Abstract
Monocyclic aromatic hydrocarbons (MAHs) are being used as individual chemicals or as in mixtures of two or more chemicals in several industrial and household processes across the world. Among MAHs, the most common chemicals are benzene, toluene and xylene, and they are also known as volatile organic compounds (VOCs), among them benzene categorised as highly toxic chemical and also listed as human carcinogen. Benzene, toluene and xylene cause cytotoxicity to a nontarget organism-like Drosophila melanogaster as an individual (benzene or toluene or xylene)/in mixture (benzene-toluene-xylene or benzene-toluene or benzene-xylene). In this chapter, several cellular, biochemical and molecular approaches were used to evaluate cellular toxicity due to MAHs like benzene, toluene and xylene using Drosophila melanogaster as an alternative to animal. We also judged variable cytotoxicity patterns of MAHs when they are exposed individually or in a mixture of two/three chemicals. An antagonistic effect of xylene and toluene on benzene toxicity and additive/synergistic effect of xylene on toluene-induced toxicity were evident in Drosophila. This study shows that co-exposure of benzene-toluene-xylene causes reduced cellular and organismal toxicity as compared to individual test chemical on Drosophila melanogaster.
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References
Adami G, Larese F, Venier M, Barbieri P, LoCoco F, Reisenhofer E (2006a) Penetration of benzene, toluene and xylenes contained in gasoline’s through human abdominal skin in vitro. Toxicol In Vitro 20:1321e30
Adami G, Larese F, Venier M, Barbieri P, Lo Coco F, Reisenhofer E (2006b) Penetration of benzene, toluene and xylenes contained in gasolines through human abdominal skin in vitro. Toxicol In Vitro 20:1321–1330
Ahmed HH, Metwally FM, Rashad HM (2009) Toxicity of solvents exposure on the neuroendocrine system in rats: role of amino acids supplementation. Toxic Solvents Rep Opin 1:66e83
Al-Ghamdi SS, Raftery MJ, Yaqoob MM (2004) Toluene and p-xylene induced LLCPK1 apoptosis. Drug Chem Toxicol 27:425–432
Andrew LS, Lee EW, Witmer CM, Kocsis JJ, Snyder R (1977) Effects of toluene on the metabolism, disposition and hemopoietic toxicity of [3H] benzene. Biochem Pharmacol 26:293–300
Atkinson TJ (2009) A review of the role of benzene metabolites and mechanisms in malignant transformation: summative evidence for a lack of research in nonmyelogenous cancer types. Int J Hyg Environ Health 212:1–10
Ayalogu OE, Igboh NM, Dede EB (2001) Biochemical changes in the serum and liver of albino rats exposed to petroleum samples (gasoline, kerosene, and crude petroleum). J Appl Sci Environ Manage 5:97e100
Badham HJ, Winn LM (2010) In utero exposure to benzene disrupts fetal hematopoietic progenitor cell growth via reactive oxygen species. Toxicol Sci 113:207–215
Barreto G, Madureira D, Capani F, Aon-Bertolino L, Saraceno E, Alvarez-Giraldez LD (2009) The role of catechols and free radicals in benzene toxicity: an oxidative DNA damage pathway. Environ Mol Mutagen 50:771–780
Benson JM, Gigliotti AP, March TH, Barr EB, Tibbetts BM, Skipper BJ, Clark CR, Twerdok L (2011) Chronic carcinogenicity of gasoline vapour condensate (GVC) and GVC containing methyl tertiary-butyl ether in f344 rats. J Toxicol Environ Health A 74:638e57
Brautbar N, Wu MP, Gabel E, Regev L (2006) Occupational kidney cancer exposure to industrial solvents. Ann N Y Acad Sci 1076:753e64
Bruckner JV, Warren DA (2001) Toxic effects of solvents and vapors. In: Klaassen CD (ed) Casarette and Doulls toxicology the basic science of poisons, 6th edn. McGraw-Hill Medical, New York, p 869e944
Chang FK, Chen ML, Cheng SF, Shih TS, Mao IF (2007) Dermal absorption of solvents as a major source of exposure among shipyard spray painters. J Occup Environ Med 49:430–436
