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Methods for the Isolation and Investigation of the Diversity of Cold-Adapted Yeasts and Their Ex Situ Preservation in Worldwide Collections

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Cold-adapted Yeasts

Abstract

Cold-adapted (psychrophilic and psychrotolerant) yeasts have been isolated from a variety of substrates, using a variety of cultivation methods. Yeasts able to grow at as low as 0 °C have been isolated from cold substrates such as glaciers, snow, and deep-sea sediment, but also from temperate and tropical climates. A broad diversity of media and culture conditions have been used to isolate and cultivate these yeasts. Low-temperature incubation is used to select for psychrophiles, thus depending on the strains relatively long incubation time (up to 14 weeks) may be required. Cold-adapted yeast strains belong to many species in many clades of Ascomycota and Basidiomycota. Numerous strains have been deposited in public culture collections. Online strain catalogs of some public yeast culture collections include searchable fields for growth temperatures, allowing selection of yeasts able to grow at desired temperatures. Culture-independent methods for profiling yeast diversity in mixed communities can be used to profile populations, allowing detection of yeasts whose DNA is present in a specimen but that were not cultivated.

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Authors and Affiliations

  1. Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California Davis, Davis, CA, 95616, USA

    Kyria L. Boundy-Mills

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  1. Kyria L. Boundy-Mills
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Correspondence to Kyria L. Boundy-Mills .

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Editors and Affiliations

  1. Department of Agricultural, Environmental and Food Sciences, University of Perugia, Industrial Yeasts Collection DBVPG, Perugia, Italy

    Pietro Buzzini

  2. University of Innsbruck, Institute of Microbiology, Innsbruck, Austria

    Rosa Margesin

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Boundy-Mills, K.L. (2014). Methods for the Isolation and Investigation of the Diversity of Cold-Adapted Yeasts and Their Ex Situ Preservation in Worldwide Collections. In: Buzzini, P., Margesin, R. (eds) Cold-adapted Yeasts. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-39681-6_2

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