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Antigens of Osteoclasts Phenotypic Definition of a Specialized Hemopoietic Cell Lineage

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Part of the Blood Cell Biochemistry book series (BLBI,volume 5)

Abstract

Immunological approaches have readily found a place in the analysis of hemopoietic differentiation and led to the definition of cellular subpopulations which previously had only been identified by applying functional criteria. Increasingly, the techniques of monoclonal antibody technology, biochemical analysis, and, more recently, molecular cloning have been applied to questions of bone cell biology. In this chapter we review the current literature on the expression of antibody-defined antigens of osteoclasts, bone-resorbing cells of hemopoietic origin. The definition of the immunophenotype of human osteoclasts is considered primarily, but, where relevant, features of avian and rodent cells are also discussed. Our knowledge of the function of the majority of osteoclast antigens is limited; we do, however, speculate about a role in bone for some molecules, for example, aminopeptidase N (CD13), CD9, ICAM-1 (CD54), and the transferrin receptor (CD71). A section on adhesive molecules, mainly integrins, is included as they were among the first “specific” markers of human osteoclasts to be reported (the vitronectin receptor, CD51, by Horton et al., 1985a) and the definition of their functional activity in osteoclasts is well advanced. Finally, we review the application of immunological techniques to the phenotypic analysis of cells of the mononuclear phagocyte system and compare their characteristics with those of osteoclasts, particularly as there has been considerable dispute over their exact interrelationship.

Keywords

  • Bone Resorption
  • Giant Cell
  • Giant Cell Tumor
  • Mononuclear Phagocyte System
  • Hemopoietic Cell

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Alvarez, J. I., Teitelbaum, S. L., Chappel, J. C., Cheresh, D. A., Sander, D., Farach-Carson, M. C., Robey, P. G., and Ross, F. P., 1990, Osteoclast attachment to bone and its subsequent resorption depend upon the vitronectin receptor, J. Bone Miner. Res. 5: S154 (Abstract No. 321).

    Google Scholar 

  • Andreesen, R., Brugger, W., Scheibenbogen, C., Kreutz, M., Leser, H. G., Rehm, A., and Lohr, G. W., 1990, Surface phenotype analysis of human monocyte to macrophage maturation, J. Leukocyte Biol. 47: 490–497.

    PubMed  CAS  Google Scholar 

  • Agel, N. M., Clark, M., Jones, D. B., Arnot, J., and Waldmann, H., 1987, Identification of cells of the human mononuclear phagocyte system with rat monoclonal antibodies, J. Pathol. 152: 265–273.

    CrossRef  Google Scholar 

  • Agel, N. M., Pringle, J. A. S., and Horton, M. A., 1988, Cellular heterogeneity in giant cell tumour of bone (osteoclastoma): An immunohistological study of 16 cases, Histopathology 13: 675–685.

    CrossRef  Google Scholar 

  • Ashmun, R. A., and Look, A. T., 1990, Metalloprotease activity of CD 13/aminopeptidase N on the surface of human myeloid cells, Blood 75: 462–469.

    CAS  Google Scholar 

  • Athanasou, N. A., and Quinn, J., 1990, Immunophenotypic differences between osteoclasts and macrophage polykaryons: Immunohistological distinction and implications for osteoclast ontogeny and function, J. Clin. Pathol. 43: 997–1003.

    PubMed  CrossRef  CAS  Google Scholar 

  • Athanasou, N. A., Quinn, J., and McGee, J. 0.-D., 1988a, Immunocytochemical analysis of the human osteoclast: Phenotypic relationship to other marrow-derived cells, Bone Miner. 3: 317–333.

    CAS  Google Scholar 

  • Athanasou, N. A., Pulford, K. A. F., Quinn, J., Gatter, K. C., Mason, D. Y., and McGee, J. 0., 1988b, A monoclonal antimacrophage antibody (3MA-134) which reacts with human osteoclasts in decalcified paraffin embedded bone sections, Calcif. Tissue Int. 42 (Suppl.): A1.