Chapman DE, Namkung MJ, Juchau MR (1994) Benzene and benzene metabolites as embryotoxic agents: effects on cultured rat embryos. Toxicol Appl Pharmacol 128:129–137
Chen Z, Liu SJ, Cai SX, Yao YM, Yin H, Ukai H, Uchida Y, Nakatsuka H, Watanabe T, Ikeda M (1994) Exposure of workers to a mixture of toluene and xylenes. II. Effects. Occup Environ Med 51:47–59
Chen Y, McMillan-Ward E, Kong J, Israels SJ, Gibson SB (2008) Oxidative stress induces autophagic cell death independent of apoptosis in transformed and cancer cells. Cell Death Differ 15:171–182
Chou CC, Riviere JE, Monteiro-Riviere NA (2003) The cytotoxicity of jet fuel aromatic hydrocarbons and dose-related interleukin-8 release from human epidermal keratinocytes. Arch Toxicol 77:384–391
Croute F, Poinsot J, Gaubin Y, Beau B, Simon V, Murat JC, Soleilhavoup JP (2002) Volatile organic compounds cytotoxicity and expression of HSP72, HSP90 and GRP78 stress proteins in cultured human cells. Biochim Biophys Acta 1591:147–155
Das S, Chakrabarty D, Choudhury GC (2010) 1,4-benzoquinone (PBQ) induced toxicity in lung epithelial cells is mediated by the disruption of the microtubule network and activation of caspase-3. Chem Res Toxicol 23:1054–1066
Deng Z, Liu YD (2010) Epigenetic alterations as cancer diagnostic, prognostic, and predictive biomarkers. Adv Genet 71:125–176
Derwent RG, Davies TJ, Delaney M, Dollard GJ, Field RA, Dumitrean P, Nason PD, Jones BMR, Pepler SA (2000) Analysis and interpretation of the continuous hourly monitoring data for 26 C2–C8 hydrocarbons at 12 United Kingdom sites during 1996. Atmos Environ 34:297–312
Dogru O, Celkan T, Demir T (2007) Hematological and biochemical changes in volatile substance abusing street children in Istanbul. Turk J Hematol 24:52e6
Dosemeci M, Li GL, Hayes RB, Yin SN, Linet M, Chow WH, Wang YZ, Jiang ZL, Dai TR, Zhang WU (1994) Cohort study among workers exposed to benzene in China: II. Exposure assessment. Am J Ind Med 26:401–411
Erexson GL, Wilmer JL, Steinhagen WH, Kligerman AD (1986) Induction of cytogenetic damage in rodents after short-term inhalation of benzene. Environ Mutagen 8:29–40
Faiola B, Fuller ES, Wong VA, Pluta L, Abernethy DJ, Rose J, Recio L (2004) Exposure of hematopoietic stem cells to benzene or 1,4-benzoquinone induces gender-specific gene expression. Stem Cells 22:750–758
Gad-El-Karim MM, Harper BL, Legator MS (1984) Modifications in the myeloclastogenic effect of benzene in mice with toluene, phenobarbital, 3- methylcholanthrene, Aroclor 1254, SKF-525A. Mutat Res 135:225–243
Gallegos P, Lutz J, Markwiese J, Ryti R, Mirenda R (2007) Wildlife ecological screening levels for inhalation of volatile organic chemicals. Environ Toxicol Chem 26:1299–1303
Galvan N, Lim S, Zmugg S, Smith MT, Zhang L (2008) Depletion of WRN enhances DNA damage in HeLa cells exposed to the benzene metabolite, hydroquinone. Mutat Res 649:54–61
Gayathri MV, Krishnamurthy NB (1981) Studies on the toxicity of mercurial fungicide Agallol3 in Drosophila melanogaster. Environ Res 24:89–95
Greer JE (1984) Adolescent abuse of typewriter correction fluid. South Med J 77:297–298
Hay BA, Guo M (2006) Caspase-dependent cell death in Drosophila. Annu Rev Cell Dev Biol 22:623–650
Hu J, Ma H, Zhang W, Yu Z, Fu J (2014) Effects of benzene and its metabolites on global DNA methylation in human normal hepatic L02 cells. Environ Toxicol 29:108–116
Irons RD, Stillman WS, Colagiovanni DB, Henry VA (1992) Synergistic action of benzene metabolite hydroquinone on myelopoietic stimulating activity of granulocyte/macrophage colony-stimulating in vitro. Proc Natl Acad Sci U S A 89:3691–3695
Irons RD, Chen Y, Wang X, Ryder J, Kerzic PJ (2013) Acute myeloid leukemia following exposure to benzene more closely resembles de novo than therapy related-disease. Genes Chromosomes Cancer 52:887–894
Kim KH, Kim MY (2002) The distributions of BTEX compounds in the ambient atmosphere of the Nan-Ji-Do abandoned landfill site in Seoul. Atmos Environ 36:2433–2446
