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Tetracycline-Controlled Genetic Switches

  • Chapter
Conditional Mutagenesis: An Approach to Disease Models

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 178))

Abstract

Unlike recombinase-mediated gene manipulations, tetracycline (Tet)-controlled genetic switches permit reversible control of gene expression in the mouse. Trancriptional activation can be induced by activators termed tTA (Tet-Off) or rtTA (Tet-On) in the absence and presence of Tet, respectively. The Tet-Off and Tet-On systems are complementary, and the decision to choose one over the other depends on the particular experimental strategy. Both systems were optimized over the years and can now be used to develop mouse models.

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Authors and Affiliations

  1. Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany

    R. Sprengel & M. T. Hasan

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  1. R. Sprengel
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  2. M. T. Hasan
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Editors and Affiliations

  1. Interfakultäres Institut für Biochemie (IFIB) Signaltransduktion — transgene Modelle, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076, Tübingen, Germany

    Robert Feil

  2. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1, rue Laurent Fries, 67404, Illkirch, France

    Daniel Metzger

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Sprengel, R., Hasan, M.T. (2007). Tetracycline-Controlled Genetic Switches. In: Feil, R., Metzger, D. (eds) Conditional Mutagenesis: An Approach to Disease Models. Handbook of Experimental Pharmacology, vol 178. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-35109-2_3

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