    Google Scholar 

  • Athanasou, N. A., Quinn, J., and McGee, J. O’D., 1989a, Reactivity of Workshop mAb of the myeloid, platelet, and non-lineage (CD45, 45R, CD 11 a, b, c) series with cells in bone and synovium, in Leucocyte Typing IV. White Cell Differentiation Antigens ( W. Knapp, B. Dörken, W. R. Gilks, E. P. Rieber, R. E. Schmidt, H. Stein, and A. E. G. Kr. von dem Borne, eds.), pp. 921–923, Oxford University Press, Oxford.

    Google Scholar 

  • Athanasou, N. A., Wells, C. A., Quinn, J., Ferguson, D. P., Heryet, A., and McGee, J. O., 1989b, The origin and nature of stromal osteoclast-like multinucleated giant cells in breast carcinoma: Implications of tumour osteolysis and macrophage biology, Br. J. Cancer 59: 491–498.

    PubMed  CrossRef  CAS  Google Scholar 

  • Athanasou, N. A., Alvarez, J. I., Ross, F. P., Quinn, J., and Teitelbaum, S. L., 1992, Species differences in the immunophenotype of osteoclasts and mononuclear phagocytes, Calcif. Tissue Int. 50: 427–432.

    PubMed  CrossRef  CAS  Google Scholar 

  • Athanasou, N. A., Puddle, B., Quinn, J., and Woods, C. G., 1991, Use of monoclonal antibodies to recognise osteoclasts in routinely processed bone biopsy specimens, J. Clin. Pathol. 44: 664–666.

    PubMed  CrossRef  CAS  Google Scholar 

  • Backe, E., Schwarting, R., Gerdes, J., Ernst, M., and Stein, H., 1991, BerMac3: New monoclonal antibody that defines human monocyte/macrophage differentiation antigen, J. Clin. Pathol. 44: 936–945.

    PubMed  CrossRef  CAS  Google Scholar 

  • Bossy, B., and Reichardt, L. F., 1990, Chicken integrin a subunit molecular analysis reveals high conservation of structural domains and association with multiple ß subunits in embryo fibroblasts, Biochemistry 29: 10191–10198.

    PubMed  CrossRef  CAS  Google Scholar 

  • Chambers, T. J., and Horton, M. A., 1984, Failure of cells of the mononuclear phagocyte series to resorb bone, Calcif. Tissue Int. 36: 556–558.

    PubMed  CrossRef  CAS  Google Scholar 

  • Chambers, T. J., Fuller, K., Darby, J. A., Pringle, J. A. S., and Horton, M. A., 1986, Monoclonal antibodies against osteoclasts inhibit bone resorption in vitro, Bone Miner. 1: 127–135.

    PubMed  CAS  Google Scholar 

  • Chilosi, M., Giliol, E., Lestani, M., Menestrina, F., and Fiore-Donati, L., 1988, Immunohistochemical characterization of osteoclasts and osteoclast-like cells with monoclonal antibody MB1 on paraffin-embedded tissues, J. Pathol. 156: 251–254.

    PubMed  CrossRef  CAS  Google Scholar 

  • Clover, J., Dodds, R. A., and Gowen, M., 1992, Integrin subunit expression by human osteoblasts and osteoclasts in situ and in culture, J. Cell Sci. 103: 267–271.

    PubMed  CAS  Google Scholar 

  • Coombe, D. R., and Rider, C. C., 1989, Lymphocyte homing receptors cloned—a role for anionic polysaccharides in lymphocyte adhesion, Immunol. Today 10: 289–291.

    PubMed  CrossRef  CAS  Google Scholar 

  • Cottrell, B., and Jones, D., 1990, Functional and phenotypic changes associated with the in vitro development of human monocytes into macrophages, FEMS Microbiol. Immunol. 2: 333–337.

