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Electrophysiology of Osteoclasts and Macrophages

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Macrophages and Related Cells

Part of the book series: Blood Cell Biochemistry ((BLBI,volume 5))

Abstract

Membrane potential and ionic conductances play a role in regulating many cell functions. In macrophages, it has been suggested that ionic currents participate in transmembrane signaling, phagocytosis, secretion, and motility (Ince et al., 1988; Riches et al., 1988; Gallin, 1991). Macrophages have been shown to possess several different types of K+ and Cl-channels. Osteoclasts, which are specialized cells responsible for resorption of mineralized tissues, have a number of different plasma membrane ion transporters, including a proton ATPase, as well as K+ and Cl-channels. These ion channels and transporters are thought to be involved in the process and regulation of H+ transport, and hence bone resorption (Vaes, 1988; Blair et al., 1989, 1991; Hall, 1991; Sims et al.,1992).

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References

  • Aderem, A. A., Scott, W. A., and Cohn, Z. A., 1984, A selective defect in arachidonic acid release from macrophage membranes in high potassium media, J. Cell Biol. 99: 1235–1241.

    Article  PubMed  CAS  Google Scholar 

  • Anderson, R. E., Woodbury, D. M., and Jee, W. S. S., 1986, Humoral and ionic regulation of osteoclast acidity, Calcif. Tissue Int. 39: 252–258.

    Article  PubMed  CAS  Google Scholar 

  • Arkett, S. A., Dixon, S. J., and Sims, S. M., 1992, Substrate influences rat osteoclast morphology and expression of potassium conductances, J. Physiol. (London) 458: 633–653.

    CAS  Google Scholar 

  • Arnett, T. R., and Dempster, D. W., 1990, Perspectives: Protons and osteoclasts, J. Bone Miner. Res. 5: 1099–1103.

    Article  PubMed  CAS  Google Scholar 

  • Baron, R., Neff, L., Louvard, D., and Courtoy, P. J., 1985, Cell-mediated extracellular acidification and bone resorption: Evidence for a low pH in resorbing lacunae and localization of a 100–kD lysosomal membrane protein at the osteoclast ruffled border, J. Cell Biol. 101: 2210–2222.

    Article  PubMed  CAS  Google Scholar 

  • Bekker, P. J., and Gay, C. V., 1990, Biochemical characterization of an electrogenic vacuolar proton pump in purified chicken osteoclast plasma membrane vesicles, J. Bone Miner. Res. 5: 569–579.

    Article  PubMed  CAS  Google Scholar 

  • Blair, H. C., and Schlesinger, P. H., 1990, Purification of a stilbene sensitive chloride channel and reconstitution of chloride conductivity into phospholipid vesicles, Biochem. Biophys. Res. Commun. 171: 920–925.

    Article  PubMed  CAS  Google Scholar 

  • Blair, H. C., Teitelbaum, S. L., Ghiselli, R., and Gluck, S., 1989, Osteoclastic bone resorption by a polarized vacuolar proton pump, Science 245: 855–857.

    Article  PubMed  CAS  Google Scholar 

  • Blair, H. C., Teitelbaum, S. L., Tan, H.-L., Koziol, C. M., and Schlesinger, P. H., 1991, Passive chloride permeability charge coupled to 1+-ATPase of avian osteoclast ruffled membrane, Am. J. Physiol. 260: C1315–C1324.

    PubMed  CAS  Google Scholar 

  • Blatz, A. L., and Magleby, K. L., 1983, Single voltage-dependent chloride-selective channels of large conductance in cultured rat muscle, Biophys. J. 43: 237–241.

    Article  PubMed  CAS  Google Scholar 

  • Bowman, E. J., Siegers, A., and Altendorf, K., 1988, Bafilomycins: A class of inhibitor of membrane ATPases from microorganisms, animal cells, and plant cells, Proc. Natl. Acad. Sci. USA 85: 7972–7976.

    Article  PubMed  CAS  Google Scholar 

  • Boyde, A., Ali, N. N., and Jones, S. J., 1984, Resorption of dentine by isolated osteoclasts in vitro, Br. Dent. J. 156: 216–220.

    Article  PubMed  CAS  Google Scholar 

  • Bretag, A. H., 1987, Muscle chloride channels, Physiol. Rev. 67: 618–724.

    PubMed  CAS  Google Scholar 

  • Buisman, H. P., Steinberg, T. H., Fischbarg, J., Silverstein, S. C., Vogelzang, S. A., Ince, C., Ypey, D. L., and Leijh, P. C. J., 1988, Extracellular ATP induces a large nonselective conductance in macrophage plasma membranes, Proc. Natl. Acad. Sci. USA 85: 7988–7992.

