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Mitochondrial RNA Editing and Processing in Diplonemid Protists

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RNA Metabolism in Mitochondria

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 34))

Abstract

RNA editing and processing in the mitochondrion of Diplonema papillatum and other diplonemids are arguably the most complex processes of their kind described in any organelle so far. Prior to translation, each transcript has to be accurately trans-spliced from gene fragments encoded on different circular chromosomes. About half of the transcripts are massively edited by several types of substitution editing and addition of blocks of uridines. Comparative analysis of mitochondrial RNA processing among the three euglenozoan groups, diplonemids, kinetoplastids, and euglenids, highlights major differences between these lineages. Diplonemids remain poorly studied, yet they were recently shown to be extremely diverse and abundant in the ocean and hence are rapidly attracting increasing attention. It is therefore important to turn them into genetically tractable organisms, and we report here that they indeed have the potential to become such.

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Acknowledgments

We thank Daria Tashyreva, Galina Prokopchuk, and Anzhelika Butenko (Institute of Parasitology) for sharing unpublished data. Support from the Grant Agency of University of South Bohemia (050/2016/P to BK), the Czech Grant Agency (15-21974S and 16-18699S to JL), the ERC CZ (LL1601 to JL), the Canadian Institutes of Health Research (CIHR, MOP 70309 to GB), and from the Gordon and Betty Moore Foundation (GBMF4983.01 to GB and JL) is kindly acknowledged.

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Authors and Affiliations

  1. Institute of Parasitology, Biology Centre and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic

    Drahomíra Faktorová, Binnypreet Kaur & Julius Lukeš

  2. Department of Biochemistry and Robert-Cedergren, Centre for Bioinformatics and Genomics, Université de Montréal, Montreal, Canada

    Matus Valach & Gertraud Burger

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  1. Drahomíra Faktorová
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  2. Matus Valach
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  3. Binnypreet Kaur
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  4. Gertraud Burger
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  5. Julius Lukeš
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Correspondence to Julius Lukeš .

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  1. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA

    Jorge Cruz-Reyes

  2. Department of Biochemistry and Molecular Biology, Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada

    Michael W. Gray

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Faktorová, D., Valach, M., Kaur, B., Burger, G., Lukeš, J. (2018). Mitochondrial RNA Editing and Processing in Diplonemid Protists. In: Cruz-Reyes, J., Gray, M. (eds) RNA Metabolism in Mitochondria. Nucleic Acids and Molecular Biology, vol 34. Springer, Cham. https://doi.org/10.1007/978-3-319-78190-7_6

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