    PubMed  CrossRef  CAS  Google Scholar 

  • Davies, J. D., 1990, The use of monoclonal antibodies to identify and characterise functional antigens on osteoclasts, Ph.D. Thesis, University of London.

    Google Scholar 

  • Davies, J., Warwick, J., Totty, N., Philp, R., Helfrich, M., and Horton, M., 1989, The osteoclast functional antigen implicated in the regulation of bone resorption is biochemically related to the vitronectin receptor, J. Cell Biol. 109: 1817–1826.

    PubMed  CrossRef  CAS  Google Scholar 

  • D’Souza, S. E., Ginsberg, M. H., Burke, T. A., and Plow, E. F., 1990, The ligand binding site of the platelet integrin receptor GPIIb-IIIa is proximal to the second calcium binding domain of its alpha subunit, J. Biol. Chem. 265: 3440–3446.

    PubMed  Google Scholar 

  • Duijvestijn, A., and Hamann, A., 1989, Mechanisms and regulation of lymphocyte migration, Immunol. Today 10: 23–28.

    PubMed  CrossRef  CAS  Google Scholar 

  • Flanagan, A. M., and Chambers, T. J., 1989, Osteoclasts are present in the giant cell variant of malignant fibrous histiocytoma, J. Pathol. 159: 53–57.

    PubMed  CrossRef  CAS  Google Scholar 

  • Flanagan, A. M., Horton, M. A., Dorey, E. L., Evely, R. S., Moseley, J. M., Firkin, F. C., Chambers, T. J., Helfrich, M. H., and Martin, T. J., 1992, An assessment of the ability of human bone marrow cultures to generate osteoclasts, Int. J. Pathol. 73: 387–401.

    CAS  Google Scholar 

  • Fuller, K., and Chambers, T. J., 1987, Generation of osteoclasts in cultures of rabbit bone marrow and spleen cells, J. Cell. Physiol. 132: 441–452.

    PubMed  CrossRef  CAS  Google Scholar 

  • Goldberg, R. D., Michelassi, F., and Montag, A. G., 1991, Osteoclast-like giant cell tumor of the pancreas: Immunophenotypic similarity to giant cell tumor of bone, Hum. Pathol. 22: 618–622.

    PubMed  CrossRef  CAS  Google Scholar 

  • Gordon, S., Keshav, S., and Chung, L. P., 1988, Mononuclear phagocytes: Tissue distribution and functional heterogeneity, Curr. Opin. Immunol. 1: 26–35.

    PubMed  CrossRef  CAS  Google Scholar 

  • Greywoode, G. I. N., McCarthy, S. P., and McGee, J. O’D., 1990, Labelling of cells of the mononuclear phagocyte system in routinely processed archival biopsy specimens with monoclonal antibody EBM/ 11, J. Clin. Pathol. 43: 992–996.

    CAS  Google Scholar 

  • Hato, T., Ikeda, K., Yasukawa, M., Watanabe, A., and Kobayashi, Y., 1988, Exposure of platelet fibrinogen receptors by a monoclonal antibody to CD9 antigen, Blood 72: 224–229.

    PubMed  CAS  Google Scholar 

  • Hattersley, G., and Chambers, T. J., 1989, Calcitonin receptors as markers for osteoclastic differentiation: Correlation between generation of bone resorptive cells and cells that express calcitonin receptors in mouse bone marrow cultures, Endocrinology 125: 1606–1612.

    PubMed  CrossRef  CAS  Google Scholar 

  • Hattersley, G., Kerby, J. A., and Chambers, T. J., 1991, Identification of osteoclast precursors in multilineage colonies, Endocrinology 128: 259–262.

    PubMed  CrossRef  CAS  Google Scholar 

  • Helfrich, M. H., and Horton, M. A., 1992, Adhesion to bone matrix protein by chick osteoclasts differs from that by rat osteoclasts, in Bone Biology and Skeletal Disorders, ( C. C. Whitehead, ed.), p. 361, Carfax Publishing Co., United Kingdom.

