Next Generation Sequencing Technologies and Challenges in Sequence Assembly

  • Sara El-Metwally
  • Osama M. Ouda
  • Mohamed Helmy

Part of the SpringerBriefs in Systems Biology book series (BRIEFSBIOSYS, volume 7)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction to Molecular Biology and Bioinformatics Basics in Next-Generation Sequencing

    1. Front Matter
      Pages 1-1
    2. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 3-13
    3. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 15-26
  3. Next-generation Sequencing Methods, Platforms, Applications and Challenges

    1. Front Matter
      Pages 27-27
    2. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 29-36
    3. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 37-44
    4. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 45-49
    5. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 51-59
    6. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 61-70
  4. Next-generation Sequence Assembly Stages, Assessments, Tools and Challenges

    1. Front Matter
      Pages 71-71
    2. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 73-78
    3. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 79-93
    4. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 95-101
    5. Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
      Pages 103-116
  5. Back Matter
    Pages 117-118

About this book

Introduction

The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field.

This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

Keywords

challenges genomics next generation sequence technologies next generation sequencing sequence assembly

Authors and affiliations

  • Sara El-Metwally
    • 1
  • Osama M. Ouda
    • 2
  • Mohamed Helmy
    • 3
  1. 1.Department of Computer ScienceMansoura UniversityMansouraEgypt
  2. 2.Department of Information TechnologyMansoura UniversityMansouraEgypt
  3. 3.Botany Department and Biotechnology Department, and The Donnelly Centre for Cellular and Biomolecular ResearchAl-Azhar University, and University of Toronto (UofT)CairoEgypt

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0715-1
  • Copyright Information The Authors 2014
  • Publisher Name Springer, New York, NY
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-1-4939-0714-4
  • Online ISBN 978-1-4939-0715-1
  • Series Print ISSN 2193-4746
  • Series Online ISSN 2193-4754
  • About this book
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