Abstract
Metabolic reprogramming is a required step during oncogenesis and essential for cellular proliferation. It is triggered by activation of oncogenes and loss of tumor suppressor genes. Beside the combinatorial events leading to cancer, common changes within the central metabolism are reported. Increase of glycolysis and subsequent lactic acid formation has been a focus of cancer metabolism research for almost a century. With the improvements of bioanalytical techniques within the last decades, a more detailed analysis of metabolism is possible and recent studies demonstrate a wide range of metabolic rearrangements in various cancer types. However, a systematic and mechanistic understanding is missing thus far. Therefore, analytical and computational tools have to be developed allowing for a dynamic and quantitative analysis of cancer metabolism. In this chapter, we outline the application of pulsed stable isotope resolved metabolomics (pSIRM) and describe the interface toward computational analysis of metabolism.
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Abbreviations
- CCM:
-
Central carbon metabolism
- DDA:
-
Data-dependent analysis
- FBA:
-
Flux balance analysis
- FBP:
-
Fructose-1,6-bisphosphate
- FGFR1:
-
Fibroblast growth factor receptor 1
- GC-MS:
-
Gas-chromatography–mass spectrometry
- Glc:
-
Glucose
- HIF-1:
-
Hypoxia-inducible factor 1
- ICAT:
-
Isotopic-coded affinity tag
- ID-MS:
-
Isotope dilution mass spectrometry
- INST-MFA:
-
Nonstationary metabolic flux analysis
- iTRAQ:
-
Isobaric tags for relative and absolute quantification
- Lac:
-
Lactic acid
- LC-MS:
-
Liquid chromatography–mass spectrometry
- MFA:
-
Metabolic flux analysis
- MID:
-
Mass isotopomer distribution
- MS:
-
Mass spectrometry
- N:
-
Stoichiometric matrix
- NMR:
-
Nuclear magnetic resonance
- Oct-4:
-
Octamer-binding transcription factor 4
- PEP:
-
Phosphoenolpyruvic acid
- PK:
-
Pyruvate kinase
- PKM1/PKM2:
-
Pyruvate kinase isoform M1/isoform M2
- PPP:
-
Pentose phosphate pathway
- pSIRM:
-
Pulsed stable isotope resolved metabolomics
- Pyr:
-
Pyruvic acid
- ROS:
-
Reactive oxygen species
- SILAC:
-
Stable isotope labeling of amino acids in cell culture
- STAT3:
-
Signal transducers and activators of transcription
- TCA cycle:
-
Tricarboxylic acid cycle
- TIS:
-
Therapy-induced senescence
- TMFA:
-
Thermodynamics-based metabolic flux analysis
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Zasada, C., Kempa, S. (2016). Quantitative Analysis of Cancer Metabolism: From pSIRM to MFA. In: Cramer, T., A. Schmitt, C. (eds) Metabolism in Cancer. Recent Results in Cancer Research, vol 207. Springer, Cham. https://doi.org/10.1007/978-3-319-42118-6_9
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