Kolachana P, Subrahmanyam VV, Meyer K, Zhang L, Smith M (1993) Benzene and its phenolic metabolites produce oxidative DNA damage in HL-60 cells in vitro and in the bone marrow in vivo. Cancer Res 53:1023–1026
Kozel N, Sloboda Z, De La Rosa M (eds) (1995) Epidemiology of inhalant abuse: an international perspective. US Department of Health and Human Services, Washington, DC. NIDA Res. Mon. 14
Kuang S, Liang W (2005) Clinical analysis of 43 cases of chronic benzene poisoning. Chem Biol Interact 30:129–135
Lagorio S, Ferrante D, Ranucci A, Negri S, Sacco P, Rondelli R, Cannizzaro S, Torregrossa MV, Cocco P, Forastiere F, Miligi L, Bisanti L, Magnani C (2013) Exposure to benzene and childhood leukaemia: a pilot case-control study. BMJ Open 3:e002275
Lee SC, Chiu MY, Ho KF, Zou SC, Wang X (2002) Volatile organic compounds (VOCs) in urban atmosphere of Hong Kong. Chemosphere 48:375–382
Li G, Yin S (2006) Progress of epidemiological and molecular epidemiological studies on benzene in China. Ann N Y Acad Sci 1076:800–809
Lippmann SJ, Richardson DB, Chen JCM (2011) Elevated serum liver enzymes and fatty liver changes associated with long driving among taxi drivers. Am J Ind Med 54:618e27
Medinsky MA, Schlosser PM, Bond JA (1994) Critical issues in benzene toxicity and metabolism: the effect of interactions with other organic chemicals on risk assessment. Environ Health Perspect 102:119–124
Medinsky MA, Kenyon EM, Seaton MJ, Schlosser PM (1996) Mechanistic considerations in benzene physiological model development. Environ Health Perspect 104:1399–1404
Nakai N, Murata M, Nagahama M, Hirase T, Tanaka M, Fujikawa T, Nakao N, Nakashima K, Kawanishi S (2003) Oxidative DNA damage induced by toluene is involved in its male reproductive toxicity. Free Radic Res 37:69–76
Perigo JF, Prado C (2005) Evolution of occupational exposure to environmental levels of aromatic hydrocarbons in service stations. Ann Occup Hyg 49(233e):40
Plappert U, Barthel E, Seidel HJ (1994) Reduction of benzene toxicity by toluene. Environ Mol Mutagen 24:283–292
Rappaport SM, Kim S, Thomas R, Johnson BA, Bois FY, Kupper LL (2013) Low-dose metabolism of benzene in humans: science and obfuscation. Carcinogenesis 34:2–9
Revilla AS, Pestana CR, Pardo-Andreu GL, Santos AC, Uyemura SA, Gonzales ME (2007) Potential toxicity of toluene and xylene evoked by mitochondrial uncoupling. Toxicol In Vitro 21:782e8
Ross D (2000) The role of metabolism and specific metabolites in benzene-induced toxicity: evidence and issues. J Toxicol Environ Health A 61:357–372
Saadat M, Ansari-Lari M (2005) Alterations of liver function test indices of filling station workers with respect of genetic polymorphisms of GSTM1 and GSTT1. Cancer Lett 227:163e7
Siddique HR, Kar Chowdhuri D, Saxena DK, Dhawan A (2005) Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay. Mutagenesis 4:285–290
Singh R, Winn LM (2008) The effects of 1,4-benzoquinone on c-Myb and topoisomerase II in K-562 cells. Mutat Res 645:33–38
Singh MP, Reddy MM, Mathur N, Saxena DK, Chowdhuri DK (2009) Induction of hsp70, hsp60, hsp83 and hsp26 and oxidative stress markers in benzene, toluene and xylene exposed Drosophila melanogaster: role of ROS generation. Toxicol Appl Pharmacol 235(2):226–243
Singh MP, Ram KR, Mishra M, Shrivastava M, Saxena DK, Chowdhuri DK (2010) Effects of co-exposure of benzene, toluene and xylene to Drosophila melanogaster: alteration in hsp70, hsp60, hsp83, hsp26, ROS generation and oxidative stress markers. Chemosphere 79(5):577–587
Singh MP, Mishra M, Sharma A, Shukla AK, Mudiam MK, Patel DK, Ram KR, Chowdhuri DK (2011) Genotoxicity and apoptosis in Drosophila melanogaster exposed to benzene, toluene and xylene: attenuation by quercetin and curcumin. Toxicol Appl Pharmacol 253(1):14–30
Smith MT (1996) Mechanistic studies of benzene toxicity—implications for risk assessment. Adv Exp Med Biol 387:259–266