    Google Scholar 

  • Helfrich, M. H., Nesbitt, S. A., and Horton, M. A., 1992a, Integrins on rat osteoclasts: Characterisation of two monoclonal antibodies (F4 and F11) to rat $3, J. Bone Miner. Res. 7: 345–351.

    PubMed  CrossRef  CAS  Google Scholar 

  • Helfrich, M. H., Nesbitt, S. A., Dorey, E. L., and Horton, M. A., 1992b, Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectin via a $3 integrin, J. Bone Miner. Res. 7: 335–343.

    PubMed  CrossRef  CAS  Google Scholar 

  • Hemler, M. E., 1990, VLA proteins in the integrin family: Structures, functions, and their role on leukocytes, Annu. Rev. Immunol. 82: 365–400.

    CrossRef  Google Scholar 

  • Hentunen, T. A., and Väänänen, H. K., 1989, Inhibition of bone resorption by osteoclast-recognizing monoclonal antibodies, J. Bone Miner. Res. 4 (Suppl. 1): S213.

    Google Scholar 

  • Hentunen, T. A., Tuukkanen, J., and Väänänen, H. K., 1990, Osteoclasts and a small population of peripheral blood cells share common surface antigens, Calcif. Tissue Int. 47: 8–17.

    PubMed  CrossRef  CAS  Google Scholar 

  • Hentunen, T. A., Lakkakorpi, P. T., Rautiala, T., and Väänänen, K., 1991, Inhibition of bone resorption by a monoclonal antibody that reacts with a 150 kD membrane protein in chicken osteoclasts, J. Bone Miner. Res. 6: 1091–1097.

    PubMed  CrossRef  CAS  Google Scholar 

  • Horejsi, V., 1991, A novel family of leukocyte surface antigens, Immunol. Today 12: 287.

    PubMed  CrossRef  CAS  Google Scholar 

  • Horton, M., 1990, Current status review. Vitronectin receptor: Tissue specific expression or adaptation to culture, Int. J. Exp. Pathol. 71: 741–759.

    PubMed  CAS  Google Scholar 

  • Horton, M. A., 1988, Osteoclast-specific antigens, LS/ Atlas Sci. Immunol. 1: 35–43.

    Google Scholar 

  • Horton, M. A., and Chambers, T. J., 1986, Human osteoclast specific antigens are expressed by osteoclasts in a wide range of non-human species, Br. J. Exp. Pathol. 67: 95–104.

    PubMed  CAS  Google Scholar 

  • Horton, M. A., and Davies, J., 1989, Perspectives: Adhesion receptors in bone, J. Bone Miner. Res. 4: 803–808.

    CAS  Google Scholar 

  • Horton, M. A., and Helfrich, M. H., 1992, Antigenic markers of osteoclasts, in Biology and Physiology of the Osteoclast ( B. R. Rifkin and C. Gay, eds.), pp. 33–54, CRC Press, Boca Raton, Fla.

    Google Scholar 

  • Horton, M. A., Rimmer, E. F., Lewis, D., Pringle, J. A. S., Fuller, K., and Chambers, T. J., 1984, Cell surface characterisation of the human osteoclast: Phenotypic relationship to other bone marrow cell types, J. Pathol. 144: 281–294.

    PubMed  CrossRef  CAS  Google Scholar 

  • Horton, M. A., Lewis, D., McNulty, K., Pringle, J. A. S., and Chambers, T. J., 1985a, Monoclonal antibodies to osteoclastomas (giant cell bone tumours): Definition of osteoclast-specific antigens, Cancer Res. 45: 5663–5669.

    PubMed  CAS  Google Scholar 

  • Horton, M. A., Rimmer, E. F., Moore, A., and Chambers, T. J., 1985b, On the origin of the osteoclast: The cell surface phenotype of rodent osteoclasts, Calcif. Tissue Int. 37: 46–50.

