron, P., Genest, J.: Procedure for isolation and determination of human blood angiotensin. Canad. J. Biochem. 39, 581 (1961).

    Article  CAS  Google Scholar 

  • Boucher, R., Kurihara, H., Grise, C., Genest, J.: Measurement of plasma angiotensin I converting enzyme activity. Circulât. Res. XXVI and XXVII, I–83 (1970).

    Google Scholar 

  • Boucher, R., Menard, J., Genest, J.: A micromethod for measurement of renin in the plasma and kidney of rats. Can. J. Physiol. Pharmacol. 45, 881 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Boucher, R., Saidi, M., Genest, J.: A new “angiotensin I converting enzyme” system. In: Hypertension 1972 (GENEST, J., Koiw, E., Eds.). Berlin-Heidelberg-New York: Springer 1972.

    Google Scholar 

  • Boucher, R., Veyrat, R., de Champlain, J., Genest, J.: New procedures for measurement of human plasma angiotensin and renin activity levels. Canad. med. Ass. J. 90, 194 (1964).

    PubMed  CAS  Google Scholar 

  • Brown, J. J., Davies, D. L., Lever, A. F., Robertson, J. I. S.: (1) Variations in plasma renin concentration in several physiological and pathological states. Canad. med. Ass. J. 90, 201 (1964).

    PubMed  CAS  Google Scholar 

  • Brown, J. J., Davies, D. L., Lever, A. F., Robertson, J. I. S., Tree, M.: (2) The estimation of renin in human plasma. Biochem. J. 93, 594 (1964).

    PubMed  CAS  Google Scholar 

  • Bumpus, F. M., Schwarz, H., Page, I. H.: Partial separation of an oxytocic principle from preparations of angiotonin. Circulât. Res. 4, 488 (1956).

    PubMed  CAS  Google Scholar 

  • Cohn, J. N., Notargiacomo, A. V.: Clinical application of a simple specific bioassay technique for measuring renin activity. Amer. J. med. Sci. 257, 344 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Conn, J. W., Cohen, E. L., Rovner, D. R.: Suppression of plasma renin activity in primary aldosteronism. J. Amer. med. Ass. 190, 213 (1964).

    CAS  Google Scholar 

  • Cook, W. F., Lee, M. R.: The preparation of rabbit renin-substrate for the assay of minute amounts of renin. Biochem. J. 96, 413 (1965).

    PubMed  CAS  Google Scholar 

  • Ganten, D., Marquez-julio, A., Granger, P., Hayduk, K., Karsunky, K. P., Boucher, R., Genest, J.: Renin in Dog Brain. Amer. J. Physiol. 221 (6), 1733 (1971).

    Google Scholar 

  • Gocke, D. J., Sherwood, L. M., Openhoff, I., Gerten, J., Laragh, J. H.: Measurement of plasma angiotensin II and correlation with renin activity. J. Clin. Endocr. 28, 1675 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Gould, A. B., Skeggs, L. T., Kahn, J. R.: Measurement of renin and substrate concentrations in human serum. Lab. Invest. 15, 1802 (1966).

    PubMed  CAS  Google Scholar 

  • Granger, P., Boucher, R., Genest, J.: Method for determination of renin activity in dog’s plasma and renal tissue. Can. J. Physiol. Pharmacol. 48 (7), 457 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Gregerman, R. I., Weaver, T., Kowatch, M. A.: Behavior of angiotensin octapeptides and dinitrophenyl-angiotensin during chromatography on polyacrylamide and dextran gels. J. Chromatog. 47, 369 (1970).

    Article  CAS  Google Scholar 

  • Gunnels, J. C., Grim, C. E., Robinson, R. R., Wildermann, N. M.: Plasma renin activity in healthy subjects and patients with hypertension. Arch, intern. Med. 119, 232 (1967).

    Article  Google Scholar 

  • Haas, E., Gould, A. B., Goldblatt, H.: Estimation of endogenous renin in human blood. Lancet 1968 1, 657.

    Article  PubMed  CAS  Google Scholar 

  • Hayduk, K., Boucher, R., Genest, J.: Renin activity content in various tissues of dogs under different physiopathological states. Proc. Soc. exp. Biol. (N. Y.) 134, 252 (1970).

    CAS  Google Scholar 

  • Helmer, O. M., Judson, W. E.: The quantitative determination of renin in the plasma of patients with arterial hypertension. Circulation 27, 1050 (1963).

    PubMed  CAS  Google Scholar 

  • Imai, M., Sokabe, H.: A method for the determination of plasma renin activity in small samples. Jap. Heart J. 8, 607 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Iwai, J., Knudsen, K. D., Dahl, L. K.: Genetic influence on the renin-angiotensin system. J. exp. Med. 131, 543 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Kaulhausen, H., Fillmann, B., Brever, H.: Determination of renin activity in human plasma. Biochem. J. 8 (3), 254 (1970).