Smith MT, Yager JW, Steinmetz KL, Eastmond DA (1989) Peroxidase-dependent metabolism of benzene’s phenolic metabolites and its potential role in benzene toxicity and carcinogenicity. Environ Health Perspect 82:23–29
Snyder R (2000) Overview of the toxicology of benzene. J Toxicol Environ Health A 61:339–346
Snyder R (2007) Benzene’s toxicity: a consolidated short review of human and animal studies by HA Khan. Hum Exp Toxicol 26:687–696
Snyder R (2012) Leukemia and benzene. Int J Environ Res Public Health 9:2875–2893
Spiller HA, Krenzelok EP (1997) Epidemiology of inhalant abuse reported to two regional poison centers. J Toxicol Clin Toxicol 35:167–173
Srivastava A, Devotta S (2007) Indoor air quality of public places in Mumbai, India in terms of volatile organic compounds. Environ Monit Assess 133:127–138
Stokes SE, Winn LM (2014) NF-kappaB signaling is increased in hd3 cells following exposure to 1,4-benzoquinone: role of reactive oxygen species and p38-MAPK. Toxicol Sci 137:303–310
Sul D, Lee E, Lee MY, Oh E, Im H, Lee J, Jung WW, Won N, Kang HS, Kim EM, Kang SK (2005) DNA damage in lymphocytes of benzene exposed workers correlates with trans, trans-muconic acids and breath benzene levels. Mutat Res 582:61–70
Tian JF, Peng CH, Yu XY, Yang XJ, Yan HT (2012) Expression and methylation analysis of p15 and p16 in mouse bone marrow cells exposed to 1,4-benzoquinone. Hum Exp Toxicol 31:718–725
US EPA 1996 Priority pollutants, code of federal regulations. Title 40; U.S. Environmental Protection Agency. U.S. Government Printing Office, Washington, DC. Part 423, App. A (chapter 1)
Wan J, Winn LM (2004) The effects of benzene and the metabolites phenol and catechol on c-Myb and Pim-1 signaling in HD3 cells. Toxicol Appl Pharmacol 201:194–201
Wang L, Zhou Y, Liang Y, Wong O, Armstrong T, Schnatter AR, Wu Q, Fang J, Ye X, Fu H, Irons RD (2006) Benzene exposure in the shoemaking industry in China, a literature survey, 1978–2004. Regul Toxicol Pharmacol 46:149–156
Weaver CV, Liu SP (2008) Differentially expressed pro- and anti-apoptogenic genes in response to benzene exposure: immunohistochemical localization of p53, Bag, Bad, Bax, Bcl-2, and Bcl-w in lung epithelia. Exp Toxicol Pathol 59:265–272
Weaver CV, Liu SP, Lu JF, Lin BS (2007) The effects of benzene exposure on apoptosisin epithelial lung cells: localization by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and the immunocytochemical localization of apoptosis-related gene products. Cell Biol Toxicol 23:201–220
Weisel CP (2010) Benzene exposure: an overview of monitoring methods and their findings. Chem Biol Interact 184:58–66
Wetmore BA, Struve MF, Gao P, Sharma S, Allison N, Roberts KC, Letinski DJ, Nicolich MJ, Bird MG, Dorman DC (2008) Genotoxicity of intermittent co-exposure to benzene and toluene in male CD-1 mice. Chem Biol Interact 173:166–178
Wong O, Raabe GK (1989) Critical review of cancer epidemiology in petroleum industry employees, with a quantitative meta-analysis by cancer site. Am J Ind Med 15:283–310
Xing Q, Chen G, Li L, Zhang M, Zheng Z, Zou L, Hou QF, Wang X, Liu XG (2013) Microsomal epoxide hydrolase (EPHX1) polymorphisms are associated with aberrant promoter methylation of ERCC3 and hematotoxicity in benzene-exposed workers. Environ Mol Mutagen 54:397–405
Yang F, Zhou JH (2010) Cytotoxicity and DNA damage induced by 1,4-benzoquinone in v79 Chinese hamster lung cells. J Toxicol Environ Health A 73:483–489
Zhang L, Eastmond DA, Smith MT (2002) The nature of chromosomal aberrations detected in humans exposed to benzene. Crit Rev Toxicol 32:1–42
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Singh, M.P., Himalian, R. (2018). Monocyclic Aromatic Hydrocarbons (MAHs) Induced Toxicity in Drosophila: How Close How Far?. In: Kumar, D., Gong, C. (eds) Trends in Insect Molecular Biology and Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-319-61343-7_3
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