    Article  PubMed  CAS  Google Scholar 

  • Chambers, T. J., and Magnus, C. J., 1982, Calcitonin alters behaviour of isolated osteoclasts, J. Pathol. 136: 27–39.

    Article  PubMed  CAS  Google Scholar 

  • Connor, J. A., and Stevens, C. F., 1971, Voltage clamp studies of a transient outward membrane current in gastropod neural somata, J Physiol. (London) 213: 21–30.

    CAS  Google Scholar 

  • Datta, H. K., Maclntyre, I., and Zaidi, M., 1989, The effect of extracellular calcium elevation on morphology and function of isolated rat osteoclasts, Biosci. Rep. 9: 747–751.

    Article  PubMed  CAS  Google Scholar 

  • Davidson, R. M., Tatakis, D. W., and Auerbach, A. L., 1990, Multiple forms of mechanosensitive ion channels in osteoblast-like cells, Pflügers Arch. 416: 646–651.

    Article  PubMed  CAS  Google Scholar 

  • De Weer, P., Gadsby, D. C., and Rakowski, R. F., 1988, Voltage dependence of the Na–K pump, Annu. Rev. Physiol. 50: 225–241.

    Article  PubMed  Google Scholar 

  • Dos Reis, G. A., and Oliveira-Castro, G. M., 1977, Electrophysiology of phagocytic membranes. 1. Potassium-dependent slow membrane hyperpolarizations in mice macrophages, Biochim. Biophys. Acta 469: 257–263.

    Article  PubMed  Google Scholar 

  • Duncan, R., and Misler, S., 1989, Voltage-activated and stretch-activated Ba’+ conducting channels in an osteoblast-like tumor cell line (UMR 106), FEBS Lett. 251: 17–21.

    Article  PubMed  CAS  Google Scholar 

  • Ferrier, J., Ward, A., Kanehisa, J., and Heersche, J. N. M., 1986, Electrophysiological responses of osteoclasts to hormones, J. Cell. Physiol. 128: 23–26.

    Article  PubMed  CAS  Google Scholar 

  • Forgac, M., 1989, Structure and function of vacuolar class of ATP-driven proton pumps, Physiol. Rev. 69: 765–796.

    PubMed  CAS  Google Scholar 

  • Gallin, E. K., 1981, Voltage clamp studies in macrophages from mouse spleen cultures, Science 214: 458–460.

    Article  PubMed  CAS  Google Scholar 

  • Gallin, E. K., 1982, Electrophysiological properties of macrophages and macrophage-like cells, in Phagocytosis—Past and Future ( M. L. Karnovsky and L. Bolis, eds.), pp. 29–45, Academic Press, Toronto.

    Google Scholar 

  • Gallin, E. K., 1984a, Electrophysiological properties of macrophages, Fed. Proc., Fed. Am. Soc. Exp. Biol. 43: 2385–2389.

    CAS  Google Scholar 

  • Gallin, E. K., 1984b, Calcium-and voltage-activated potassium channels in human macrophages, Biophys. J. 46: 821–825.

    Article  PubMed  CAS  Google Scholar 

  • Gallin, E. K., 1986, Ionic channels in leukocytes, J. Leukocyte Biol. 39: 241–254.

    PubMed  CAS  Google Scholar 

  • Gallin, E. K., 1989, Evidence for a Ca-activated inwardly rectifying K channel in human macrophages, Am. J. Physiol. 257: C77–C85.

    PubMed  CAS  Google Scholar 

  • Gallin, E. K., 1991, Ion channels in leukocytes, Physiol. Rev. 71: 775–811.

    PubMed  CAS  Google Scholar 

  • Gallin, E. K., and Livengood, D. R., 1980, Nonlinear current–voltage relationships in cultured macrophages, J. Cell Biol. 85: 160–165.

    Article  PubMed  CAS  Google Scholar 

  • Gallin, E. K., and Livengood, D. R., 1983, Demonstration of an electrogenic Na+-K+ pump in mouse spleen macrophages, Am. J. Physiol. 245: C184–C188.

    PubMed  CAS  Google Scholar 

  • Gallin, E. K., and McKinney, L. C., 1988a, Patch-clamp studies in human macrophages: Single-channel and whole-cell characterization of two K+ conductances, J. Membr. Biol. 103: 55–66.

    Article  PubMed  CAS  Google Scholar 

  • Gallin, E. K., and McKinney, L. C., 1988b, Potassium conductances in macrophages, in Cell Physiology of Blood ( R. B. Gunn and J. C. Parker, eds.), pp. 315–332, Rockefeller University Press, New York.