    CAS  Google Scholar 

  • Keutel, H. J., Outzen, H. C., Weaver, R. G.: Application of the renin-antirenin assay in experimental hypertension: A preliminary report. Invest. Urol. 5, 107 (1967).

    PubMed  CAS  Google Scholar 

  • Klaus, D., Heizmann, A.: Methoden fiir die Bestimmung der Reninaktivitàt im Plasma. Z. klin. Chem. 6, 1 (1968).

    CAS  Google Scholar 

  • Kohlstaedt, K. G., Page, I. H.:The liberation of renin by perfusion of kidneys following reduction of pulse pressure. J. exp. Med. 72, 201 (1940).

    Article  PubMed  CAS  Google Scholar 

  • Lee, M. R., Cook, W. F., Menzie, J. K.: A sensitive method for assay of plasma renin activity. Circulât. Res. XIX, 260 (1966).

    Google Scholar 

  • Leloir, L. F., Muñoz, J. M., Braun-Menendez, E., Fasciolo, J. C.: (1) La secreción de renina y la formación de hypertensina. Rev. Soc. argent. Biol. 16, 75 (1940).

    Google Scholar 

  • Leloir, L. F., Muñoz, J. M., Braun-Menendez, E., Fasciolo, J. C.: (2) Dosaje de la renina. Rev. Soc. argent. Biol. 16, 635 (1940).

    CAS  Google Scholar 

  • Lemieux, R., Regoli, D.: Une méthode simple et spécifique pour la mesure de l’activité réninique du plasma chez l’homme. Un. méd. Can. 100, 727 (1971).

    CAS  Google Scholar 

  • Lever, A. F., Robertson, J. I. S., Tree, M.: The estimation of renin in plasma by an enzyme kinetic technique. Biochem. J. 91, 346 (1964).

    PubMed  CAS  Google Scholar 

  • Levine, M., Dorer, F. E., Kattn, J. R., Lentz, K. E., Skeggs, L. T.: Chemical determination of hog kidney renin by an automated method. Ann. Biochem. 34 (2), 366 (1970).

    Article  CAS  Google Scholar 

  • Mendelsohn, F. A., Johnston, C. I.: A radiochemical renin assay. Biochem. J. 121, 241 (1971).

    PubMed  CAS  Google Scholar 

  • Meurer, K. A., Ganten, D., Kauffmann, W.: A test for assessing the responsiveness of plasma renin activity. Germ. Med. Mth. V, 606 (1970).

    Google Scholar 

  • Miura, Y.: Measurement of renin activity in human plasma. Tohoku J. exp. Med. 98 (2), 125.

    Google Scholar 

  • Montague, D., Riniker, B., Brunner, H., Gross, F.: Synthesis and biological activities of a tetradecapeptide renin substrate. Amer. J. Physiol. 210 (3), 591 (1966).

    PubMed  CAS  Google Scholar 

  • Muñoz, J. M., Taquini, A. C., Braun-Menendez, E., Fasciolo, J. C., Leloir, L. F.: Método para la medicione de la renina humana. Rev. Soc. argent. Biol. 19, 321 (1943).

    Google Scholar 

  • Nash, F. D., Bailie, M. D., Wathen, R. L., Rostorfer, H. H.: An approach automated determination of renin activity in plasma. Automat. Anal. Chem. Technicon Symp. 1, 599 (1968).

    CAS  Google Scholar 

  • Page, I. H., Bumpus, F. M.: Angiotensin. Physiol. Rev. 41, 331 (1961).

    PubMed  CAS  Google Scholar 

  • Page, I. H., Mubbin, J. W.: Angiotensin. In: Renal Hypertension, pp. 64–98. Chicago: Year Book Medical Publishers, 1968.

    Google Scholar 

  • Paladini, A. C., Braun-Menendez, E., Del Frade, I. S., Massani, Z. M.: The estimation of angiotensin in blood. J. Lab. clin. Med. 53, 264 (1959).

    PubMed  CAS  Google Scholar 

  • Peart, W. S.: A new method of large scale preparation of hypertensin, with a note on its assay. Biochem. J. 59, 300 (1955).

    PubMed  CAS  Google Scholar 

  • Pickens, P. T., Bumpus, F. M., Lloyd, A. M., Smeby, R. R., Page, I. H.: Measurement of renin activity in human plasma. Circulât. Res. 17, 438 (1965).

    PubMed  CAS  Google Scholar 

  • Pickering, G. W., Prinzmetal, M.: Some observations on renin, a pressor substance contained in normal kidney, together with a method for its biological assay. Clin. Sci. 3, 211 (1938).

    CAS  Google Scholar 

  • Poulsen, K.: Kinetic and measurement of the renin-antirenin system. Acta path, microbiol. scand. 68, 197 (1966).

    CAS  Google Scholar 

  • Poulsen, K.: Microtechnique for quantitative measurement of renin in plasma. Acta path, microbiol. scand. 69, 19 (1967).