    PubMed  CrossRef  CAS  Google Scholar 

  • Horton, M. A., Dorey, E., Helfrich, M. H., and Simpson, A. F. P., 1989, Cluster analysis of platelet and myeloid antibody panels: Contribution of immunohistological analysis of osteoclasts and other tissues, in Leucocyte Typing IV. White Cell Differentiation Antigens ( W. Knapp, B. Dörken, W. R. Gilks, E. P. Reiber, R. E. Schmidt, H. Stein, and A. E. G. Kr. von dem Borne, eds.), pp. 979–981, Oxford University Press, Oxford.

    Google Scholar 

  • Horton, M. A., Taylor, M. L., Arnett, T. R., and Helfrich, M. H., 1991, Arg-Gly-Asp (RGD) peptides and the antivitronectin receptor antibody 23C6 inhibit cell spreading and dentine resorption by osteoclasts, Exp. Cell Res. 195: 368–375.

    PubMed  CrossRef  CAS  Google Scholar 

  • Horton, M. A., Dorey, E. L., Nesbitt, S., Samanen, J., Ali, F. E., Stadel, J. M., Nichols, A., Greig, R., and Helfrich, M. H., 1993, Modulation of vitronectin receptor-mediated osteoclast adhesion by Arg-GlyAsp peptide analogs: a structure-function analysis, J Bone Min. Res. 8: 241–249.

    Google Scholar 

  • Hume, D. A., Loutit, J. F., and Gordon, S., 1984, The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80: Macrophages of bone and associated connective tissue, J. Cell Sci. 66: 189–194.

    PubMed  CAS  Google Scholar 

  • Hunkapiller, T., and Hood, L., 1989, Diversity of the immunoglobulin gene superfamily, Adv. Immunol. 44: 1–63.

    PubMed  CrossRef  CAS  Google Scholar 

  • Hynes, R., 1987, Integrins: A family of cell surface receptors, Cell 48: 549–554.

    PubMed  CrossRef  CAS  Google Scholar 

  • Hynes, R. 0., 1992, Integrins: versatility, modulation and signaling in cell adhesion, Cell 69: 11–25.

    CAS  Google Scholar 

  • Indig, F. E., Benayallu, D., Fried, A., Wientroub, S., and Blumberg, S., 1990, Neutral endopeptidase (EC 3.4.24.11) is highly expressed on osteoblastic cells and other marrow stromal cell types, Biochem.

    Google Scholar 

  • Biophys. Res. Commun. 172:620–626.

    Google Scholar 

  • James, I. E., Walsh, S., Dodds, R. A., and Gowen, M., 1991, Production and characterization of osteoclastselective monoclonal antibodies which distinguish between multinucleated cells derived from different human tissues, J. Histochem. Cytochem. 39: 905–914.

    PubMed  CrossRef  CAS  Google Scholar 

  • Ketcham, C. M., Roberts, R. M., Simmen, R. C. M., and Nick, H. S., 1989, Molecular cloning of the type 5, iron-containing, tartrate-resistant acid phosphatase from human placenta, J. Biol. Chem. 264: 557–563.

    PubMed  CAS  Google Scholar 

  • Knapp, W., Dörken, B., Gilks, W. R., Reiber, E. P., Schmidt, R. E., Stein, H., and von dem Borne, A. E. G. Kr. (eds.), 1989, Leucocyte Typing IV. White Cell Differentiation Antigens, Oxford University Press, Oxford.

    Google Scholar 

  • Kukita, T., and Roodman, G. D., 1989, Development of a monoclonal antibody to osteoclasts formed in vitro which recognizes mononuclear osteoclast precursors in the marrow, Endocrinology 125: 630–637.

    PubMed  CrossRef  CAS  Google Scholar 

  • Kukita, T., McManus, L. M., Miller, M., Civin, C., and Roodman, G. D., 1989, Osteoclast-like cells formed in long-term human bone marrow cultures express a similar surface phenotype as authentic osteoclasts, Lab. Invest. 60: 532–538.

