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Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins

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Membrane Proteins Production for Structural Analysis

Abstract

Membrane proteins (MPs) play key roles in most important cellular processes ranging from cell-to-cell communication to signaling processes. Despite recent improvements, the expression of functionally folded MPs in sufficient amounts for functional and structural characterization remains a challenge. Indeed, it is still difficult to predict whether a protein can be overproduced in a functional state in some expression system(s), although studies of high-throughput screens have been issued in recent years. Prokaryotic expression systems present several advantages over eukaryotic ones. Among them, Lactococcus lactis (L. lactis) has emerged in the past years as a good alternative expression system to Escherichia coli. The purpose of this chapter is to describe L. lactis and its tightly inducible system, nisin-controlled gene expression (NICE), for the expression of MPs from both prokaryotic and eukaryotic origins. Moreover, we will describe the functional characterization of some MPs produced in L. lactis.

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Acknowledgments

This work has been supported by the Region Ile de France (DIM SeNt, PhD fellowship to SB AAP2010-3-10T6). We thank Edmund Kunji for his kind help and critical reading of the chapter.

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Authors and Affiliations

  1. Oxidative Stress and Detoxification Laboratory, UMR-CNRS 8221, Institute of Biology and Technology of Saclay, SB2SM, and Centre for Nuclear Studies and Université Paris-Suds, 91191, Gif-sur-Yvette, France

    Sana Bakari, François André, Marcel Delaforge & Annie Frelet-Barrand

  2. Cell & Plant Physiology Laboratory, Institute of Sciences Research and Technologies, UMR-CNRS 5168, National Institute of Agronomical Research, Centre for Nuclear Studies, Université Grenoble Alpes, 91191, Grenoble, France

    Daphné Seigneurin-Berny & Norbert Rolland

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  5. Norbert Rolland
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  6. Annie Frelet-Barrand
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Correspondence to Annie Frelet-Barrand .

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  1. UMR-CNRS 7275, Institute of Molecular and Cellular Pharmacology, Valbonne, France

    Isabelle Mus-Veteau

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Bakari, S., André, F., Seigneurin-Berny, D., Delaforge, M., Rolland, N., Frelet-Barrand, A. (2014). Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins. In: Mus-Veteau, I. (eds) Membrane Proteins Production for Structural Analysis. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0662-8_5

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