    Article  CAS  Google Scholar 

  • Poulsen, K.: Simplified method for radioimmunoassay of enzyme systems. Application on the human renin-angiotensin system. J. Lab. clin. Med. 78, 309 (1971).

    PubMed  CAS  Google Scholar 

  • Regoli, D.: Renin inactivation by human plasma. Can. J. Physiol. Pharmacol. 48 (6), 350.

    Google Scholar 

  • Regoli, D., Vane, J. R.: A sensitive method for the assay of angiotensin. Brit. J. Pharmacol. 23, 351 (1964).

    PubMed  CAS  Google Scholar 

  • Begoli, D., Vane, J. R.: The continuous estimation of angiotensin formed in the circulation of the dog. J. Physiol. 183, 513 (1966).

    Google Scholar 

  • Roth, M., Reinharz, A.: (1) Un dosage chimique de la renine. Helv. phvsiol. pharmacol. Acta 24, 115 (1966).

    Google Scholar 

  • Roth, M., Reinharz, A.: (2) Un substrat pour le dosage chimique de la renine. Helv. chim Acta 49, 1903 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Ryan, J. W., Menzie, J. K., Lee, M. R.: A rapid simple method for the assay of renin in rabbit plasma. Biochem. J. 108, 679 (1968).

    PubMed  CAS  Google Scholar 

  • Schaechtelin, G., Baechtold, N., Haefeli, L., Regoli, D., Gaudry-Paredes, A., Peters, G.: A renin-inactivating system in rat plasma. Amer. J. Physiol. 215, 632 (1968).

    PubMed  CAS  Google Scholar 

  • Schwyzer, R., Turrian, H.: The chemistry and pharmacology of angiotensin. Vitam. and Horm. 18, 237 (1960).

    Article  CAS  Google Scholar 

  • Scornik, O. A., Paladini, A. C., Braun-Menendez, E.: Further improvements on a method for the estimation of angiotensin in blood. Acta physiol. lat-amer. 10, 64 (1960).

    PubMed  CAS  Google Scholar 

  • Skeggs, L. T., Kahn, J. R., Shumway, N. P.: The preparation and function of the hyper-tensin converting enzyme. J. exp. Med. 103, 295 (1956).

    Article  PubMed  CAS  Google Scholar 

  • Skeggs, L. T., Lentz, K. E., Kahn, J. R., Hochstrasser, H.: Studies on the preparation and properties of renin. Circulat. Res. Supp. 21, II–91 (1967).

    Google Scholar 

  • Skeggs, L. T., Lentz, K. E., Kahn, J. R., Dorer, F. E., Levine, M.: Pseudorenin. A new angiotensin-forming enzyme. Circulat. Res. 25, 451 (l969).

    PubMed  CAS  Google Scholar 

  • Skeggs, L. T., Marsch, W. H., Kahn, J. R., Shumway, N. P.: The existence of two forms of hypertension. J. exp. Med. 99, 275 (1954).

    Article  PubMed  CAS  Google Scholar 

  • Skinner, S. L.: Improved assay methods for renin “concentration” and “activity” in human plasma. Methods using selective denaturation of renin substrate. Circulat. 1967, Res, 391.

    Google Scholar 

  • Smeby, R. R., Bumpus, F. M.: Renin. In: Proteolytic Enzymes, Vol. XIX, p. 699 (Perlmann, G. E., Lorand, L., Eds.). New York 1970.

    Google Scholar 

  • Sokabe, H.: Renin and euryhalinity in the Japanese eel. Internat. Cong. Physiol. Sc. 23, 392 (1965).

    Google Scholar 

  • Vallotton, M. B., Page, L. B., Haber, E.: Radioimmunoassay of angiotensin in human plasma. Nature (Lond.) 215, 714 (1967).

    Article  CAS  Google Scholar 

  • Waldhausl, W. K., Lucas, C. P., Conn, J. W., Lutz, J. H., Cohen, E. L.: Studies on the partial isolation of human renin. Biochim. biophys. Acta (Amst.) 221, 536 (1970).

    CAS  Google Scholar 

  • Wisenbaugh, P. E., Wills, N. E., Hill, R. W.: Paper chromatographic identification of angiotensins. Amer. J. Physiol. 207, 759 (1964)

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. R. Boucher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Genest
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Cleveland Clinic Foundation, 44106, Cleveland, OH, USA

    Irvine H. Page M.D. (Emeritus Director of Research) (Emeritus Director of Research)

  2. Division of Research, The Cleveland Clinic Foundation, 44106, Cleveland, OH, USA

    F. Merlin Bumpus Ph.D. (Chairman) (Chairman)

Rights and permissions

Reprints and permissions

Copyright information

© 1974 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Boucher, R., Genest, J. (1974). Measurement of Renin and of Angiotensin (Extraction and Bioassay). In: Page, I.H., Bumpus, F.M. (eds) Angiotensin. Handbuch der experimentellen Pharmakologie / Handbook of Experimental Pharmacology, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65600-2_10

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-65600-2_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65602-6

  • Online ISBN: 978-3-642-65600-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation