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X-ray crystallographic studies of mammalian carbonic anhydrase isozymes

  • Chapter
The Carbonic Anhydrases

Part of the book series: EXS 90 ((EXS,volume 90))

Abstract

The year 1997 marked the 25th year of x-ray crystallographic studies of the mammalian carbonic anhydrase (CA) isozymes. These remarkable enzymes catalyze the reversible hydration of carbon dioxide (H2O + CO2 ⇔ HCO– 3 + H+) via a zinc-hydroxide mechanism (Coleman, 1986; Silverman and Lindskog, 1988; Christianson and Fierke, 1996). To date, five of the seven known isozymes have yielded x-ray crystal structures: three cytosolic isozymes, human CAI (Kannan et al., 1984), human CA II ( Liljas et al., 1972; Eriksson et al., 1988; Halansson, 19929, and bovine CA III (Eriksson and Liljas, 1993); a membrane-associated isozyme, human CA IV (Stams et al., 1996); and a mitochondrial isozyme, murine CA V (Boriack-Sjodin et al., 1995). X-ray crystallographic studies of cytosolic isozymes I, II, and III have been previously reviewed by Eriksson and Liljas (1991). In this review, we extend structural comparisons within the CA family to include the membrane-associated isozyme IV and the mitochondrial isozyme V. We note that to date, the structures of two mammalian CA isozymes remain undetermined: CA VI, a secretory protein found in saliva, and CA VII, a cytosolic isozyme found primarily in salivary glands.

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Authors and Affiliations

  1. Department of Chemistry, University of Pennsylvania, Philadelphia, PA, 19104-6323, USA

    Travis Stams & David W. Christianson

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  1. Travis Stams
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  2. David W. Christianson
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Editors and Affiliations

  1. Lake Erie College of Osteopathic Medicine, 1858 West Grandview Boulevard, Erie, PA, 16509, USA

    W. Richard Chegwidden

  2. Medical Genetics Unit, St George’s Hospital Medical School, London, SW17 0RE, UK

    Nicholas D. Carter

  3. MRC Human Biochemical Genetics Unit, Wolfson House, University College London, 4, Stephenson Way, London, NW1 2HE, UK

    Yvonne H. Edwards

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© 2000 Springer Basel AG

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Stams, T., Christianson, D.W. (2000). X-ray crystallographic studies of mammalian carbonic anhydrase isozymes. In: Chegwidden, W.R., Carter, N.D., Edwards, Y.H. (eds) The Carbonic Anhydrases. EXS 90, vol 90. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8446-4_9

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  • DOI: https://doi.org/10.1007/978-3-0348-8446-4_9

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9570-5

  • Online ISBN: 978-3-0348-8446-4

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