    PubMed  CAS  Google Scholar 

  • Kurihara, N., Bertolini, D., Suda, T., Akayama, Y., and Roodman, G. D., 1990, IL-6 stimulated osteoclast-like multinucleated cell formation in long-term human cultures by inducing IL-1 release, J. Immunol. 144: 4226–4230.

    PubMed  CAS  Google Scholar 

  • Lakkakorpi, P. T., Horton, M. A., Helfrich, M. H., Karhukorpi, E.-K., and Väänänen, H. K., 1991, Vitronectin receptor has a role in bone resorption but does not mediate tight sealing zone attachment of osteoclasts to the bone surface, J. Cell Biol. 115: 1179–1186.

    PubMed  CrossRef  CAS  Google Scholar 

  • Lanza, F., Wolf, D., Fox, C. F., Kieffer, N., Seyer, J. M., Fried, V. A., Coughlin, S. R., Phillips, D. R., and Jennings, L. K., 1991, cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane spanning proteins, J. Biol. Chem. 266: 10638–10645.

    Google Scholar 

  • Lasky, L. A., 1991, Lectin cell adhesion molecules (LEC-CAMS): A new family of cell adhesion proteins involved with inflammation, J. Cell. Biochem. 45: 139–146.

    PubMed  CrossRef  CAS  Google Scholar 

  • Leenen, P. J. M., Melis, M., Kraal, G., Hoogeveen, A. T., and van Ewijk, W., 1989, Monoclonal antibody ER-BMDM 1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity, in Phenotypical analysis of murine macrophage differentiation (P. J. M. Leenen, Ph.D. Thesis ), University of Rotterdam.

    Google Scholar 

  • MacDonald, B. R., Mundy, G. R., Clark, S., Wang, E. A., Kuehl, T. J., Stanley, E. R., and Roodman, G. D., 1986, Effects of human recombinant CSF-GM and high purified CSF-1 on the formation of multinucleated cells with osteoclast characteristics in longterm bone marrow cultures, J. Bone Miner. Res. 1: 227–233.

    PubMed  CrossRef  CAS  Google Scholar 

  • MacDonald, B. R., Takahashi, N., McManus, L. M., Holohan, J., Mundy, G. R., and Roodman, G. D., 1987, Formation of multinucleated cells that respond to osteotropic hormones in long term human marrow cultures, Endocrinology 120: 2326–2333.

    PubMed  CrossRef  CAS  Google Scholar 

  • Marcantonio, E. E., and Hynes, R. 0., 1988, Antibodies to the conserved cytoplasmic domain of the integrin ß subunit react with proteins in vertebrates, invertebrates, and fungi, J. Cell Biol. 106: 1765–1772.

    CAS  Google Scholar 

  • Maxfield, S. R., Moulder, K., Koning, F., Elbe, S., Stingl, G., Coligan, J. E., Shevach, E. M., and Yokovama, W. M., 1989, Murine T cells express a cell surface receptor for multiple extracellular matrix protein. Identification and characterization with monoclonal antibodies, J. Exp. Med. 169: 2173–2190.

    PubMed  CrossRef  CAS  Google Scholar 

  • McEver, R. P., 1991, GMP-140: A receptor for neutrophils and monocytes on activated platelets, J. Cell. Biochem. 45: 156–161.

    PubMed  CrossRef  CAS  Google Scholar 

  • McMichael, A. J., Beverley, P. L. C., Cobbold, S., Crumpton, M. J., Gilks, W., Gotch, F. M., Hogg, N., Horton, M. A., Ling, N., MacLennan, I. C. M., Mason, D. Y., Milstein, C., Spiegelhalter, D., and Waldmann, H. (eds.), 1987, Leucocyte Typing III. White Cell Differentiation Antigens, Oxford University Press, Oxford.

    Google Scholar 

  • Miyauchi, A., Alvarez, J., Greenfield, E. M., Teti, A., Grano, M., Colucci, S., Zambonin-Zallone, A., Ross, F. P., Teitebaum, S. L., Cheresh, D., and Hruska, K. A., 1991, Recognition of osteopontin and related peptides by an avß3 integrin stimulates immediate cell signals in osteoclasts, J. Biol. Chem. 266: 20369–20374.

