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Gene Trap Mutagenesis

  • Chapter
Conditional Mutagenesis: An Approach to Disease Models

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 178))

Abstract

Our ability to genetically manipulate the mouse has had a great impact on medical research over the last few decades. Mouse genetics has developed into a powerful tool for dissecting the genetic causes of human disease and identifying potential targets for pharmaceutical intervention. With the recent sequencing of the human and mouse genomes, a large number of novel genes have been identified whose function in normal and disease physiology remains largely unknown. Government-sponsored multinational efforts are underway to analyze the function of all mouse genes through mutagenesis and phenotyping, making the mouse the interpreter of the human genome. A number of technologies are available for the generation of mutant mice, including gene targeting, gene trapping and transposon, chemical or radiation-induced mutagenesis. In this chapter, we review the current status of gene trapping technology, including its applicability to conditional mutagenesis.

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  1. Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX, 77381, USA

    A. Abuin, G. M. Hansen & B. Zambrowicz

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  1. A. Abuin
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  2. G. M. Hansen
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  3. B. Zambrowicz
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  1. Interfakultäres Institut für Biochemie (IFIB) Signaltransduktion — transgene Modelle, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076, Tübingen, Germany

    Robert Feil

  2. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1, rue Laurent Fries, 67404, Illkirch, France

    Daniel Metzger

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Abuin, A., Hansen, G.M., Zambrowicz, B. (2007). Gene Trap Mutagenesis. In: Feil, R., Metzger, D. (eds) Conditional Mutagenesis: An Approach to Disease Models. Handbook of Experimental Pharmacology, vol 178. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-35109-2_6

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