    Google Scholar 

  • Gallin, E. K., and McKinney, L. C., 1990, Monovalent ion transport and membrane potential changes during activation in phagocytic leukocytes, in Current Topics in Membranes and Transport, Vol. 35 ( S. Grinstein and O. Rothstein, eds.), pp. 127–152, Academic Press, Toronto.

    Google Scholar 

  • Gallin, E. K., Wiederhold, M. L., Lipsky, P. E., and Rosenthal, A. S., 1975, Spontaneous and induced membrane hyperpolarizations in macrophages, J. Cell. Physiol. 86: 653–662.

    Article  PubMed  Google Scholar 

  • Greger, R., and Schlatter, E., 1989, Epithelial chloride channels, in Epithelial Secretion of Water and Electrolytes ( J. A. Yound and P. Y. D. Wong, eds.), Springer-Verlag, New York.

    Google Scholar 

  • Hagiwara, S., and Jaffe, L. A., 1979, Electrical properties of egg cell membranes, Annu. Rev. Biophys. Bioeng. 8: 385–416.

    Article  PubMed  CAS  Google Scholar 

  • Hall, B. K., 1991, Bone, Volume 2: The Osteoclast, CRC Press, Boca Raton, Fla.

    Google Scholar 

  • Hall, T. J., and Chambers, T. J., 1989, Optimal bone resorption by isolated rat osteoclasts requires chloride bicarbonate exchange, Calcif. Tissue Int. 45: 378–380.

    Article  PubMed  CAS  Google Scholar 

  • Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J., 1981, Improved patch-clamp techniques for high-resolution current recordings from cells and cell-free membrane patches, Pflügers Arch. 391: 85–100.

    Article  PubMed  CAS  Google Scholar 

  • Hattersley, G., and Chambers, T. J., 1989, Generation of osteoclastic function in mouse bone marrow cultures: Multinuclearity and tartrate-resistant acid phosphatase are unreliable markers for osteoclastic differentiation, Endocrinology 124: 1689–1696.

    Article  PubMed  CAS  Google Scholar 

  • Hille, B., 1984, Ionic Channels of Excitable Membranes, Sinauer, Sunderland, Mass.

    Google Scholar 

  • Ince, C., Thio, B., van Duijn, B., van Dissel, J. T., Ypey, D. L., and Leijh, P. C. J., 1987, Intracellular K+, Na+ and Cl-concentrations and membrane potential in human monocytes, Biochim. Biophys. Acta 905: 195–204.

    Article  PubMed  CAS  Google Scholar 

  • Ince, C., Coremans, J. M. C. C., Ypey, D. L., Leijh, P. C. J., Verveen, A. A., and van Furth, R., 1988, Phagocytosis by human macrophages is accompanied by changes in ionic channel currents, J. Cell Biol. 106: 1873–1878.

    Article  PubMed  CAS  Google Scholar 

  • Kakuta, Y., Okayama, H., Aikawa, T., Kanno, T., Ohyama, T., Sasaki, H., Kaito, T., and Takishima, T., 1988, K channels of human alveolar macrophages, J. Allergy Clin. Immunol. 81:460–468..

    Google Scholar 

  • Kelly, M. E. M., Arkett, S. A., Dixon, S. J., and Sims, S. M., 1991, Potassium and anion currents in rabbit osteoclasts, Biophys. J. 59: 461A (Abstract).

    Google Scholar 

  • Kelly, M. E. M., Dixon, S. J., and Sims, S. M., 1992, Inwardly rectifying potassium current in rabbit osteoclasts: A whole-cell and single-channel study, J. Membr. Biol. 126: 171–181.

    PubMed  CAS  Google Scholar 

  • Kitagawa, S., and Johnston, R. B. Jr., 1985, Relationship between membrane potential changes and superoxide-releasing capacity in resident and activated mouse peritoneal macrophages, J. Immunol. 135: 3417–3423.

    PubMed  CAS  Google Scholar 

  • Klein-Nulend, J., and Raisz, L. G., 1989, Effects of two inhibitors of anion transport on bone resorption in organ culture, Endocrinology 125: 1019–1024.

    Article  PubMed  CAS  Google Scholar 

  • Kolb, H. A., and Ubl, J., 1987, Activation of anion channels by zymosan particles in membranes of peritoneal macrophages, Biochim. Biophys. Acta 899: 239–246.

    Article  PubMed  CAS  Google Scholar 

  • Korn, S. J., Marty, A., Connor, J. A., and Horn, R., 1991, Perforated patch recording, Methods Neurosci. 4: 364–373.