    PubMed  CAS  Google Scholar 

  • Most, J., Neumayer, H. P., and Dierich, M. P., 1990, Cytokine-induced generation of multinucleated giant cells in vitro requires interferon-gamma and expression on LFA-1, Eur. J. Immunol. 20: 1661–1667.

    PubMed  CrossRef  CAS  Google Scholar 

  • Moulder, K., Roberts, K., Shevach, E. M., and Coligan, J. E., 1991, The mouse vitronectin receptor is a T cell activation antigen, J. Exp. Med. 173: 343–347.

    PubMed  CrossRef  CAS  Google Scholar 

  • Nelson, P. N., Pringle, J. A. S., and Chambers, T. J., 1991, Production and characterisation of new monoclonal antibodies to human osteoclasts, Calcif. Tissue Int. 49: 317–320.

    PubMed  CrossRef  CAS  Google Scholar 

  • Nesbitt, S., Hart, I., and Horton, M. A., 1989, Epitope analysis of the vitronectin receptor (CD51), in Leucocyte Typing IV. White Cell Differentiation Antigens ( W. Knapp, B. Dörken, W. R. Gilks, E. P. Reiber, R. E. Schmidt, H. Stein, and A. E. G. Kr. von dem Borne, eds.), pp. 1037, Oxford University Press, Oxford.

    Google Scholar 

  • Nesbitt, S., Nesbit, A., Helfrich, M., and Horton, M., 1993, Biochemical characterisation of human osteoclast integrins: osteoclasts express a v 03,a20, and a„ß,integrins, J. Biol. Chem.,in press.

    Google Scholar 

  • Nijweide, P. J., Vrijheid-Lammers, T., Mulder, R. J., and Blok, J., 1985, Cell surface antigens on osteoclasts and related cells in the quail studied with monoclonal antibodies, Histochemistry 83:315–324. Osborn, L., 1990, Leukocyte adhesion to endothelium in inflammation, Cell 62: 3–6.

    Google Scholar 

  • Oursler, M. J., and Osdoby, P., 1988, Osteoclast development in marrow cultured in calvaria-conditioned media, Dev. Biol. 127: 170–178.

    PubMed  CrossRef  CAS  Google Scholar 

  • Oursler, M. J., Bell, L. V., Clevinger, B., and Osdoby, P., 1985, Identification of osteoclast-specific monoclonal antibodies, J. Cell Biol. 100: 1592–1600.

    PubMed  CrossRef  CAS  Google Scholar 

  • Oursler, M. J., Li, L., and Osdoby, P., 1989, Characterization of an osteoclast membrane protein related to superoxide dismutase, J. Bone Miner. Res. 4 (Suppl. 1): S265.

    Google Scholar 

  • Parravicini, C. L., Soligo, D., Berti, E., Cattoretti, G., Gaiera, G., and Vago, L., 1989, Immunohistochemical reactivity of anti-platelet mAb in normal human tissues and bone marrow, in Leucocyte Typing IV. White Cell Differentiation Antigens ( W. Knapp, B. Dörken, W. R. Gilks, E. P. Reiber, R. E. Schmidt, H. Stein, and A. E. G. Kr. von dem Borne, eds.), pp. 981–985, Oxford University Press, Oxford.

    Google Scholar 

  • Quinn, J., Athanasou, N. A., and McGee, J. O’D., 1991, Extracellular matrix receptor and platelet antigens on osyteoclasts and foreign body giant cells, Histochemistry 96: 169–176.

    CAS  Google Scholar 

  • Radzun, H. J., Kreipe, H., Bodewadt, S., Hausmann, M.-L., Barth, J., and Parwaresch, M. R., 1987, KiM8 monoclonal antibody reactive with an intracytoplasmic antigen of monocyte/macrophage lineage, Blood 69: 1320–1327.

    PubMed  CAS  Google Scholar 

  • Reinholt, F. P., Hultenby, K., Oldberg, A., and Heinegard, D., 1990, Osteopontin—a possible anchor of osteoclasts to bone, Proc. Natl. Acad. Sci. USA 87: 4473–4475.

    PubMed  CrossRef  CAS  Google Scholar 

  • Rifkin, B. R., and Gay, C. (eds.), 1992, Biology and Physiology of the Osteoclast,CRC Press, Boca Raton, Fla., in press.

    Google Scholar 

  • Ruitishauser, U., and Jessell, T. M., 1988, Cell adhesion molecules in vertebrate neural development, Physiol. Rev. 68: 819–857.

    Google Scholar 

  • Sato, M., Sardana, M. K., Grasser, W. A., Garsky, V. M., Murray, J. M., and Gould, R. J., 1990, Echistatin is a potent inhibitor of bone resorption in culture, J. Cell Biol. 111: 1713–1723.

    PubMed  CrossRef  CAS  Google Scholar 

  • Schneider, G. B., and Relfson, M., 1988, The effects of transplantation of granulocyte–macrophage progenitors on bone resorption in osteopetrotic rats, J. Bone Miner. Res. 3: 225–232.

    PubMed  CrossRef  CAS  Google Scholar 

  • Slupsky, J. R., Seehafer, J. G., Tang, S.-S., Masellis Smith, A., and Shaw, A. R. E., 1989, Evidence that monoclonal antibodies against CD9 antigen induce specific association between CD9 and the platelet glycoprotein IIb–IIIa complex, J. Biol. Chem. 264: 12289–12293.

    PubMed  CAS  Google Scholar 

  • Sminia, T., and Dijkstra, C. D., 1986, The origin of osteoclasts: An immunohistochemical study on macrophages and osteoclasts in embryonic rat bone, Calcif. Tissue Int. 39: 263–266.

    PubMed  CrossRef  CAS  Google Scholar 

  • Springer, T. A., and Lasky, L. A., 1991, Sticky sugars for selectins, Nature (London) 349:196–197. Stoolman, L. M., 1989, Adhesion molecules controlling lymphocyte migration, Cell 56: 907–910.

    Google Scholar 

  • Takahashi, N., Akatsu, T., Udagawa, N., Sasaki, T., Yamaguchi, A., Moseley, J. M., Martin, T. J., and Suda, T., 1988, Osteoblastic cells are involved in osteoclast formation, Endocrinology 123: 2600–2602.

    PubMed  CrossRef  CAS  Google Scholar 

  • Takeichi, M., 1988, The cadherins: Cell—cell adhesion molecules controlling animal morphogenesis, Development 102: 639–655.

    PubMed  CAS  Google Scholar 

  • Takeya, M., Tsuchiya, T., Shimokawa, Y., and Takahashi, K., 1991, A new monoclonal antibody, PM-2K, specifically recognises tissue macrophages but not blood monocytes, J. Pathol. 163: 315–321.

    PubMed  CrossRef  CAS  Google Scholar 

  • Werb, Z., Tremble, P. M., Behrendtsen, O., Crowley, E., and Damsky, C. H., 1989, Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression, J. Cell Biol. 109: 877–889.

    PubMed  CrossRef  CAS  Google Scholar 

  • Zambonin-Zallone, A., Teti, A., Grano, M., Rubinacci, A., Abbadini, M., Gaboli, M., and Marchisio, P. C., 1989, Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: A $3 antigen is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells, Exp. Cell Res. 182: 645–652.

    PubMed  CrossRef  CAS  Google Scholar 

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Helfrich, M.H., Horton, M.A. (1993). Antigens of Osteoclasts Phenotypic Definition of a Specialized Hemopoietic Cell Lineage. In: Horton, M.A. (eds) Macrophages and Related Cells. Blood Cell Biochemistry, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9534-9_7

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