Handbook of Biological Confocal Microscopy

  • James B. Pawley

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. David R. Sandison, Rebecca M. Williams, K. Sam Wells, James Strickler, Watt W. Webb
    Pages 39-53
  3. Robert H. Webb, C. Kathleen Dorey
    Pages 55-67
  4. Enrico Gratton, Martin J. vandeVen
    Pages 69-97
  5. Victor Chen
    Pages 99-109
  6. H. Ernst Keller
    Pages 111-126
  7. Carol J. Cogswell, Kieran G. Larkin
    Pages 127-137
  8. Jonathan Art
    Pages 183-196
  9. Hans Chen, Jason R. Swedlow, Marcus Grote, John W. Sedat, David A. Agard
    Pages 197-210
  10. Roger Y. Tsien, Alan Waggoner
    Pages 267-279
  11. P. C. Cheng, A. Kriete
    Pages 281-310
  12. Robert Bacallao, Kianush Kiai, Lynn Jesaitis
    Pages 311-325
  13. M. Terasaki, M. E. Dailey
    Pages 327-346

About this book


This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.


Confocal Microscopy Pawley biological biology cell biology fluorescence information instruments laser lens optics photon quality system

Editors and affiliations

  • James B. Pawley
    • 1
  1. 1.Zoology DepartmentUniversity of Wisconsin-MadisonMadisonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4757-5348-6
  • Copyright Information Springer-Verlag US 1995
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4757-5350-9
  • Online ISBN 978-1-4757-5348-6
  • About this book
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