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Isolation and Characterization of Stem Cells from Human Central Nervous System Malignancies

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Stem Cell Biology in Neoplasms of the Central Nervous System

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 853))

Abstract

Central Nervous System (CNS) tumors include some of the most invasive and lethal tumors in humans. The poor prognosis in patients with CNS tumors is ascribed to their invasive nature. After the description of a stem cell-like cohort in hematopoietic cancers, tumor stem cells (TSCs) have been isolated from a variety of solid tumors, including brain tumors. Further research has uncovered the crucial role these cells play in the initiation and propagation of brain tumors. More importantly, TSCs have also been shown to be relatively resistant to conventional cytotoxic therapeutics, which may also account for the alarmingly high rate of CNS tumor recurrence. In order to elucidate prospective therapeutic targets it is imperative to study these cells in detail and to accomplish this, we need to be able to reliably isolate and characterize these cells. This chapter will therefore, provide an overview of the methods used to isolate and characterize stem cells from human CNS malignancies.

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Authors and Affiliations

  1. Section of Neurosurgery, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA

    Imad Saeed Khan M.D.

  2. Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA

    Moneeb Ehtesham M.D.

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  1. Department of Neurosurgery, Vanderbilt University Medical Center, Nashville, Tennessee, USA

    Moneeb Ehtesham

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Khan, I.S., Ehtesham, M. (2015). Isolation and Characterization of Stem Cells from Human Central Nervous System Malignancies. In: Ehtesham, M. (eds) Stem Cell Biology in Neoplasms of the Central Nervous System. Advances in Experimental Medicine and Biology, vol 853. Springer, Cham. https://doi.org/10.1007/978-3-319-16537-0_3

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