Skip to main content
SpringerLink
Log in

Tenogenic Differentiation of Ovine Amniotic Stem Cells Co-Cultured with Tenocytes

  • Chapter
  • First Online:
Trends in Veterinary Sciences

  • 1024 Accesses

Abstract

Tendons are constantly exposed to mechanical loads, particularly to injuries in sportsmen, workers, and elderly people. Spontaneous healing, slow and incomplete, often results in scar formation. An effective treatment that is able to stimulate complete tendon healing remains to be developed. The introduction of stem cells that are able to differentiate into mature tenocytes represents a promising strategy for tendon repair. The aim of the present research was to assess whether co-culture with ovine or equine tenocytes can stimulate well-characterized ovine amniotic epithelial stem cells (AESCs) to differentiate into tenocytes. Both co-cultures reprogrammed ovine AESCs toward a tenogenic lineage as shown by the acquisition of a fusiform shape with a flat nucleus, by the secretion of collagen type I, and by the expression of tendon—related genes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Farng E, Urdaneta AR, Barba D, Esmende S, McAllister DR (2008) The effects of GDF-5 and uniaxial strain on mesenchymal stem cells in 3-D culture. Clin Orthop Relat Res 466:1930–1937

    Article  PubMed  Google Scholar 

  • Harris MT, Butler DL, Boivin GP, Florer JB, Schantz EJ, Wenstrup RJ (2004) Mesenchymal stem cells used for rabbit tendon repair can form ectopic bone and express alkaline phosphatase activity in constructs. J Orthop Res 22:998–1003

    Article  PubMed  CAS  Google Scholar 

  • Insausti CL, Blanquer M, Bleda P, Iniesta P, Majado MJ, Castellanos G, Moraleda JM (2010) The amniotic membrane as a source of stem cells. Histol Histopathol 25:91–98

    PubMed  CAS  Google Scholar 

  • Liu W, Chen B, Deng D, Xu F, Cui L, Cao Y (2006) Repair of tendon defect with dermal fibroblast engineered tendon in a porcine model. Tissue Eng 12:775–788

    Article  PubMed  Google Scholar 

  • Longo UG, Lamberti A, Maffulli N, Denaro V (2010) Tissue engineered biological augmentation for tendon healing: a systematic review. Brit Med Bull 98:31–59

    Article  PubMed  Google Scholar 

  • Luo Q, Song G, Song Y, Xu B, Qin J, Shi Y (2009) Indirect co-culture with tenocytes promotes proliferation and mRNA expression of tendon/ligament related genes in rat bone marrow mesenchymal stem cells. Cytotechnology 61:1–10

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by a Tercas Foundation grant.

Author information

Authors and Affiliations

  1. Department of Biomedical Comparative Sciences, University of Teramo, Teramo, Italy

    O. Di Giacinto, E. Galiffa & A. Pomante

  2. Department of Biomedical Comparative Sciences, University of Teramo, StemTeCh Group, Teramo, Italy

    Valentina Curini, V. Russo, A. Mauro & B. Barboni

Authors
  1. Valentina Curini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Russo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Di Giacinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Mauro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Galiffa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Pomante
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. B. Barboni
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Valentina Curini .

Editor information

Editors and Affiliations

  1. , Dipartimento die Scienze Biopatologiche, UniversitĂ  degli Studi di Perugia, Perugia, 06100, Italy

    Cristiano Boiti

  2. , Dept. of Morphology, Biochemistry,, University of Messina, Messina, 98168, Italy

    Adriana Ferlazzo

  3. , Dipartimento di Patologia, Diagnostica, UniversitĂ  degli Studi di Perugia, Via San Costanzo 4, Perugia, 06126, Italy

    Alberto Gaiti

  4. , Dipartimento die SanitĂ  Pubblica Veterin, UniversitĂ  degli Studi di Messina, Messina, 98168, Italy

    Antonio Pugliese

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Curini, V. et al. (2013). Tenogenic Differentiation of Ovine Amniotic Stem Cells Co-Cultured with Tenocytes. In: Boiti, C., Ferlazzo, A., Gaiti, A., Pugliese, A. (eds) Trends in Veterinary Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-36488-4_9

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-36488-4_9

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-36487-7

  • Online ISBN: 978-3-642-36488-4

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation