Abstract
Minsky’s original confocal microscope (1957) scans a specimen under a spot of light and detects emission from the same spot, using circular apertures to define both the illuminating and the emitted beams. Many subsequent designs have used multiple or slit-shaped apertures, retaining some of the confocal effect but permitting faster imaging. In some of these microscopes, the succession of optical images is so fast that the flicker-fusion frequency (ca. 18 Hz) of human vision is exceeded, giving an impression of continuous, direct viewing. This chapter concentrates on the type of direct view confocal microscope that uses a slit aperture and an imaging detector. Various forms of this microscope are now available commercially and their use seems likely to increase.
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Amos, W.B., White, J.G. (1995). Direct View Confocal Imaging Systems Using a Slit Aperture. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_25
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DOI: https://doi.org/10.1007/978-1-4757-5348-6_25
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