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Yeast Hsp70 and J-protein Chaperones: Function and Interaction Network

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The Molecular Chaperones Interaction Networks in Protein Folding and Degradation

Part of the book series: Interactomics and Systems Biology ((INTERACTOM,volume 1))

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Abstract

All heat shock protein (Hsp)70 molecular chaperones use the same fundamental biochemical mechanism of action, cycles of interaction with client proteins driven by adenosine triphosphate (ATP) binding and hydrolysis to maintain cellular proteostasis. However, they do not function alone; the action of co-chaperones is required. Together with Hsp70s, the J-proteins and nucleotide exchange factors (NEF) co-chaperones form Hsp70 networks. These networks are complex assembles with the number of components and their mutual interactions varying from species to species. Here, we consider Hsp70 networks functioning in a single cell eukaryote, baker’s yeast. By combining network theory perspective with available functional data and evolutionary analysis, we argue that it is possible to make sense of these complexities.

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Abbreviations

ER:

Endoplasmic reticulum

ERAD:

ER-associated degradation

IMS:

Intermembrane space domain

mtDNA:

Mitochondrial deoxyribonucleic acid (DNA)

mtHsp70:

Mitochondrial heat shock protein (Hsp)70

NBD:

Nucleotide-binding domain

NEF:

Nucleotide exchange factor

PDR:

Pleiotropic drug resistance

RAC:

Ribosome-associated complex

SBD:

Substrate-binding domain

WGD:

Whole genome duplication

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Acknowledgments

Work in the laboratory of E.A. Craig was supported by the National Institutes of Health grants GM31107 and GM27870 (E.A.C.); work in the laboratory of J. Marszalek was supported by Foundation for Polish Science grant TEAM/2009−3/5 and Polish National Science Center grant Maestro DEC-2012/06/A/NZ1/000

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Authors and Affiliations

  1. Department of Biochemistry, University of Wisconsin–Madison, Madison, WI, USA

    Elizabeth A. Craig PhD

  2. Laboratory of Evolutionary Biochemistry, Intercollegiate Faculty of Biotechnology, University of Gdansk, Gdansk, Poland

    Jaroslaw Marszalek PhD

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Correspondence to Elizabeth A. Craig PhD .

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Editors and Affiliations

  1. Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

    Walid A. Houry

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Craig, E., Marszalek, J. (2014). Yeast Hsp70 and J-protein Chaperones: Function and Interaction Network. In: Houry, W. (eds) The Molecular Chaperones Interaction Networks in Protein Folding and Degradation. Interactomics and Systems Biology, vol 1. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1130-1_3

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