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Urinary Kinins and Colostrokinin

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Bradykinin, Kallidin and Kallikrein

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Abstract

Werle, after the discovery of kallidin, looked for sources of the peptide other than blood, but he could not detect the peptide in human urine (Frey et al., 1950; Werle and Berek, 1950). Somewhat later Werle and Erdös (1953, 1954) studied the excretion of oxytocin in human urine. They extracted a material from urine that was a peptide, but in various tests it behaved differently from the posterior pituitary hormone. The agent, called substance Z, was adsorbed from pooled urine on zinc ferrocyanide and subsequently eluted with alkaline ethanol. After the removal of some of the impurities, substance Z was precipitated with sodium tetraphenylborate. Finally, the peptide was extracted with 96% methanol, dried in vacuum, and dissolved in water. Injection of substance Z intravenously lowered the blood pressure of various laboratory animals. Intraperitoneal injection of the preparation had an antidiuretic effect in the rat. In vitro the peptide contracted isolated smooth muscles. Some subtle differences such as stability in alkali or lack of action on the rat colon seemed to distinguish this material from kallidin. Both of these properties of substance Z now can be attributed to the presence of impurities in the crude preparations. The peptide was easily destroyed by blood plasma, hemolyzed erythrocytes, chymotrypsin, etc. The inactivation of the material in serum was blocked by cysteine (Buchner, 1955). Substanze Z was absent from rat urine.

Some of the experiments described here were supported by Grant HE 08764 from the National Institutes of Health, United States Public Health Service.

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  1. Ervin G. Erdös
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  1. Department of Pharmacology, University of Oklahoma Medical Center, Oklahoma City, Oklahoma, USA

    Ervin G. Erdös

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Erdös, E.G. (1970). Urinary Kinins and Colostrokinin. In: Erdös, E.G., Wilde, A.F. (eds) Bradykinin, Kallidin and Kallikrein. Handbook of Experimental Pharmacology / Handbuch der experimentellen Pharmakologie, vol 25 / 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46222-1_28

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  • DOI: https://doi.org/10.1007/978-3-642-46222-1_28

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-46224-5

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