    Google Scholar 

  • Malgaroli, A., Meldolesi, J., Zambonin Zallone, A., and Teti, A., 1989, Control of cytosolic free calcium in rat and chicken osteoclasts, J. Biol. Chem. 264: 14342–14347.

    PubMed  CAS  Google Scholar 

  • Marty, A., 1989, The physiological role of calcium-dependent channels, Trends Neurosci. 12: 420–424.

    Article  PubMed  CAS  Google Scholar 

  • McCann, F. V., Cole, J. J., Guyre, P. M., and Russel, J. A. G., 1983, Action potentials in macrophages derived from human monocytes, Science 219: 991–993.

    Article  PubMed  CAS  Google Scholar 

  • Mears, D. C., 1971, Effects of parathyroid hormone and thyrocalcitonin on the membrane potential of osteoclasts, Endocrinology 88: 1021–1028.

    Article  PubMed  CAS  Google Scholar 

  • Miyauchi, A., Hruska, K. A., Greenfield, E., Duncan, R., Alvarez, J., Barattolo, R., Colucci, S., ZamboninZallone, A., Teitelbaum, S. L., and Teti, A., 1990, Osteoclast cytosolic calcium, regulated by voltage-gated calcium channels and extracellular calcium, controls podosome assembly and bone resorption, J. Cell Biol. 111: 2543–2552.

    Article  PubMed  CAS  Google Scholar 

  • Nakao, M., and Gadsby, D. C., 1986, Voltage dependence of Na translocation by the Na+/K+ pump, Nature (London) 323: 628–630.

    Article  CAS  Google Scholar 

  • Nelson, D. J., Jow, B., and Jow, F., 1990a, Whole-cell currents in macrophages: I. Human monocyte-derived macrophages, J. Membr. Biol. 117: 29–44.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, D. J., Jow, B., and Popovich, K. J., 1990b, Whole-cell currents in macrophages: II. Alveolar macrophages, J. Membr. Biol. 117: 45–55.

    Article  PubMed  CAS  Google Scholar 

  • Partridge, L. D., and Swandulla, D., 1988, Calcium-activated non-specific cation channels, Trends Neurosci. 11: 69–72.

    Article  PubMed  CAS  Google Scholar 

  • Randriamampita, C., and Trautmann, A., 1987, Ionic channels in murine macrophages, J. Cell Biol. 105: 761–769.

    Article  PubMed  CAS  Google Scholar 

  • Randriamampita, C., and Trautmann, A., 1989, Biphasic increase in intracellular calcium induced by platelet-activating factor in macrophages, FEBS Lett. 249: 199–206.

    Article  PubMed  CAS  Google Scholar 

  • Ravesloot, J. H., 1991, Ion channels in bone cells, Ph.D. thesis, The University of Leiden, Leiden, The Netherlands.

    Google Scholar 

  • Ravesloot, J. H., Ypey, D. L., Nijweide, P. J., Buisman, H. P., Weidema, F., and Vrijheid-Lammers, T., 1989a, Three voltage activated K+ conductances and a by extracellular ATP activated conductance in freshly isolated embryonic chick osteoclasts, J. Bone Miner. Res. 4 (Suppl. 1): S200 (Abstract).

    Google Scholar 

  • Ravesloot, J. H., Ypey, D. L., Vrijheid-Lammers, T., and Nijweide, P. J., 1989b, Voltage-activated K+ conductances in freshly isolated embryonic chicken osteoclasts, Proc. Natl. Acad. Sci. USA 86: 6821–6825.

    Article  PubMed  CAS  Google Scholar 

  • Riches, D. W. H., Channon, J. Y., Leslie, C. C., and Henson, P. M., 1988, Receptor-mediated signal transduction in mononuclear phagocytes, Prog. Allergy 42: 65–112.

    PubMed  CAS  Google Scholar 

  • Rudy, B., 1988, Diversity and ubiquity of K channels, Neuroscience 25: 729–749.

    Article  PubMed  CAS  Google Scholar 

  • Sakmann, B., and Trube, G., 1984, Conductance properties of single inwardly rectifying potassium channels in ventricular cells from guinea-pig heart, J. Physiol. (London) 347: 641–657.

    CAS  Google Scholar 

  • Schoppa, N. A., Su, Y., Baron, R., and Boulpaep, E. L., 1990, Identification of single ion channels in neonatal rat osteoclasts, J. Bone Mineral Res. 5 (Suppl. 2): S204 (Abstract).

    Google Scholar 

  • Schwarze, W., and Kolb, H. A., 1984, Voltage dependent kinetics of an anionic channel of large unit conductance in macrophages and myotube membranes, Pflügers Arch. 402: 281–291.

