Abstract
The next-generation sequencing methods that we described in Chap. 3 were implemented into several commercial instruments in order to make the sequencing technologies available to individual laboratories. In this chapter, we will overview the major DNA sequencing platforms and present several comparisons for their advantages, disadvantages, sequencing costs, and other aspects.
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References
Liu L, Li Y, Li S, Hu N, He Y et al. (2012) Comparison of next-generation sequencing systems. Journal of biomedicine & biotechnology 2012:251364. doi:10.1155/2012/251364
Quail MA, Smith M, Coupland P, Otto TD, Harris SR et al. (2012) A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics 13:341. doi:10.1186/1471-2164-13-341
Glenn TC (2011) Field guide to next-generation DNA sequencers. Mol Ecol Resour 11 (5):759-769. doi:10.1111/j.1755-0998.2011.03024.x
El-Metwally S, Hamza T, Zakaria M, Helmy M (2013) Next-generation sequence assembly: four stages of data processing and computational challenges. PLoS Comput Biol 9 (12):e1003345. doi:10.1371/journal.pcbi.1003345
Mardis ER (2008) The impact of next-generation sequencing technology on genetics. Trends Genet 24 (3):133-141. doi:10.1016/j.tig.2007.12.007
Huse SM, Huber JA, Morrison HG, Sogin ML, Welch DM (2007) Accuracy and quality of massively parallel DNA pyrosequencing. Genome Biol 8 (7):R143. doi: gb-2007-8-7-r143
454.com GS FLX+ Systems http://454.com/products/gs-flx-system/index.asp. Accessed 10-01-2014
Life Technologies Inc. (2012) 5500 W SerieS Genetic Analyzers. http://tools.lifetechnologies.com/content/sfs/brochures/5500-w-series-spec-sheet.pdf Accessed 10-01-2014
Illumina Inc. (2011) Genome Analyzer IIX. http://res.illumina.com/documents/products/datasheets/datasheet_genome_analyzeriix.pdf. Accessed 10-01-2014
Illumina Inc. (2014) Illumina Sequencing Systems. http://www.illumina.com/systems/sequencing.ilmn. Accessed 10-01-2014
Illumina Inc. (2014) HiSeq X Ten. http://www.illumina.com/systems/hiseq-x-sequencing-system.ilmn. Accessed 17-01-2014
Collins FS, Hamburg MA (2013) First FDA authorization for next-generation sequencer. N Engl J Med 369 (25):2369-2371. doi:10.1056/NEJMp1314561
Life Technologies Inc. (2014) Ion Personal Genome Machine. https://www.lifetechnologies.com/order/catalog/product/4462921. Accessed 10-01-2014
Mellmann A, Harmsen D, Cummings CA, Zentz EB, Leopold SR et al. (2011) Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology. PLoS One 6 (7):e22751. doi:10.1371/journal.pone.0022751
Rohde H, Qin J, Cui Y, Li D, Loman NJ et al. (2011) Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4. N Engl J Med 365 (8):718-724. doi:10.1056/NEJMoa1107643
Chin CS, Sorenson J, Harris JB, Robins WP, Charles RC et al. (2011) The origin of the Haitian cholera outbreak strain. N Engl J Med 364 (1):33-42. doi:10.1056/NEJMoa1012928
Schaaf CP, Gonzalez-Garay ML, Xia F, Potocki L, Gripp KW et al. (2013) Truncating mutations of MAGEL2 cause Prader-Willi phenotypes and autism. Nat Genet 45 (11):1405-1408. doi:10.1038/ng.2776
Glenn TC (2013) Field guide to next-generation DNA sequencers-Update. http://www.molecularecologist.com/next-gen-fieldguide-2013/. Accessed 10-01-2014
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El-Metwally, S., Ouda, O.M., Helmy, M. (2014). Next-Generation Sequencing Platforms. In: Next Generation Sequencing Technologies and Challenges in Sequence Assembly. SpringerBriefs in Systems Biology, vol 7. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0715-1_4
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DOI: https://doi.org/10.1007/978-1-4939-0715-1_4
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