Membrane Proteins Production for Structural Analysis

  • Isabelle Mus-Veteau

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. Isabelle Mus-Veteau, Pascal Demange, Francesca Zito
    Pages 1-44
  3. Davide Proverbio, Erik Henrich, Erika Orbán, Volker Dötsch, Frank Bernhard
    Pages 45-70
  4. Jean-Louis Banères
    Pages 71-86
  5. Georges Hattab, Annabelle Y. T. Suisse, Oana Ilioaia, Marina Casiraghi, Manuela Dezi, Xavier L. Warnet et al.
    Pages 87-106
  6. Sana Bakari, François André, Daphné Seigneurin-Berny, Marcel Delaforge, Norbert Rolland, Annie Frelet-Barrand
    Pages 107-132
  7. Manuela Zoonens, Francesca Zito, Karen L. Martinez, Jean-Luc Popot
    Pages 173-203
  8. Andrii Ishchenko, Enrique Abola, Vadim Cherezov
    Pages 289-314
  9. Jean-Jacques Lacapere, Soria Iatmanen-Harbi, Lucile Senicourt, Olivier Lequin, Piotr Tekely, Rudra N. Purusottam et al.
    Pages 393-421
  10. Back Matter
    Pages 423-425

About this book

Introduction

This volume reviews the latest development in production, stabilization and structural analysis techniques of membrane proteins. It contains 14 chapters exploring topics including the advances in heterologous expression systems, stabilization tools and structural methods that contributed to the growing number of recombinant integral membrane protein structures solved in the past few years. Each chapter was written by internationally renowned scientists in the field of membrane proteins structural characterization.

Membrane proteins account for roughly 30 percent of all open reading frames in fully sequenced genomes. However, to date, atomic structures have so far been obtained for only 424 integral membrane proteins, with 100 new structures determined in the last two years. Only 10 percent of the unique integral membrane protein structures are derived from vertebrates. In general, integral membrane proteins are present in tissues at very low concentration, making production of recombinant proteins in heterologous systems suitable for large scale production a prerequisite for structural studies. Since the first atomic structures of recombinant mammalian integral membrane proteins published in 2005 (the calcium ATPase SERCA 1A and a voltage-dependent potatium channel), the structures of 37 recombinant mammalian integral membrane proteins, from which 20 belong to the G Protein Coupled Receptors family, have been solved.

Keywords

amphipols liposomes mammalian membrane mitochondrial membrane pichia pastoris

Editors and affiliations

  • Isabelle Mus-Veteau
    • 1
  1. 1.UMR-CNRS 7275Institute of Molecular and Cellular PharmacologyValbonneFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0662-8
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Springer, New York, NY
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-1-4939-0661-1
  • Online ISBN 978-1-4939-0662-8
  • About this book
Industry Sectors
Biotechnology
Consumer Packaged Goods
Pharma