    Article  PubMed  CAS  Google Scholar 

  • Sims, S. M., and Dixon, S. J., 1989, Inwardly rectifying K+ current in osteoclasts, Am. J. Physiol. 256: C1277–C1282.

    PubMed  CAS  Google Scholar 

  • Sims, S. M., Kelly, M. E. M., and Dixon, S. J., 1991, K’ and Cl-currents in freshly isolated rat osteoclasts, Pflügers Arch. 419: 358–370.

    Article  PubMed  CAS  Google Scholar 

  • Sims, S. M., Kelly, M. E. M., Arkett, S. A., and Dixon, S. J., 1992, Electrophysiology of osteoclasts, in Biology and Physiology of the Osteoclast ( B. R. Rifkin and C. V. Gay, eds.), CRC Press, Boca Raton, Fla. pp. 223–244.

    Google Scholar 

  • Standen, N. B., and Stanfield, P. R., 1978, A potential-and time-dependent blockade of inward rectification in frog skeletal muscle fibres by barium and strontium ions, J. Physiol. (London) 280: 169–191.

    CAS  Google Scholar 

  • Sundquist, K., Lakkakorpi, P., Wallmark, B., and Väänänen, K., 1990, Inhibition of osteoclast proton transport by bafilomycin A, abolishes bone resorption, Biochem. Biophys. Res. Commun. 168: 309–313.

    Article  PubMed  CAS  Google Scholar 

  • Teti, A., Blair, H. C., Teitelbaum, S. L., Kahn, A. J., Koziol, C., Konsek, J., Zambonin-Zallone, A., and Schlesinger, P. H., 1989, Cytoplasmic pH regulation and chloride/bicarbonate exchange in avian osteoclasts, J. Clin. Invest. 83: 227–233.

    Article  PubMed  CAS  Google Scholar 

  • Väänänen, H. K., Karhukorpi, E.-K., Sundquist, K., Wallmark, B., Roininen, I., Hentunen, T., Tuukkanen, J., and Lakkakorpi, P., 1990, Evidence for the presence of a proton pump of the vacuolar H+-ATPase type in the ruffled borders of osteoclasts, J. Cell Biol. 111: 1305–1311.

    Article  PubMed  Google Scholar 

  • Vaes, G., 1988, Cellular biology and biochemical mechanism of bone resorption. A review of recent developments on the formation, activation, and mode of action of osteoclasts, Clin. Orthop. Rel. Res. 231: 239–271.

    CAS  Google Scholar 

  • Wood, D. A., Hapak, L. K., Sims, S. M., and Dixon, S. J., 1991, Direct effects of platelet-activating factor on isolated rat osteoclasts: Rapid elevation of intracellular free calcium and transient retraction of pseudopode, J. Biol. Chem. 266: 15369–15376.

    PubMed  CAS  Google Scholar 

  • Young, J. D.-E., Unkeless, J. C., Kaback, H. R., and Cohn, Z. A., 1983a, Mouse macrophage Fc receptor for IgG gamma 2b/gamma 1 in artificial and plasma membrane vesicle functions as a ligand-dependent ionophore, Proc. Natl. Acad. Sci. USA 80: 1636–1640.

    Article  PubMed  CAS  Google Scholar 

  • Young, J. D.-E., Unkeless, J. C., Young, T. M., Mauro, A., and Cohn, Z. A., 1983b, Role for mouse macrophage IgG Fc receptor as ligand-dependent ion channel, Nature (London) 306: 186–189.

    CAS  Google Scholar 

  • Ypey, D. L., and Clapham, D. E., 1984, Development of a delayed outward-rectifying K+ conductance in cultured mouse peritoneal macrophages, Proc. Natl. Acad. Sci. USA 81: 3083–3087.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Oral Biology and Department of Physiology, Faculty of Dentistry, The University of Western Ontario, London, Ontario, Canada, N6A 5C1

    S. Jeffrey Dixon

  2. Department of Physiology, Faculty of Dentistry, The University of Western Ontario, London, Ontario, Canada, N6A 5C1

    Stuart A. Arkett & Stephen M. Sims

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  1. S. Jeffrey Dixon
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  1. Imperial Cancer Research Fund Haemopoiesis Research Group, Department of Haematology, St. Bartholomew’s Hospital Medical College, EC1A 7BE, London, UK

    Michael A. Horton

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Dixon, S.J., Arkett, S.A., Sims, S.M. (1993). Electrophysiology of Osteoclasts and Macrophages. In: Horton, M.A. (eds) Macrophages and Related Cells. Blood Cell Biochemistry, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9534-9_8

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