Biochemical and Clinical Applications of Titration Calorimetry and Enthalpimetric Analysis

  • Albert C. Censullo
  • John A. Lynch
  • Dan H. Waugh
  • Joseph Jordan


Several analytical methods based on adiabatic reaction calorimetry have been developed in recent years. Authoritative reviews of thermometric enthalpy titrations (including kinetic titrimetry), direct injection enthalpimetry and thermokinetic analysis are available.1–8 Applications to biochemical and clinical analysis8–20 have thus far been sporadic notwithstanding the fact that these fields are in dire need of instrumental methods. The relevant fundamental phenomenon (enthalpy change) is universal for all chemical reactions including biochemically and clinically important processes. Successful studies of biologically significant oxidation-reductions by titration calorimetry are reported in this paper. A new method, called “peak enthalpimetry,” is described and assessed in the context of contemporary requirements for instrumental approaches to clinical analysis. The use of novel Curie point temperature sensors for immunological determinations is discussed.


Axial Ligand Carrier Stream Mercuric Nitrate Chloride Determination Integral Plot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References Cited

  1. 1.
    L.S. Bark, S.M. Bark, Thermometric Titrimetry, Pergamon Press, Oxford, 1969.Google Scholar
  2. 2.
    L.S. Bark, P. Bate, J.K. Grime, “Thermometric and Enthalpimetric Titrimetry” in Selected Annual Reviews of the Analytical Society, Vol. II (L.S. Bark, Ed.) Society for Analytical Chemistry, London, 1972, pp. 121–149.Google Scholar
  3. 3.
    P.W. Carr, Crit. Rev. of Anal. Chem., 3, 491 (1972).CrossRefGoogle Scholar
  4. 4.
    R. M. Izatt, J.J. Christensen, “Thermochemistry in Inorganic Solution Chemistry,” in Physical Methods in Advanced Inorganic Chemistry, H.A.O. Hill, P. Day, Eds., Interscience, New York, 1968, Chap. 11, pp. 538–598.Google Scholar
  5. 5.
    J. Jordan, “Thermometric Enthalpy Titrations” in Treatise in Analytical Chemistry, I.M. Kolthoff, P.J. Elving, Eds., Part I, Vol. 8, Interscience, New York, 1968, pp. 5175–5242.Google Scholar
  6. 6.
    J. Jordan, in Topics in Chemical Instrumentation, G. W. Ewing, Ed., Chemical Education Publ. Co., Easton, Pa., 1971, pp. 193–199.Google Scholar
  7. 7.
    H. J. V. Tyrell, A. E. Beezer, Thermometric Titrimetry, Chapman and Hall, London, 1968.Google Scholar
  8. 8.
    J. Jordan, N.D. Jespersen, Coll. Int. Cent. Nat. Rech. Sci., 201, 59 (1972).Google Scholar
  9. 9.
    E. B. Smith, P. W. Carr, Anal. Chem., In Press (1974).Google Scholar
  10. 10.
    J.J. Christensen, R.M. Izatt, D.P. Wrathall, L. Hansen, J. Chem. Soc. A., 1212 (1969).Google Scholar
  11. 11.
    J.J. Christensen, J.L. Oscarson, R.M. Izatt, J. Am. Chem. Soc, 90, 5949 (1968).CrossRefGoogle Scholar
  12. 12.
    J.J. Christensen, J.H. Rytting, R.M. Izatt, Biochemistry, 9 , 4907 (1970).CrossRefGoogle Scholar
  13. 13.
    J.J. Christensen, J.H. Rytting, R.M. Izatt, J. Chem. Soc. B., 1643 (1970).Google Scholar
  14. 14.
    J.J. Christensen, J.H. Rytting, R.M. Izatt, J. Chem. Soc. B., 1646 (1970).Google Scholar
  15. 15.
    J.J. Christensen, M.D. Slade, D.M. Smith, R.M. Izatt, J. Tsang, J. Am. Chem. Soc, 92, 4164 (1970).CrossRefGoogle Scholar
  16. 16.
    R.M. Izatt, J.J. Christensen, J.H. Rytting, Chem. Rev., 71, 439 (1971).CrossRefGoogle Scholar
  17. 17.
    R.M. Izatt, J.J. Christensen, Handbook of Biochemistry, H.A. Sober, Ed., Chemical Rubber Co., Cleveland, 1968, np.J-49.Google Scholar
  18. 18.
    N.D. Jespersen, J. Jordan, Anal. Letters, 3, 323 (1970).CrossRefGoogle Scholar
  19. 19.
    M.A. Marini, R.L. Berger, D.P. Lan, C.J. Martin, Anal. Biochem., 43, 188 (1971).CrossRefGoogle Scholar
  20. 20.
    B. Sen, W.C. Wu, Anal. Chim. Acta, 2, 457 (1971).Google Scholar
  21. 21.
    R.J. Thompson, Thesis in Progress, Pennsylvania State University.Google Scholar
  22. 22.
    D.H. Waugh, Thesis in Progress, Pennsylvania State University.Google Scholar
  23. 23.
    P. Priestley, W. Sebborn, R. Selman, Analyst, 90, 598 (1965).CrossRefGoogle Scholar
  24. 24.
    W. McLean, G. Penketh, Talanta, 15, 1185 (1968).CrossRefGoogle Scholar
  25. 25.
    T. Crompton, B. Cope, Anal. Chem., 40 , 274 (1968).CrossRefGoogle Scholar
  26. 26.
    H. Yoshida, M. Taga, S. Hikime, Japan Analyst, 20 , 361 (1971).CrossRefGoogle Scholar
  27. 27.
    J.G. Montalvo, D.G. Davis, J. Electroanal. Chem., 23, 164 (1969).CrossRefGoogle Scholar
  28. 28.
    A.C. Censullo, Thesis in Progress, Pennsylvania State UniversityGoogle Scholar
  29. 29.
    M.L. McGlashan, Pure and Appl. Chem., 21, 3 (1970).CrossRefGoogle Scholar
  30. 30.
    W. Mansfield Clark, Oxidation-Reduction Potentials of Organic Systems, Williams and Wilkins Co., Baltimore, 1960, p. 318.Google Scholar
  31. 31.
    Lowell J. Reed, J. Berkson, J. Phys. Chem., 33, 760 (1929).CrossRefGoogle Scholar
  32. 32.
    S.J. Ashcroft, CT. Mortimer, Thermochemistry of Transition Metal Complexes, Academic Press, New York, 1970, p. 276.Google Scholar
  33. 33.
    O. Schales, S. Schales, J. Biol. Chem., 140, 879 (1941).Google Scholar
  34. 34.
    R.J.P. Williams, J. Phys. Chem., 58, 121 (1954).CrossRefGoogle Scholar
  35. 35.
    T.M. Bednarski, J. Jordan, J. Am. Chem. Soc, 89, 1552 (1967).CrossRefGoogle Scholar
  36. 36.
    C. K. Chang, T.G. Traylor, J. Am. Chem. Soc, 95, 5810 (1973).CrossRefGoogle Scholar
  37. 37.
    CK. Chang, T.G. Traylor, Proc. Nat. Acad. Sci. U.S.A., 70, 2647 (1973).CrossRefGoogle Scholar
  38. 38.
    J.E. Baldwin, Joel Huff, J. Am. Chem. Soc, 95, 5757 (1973).CrossRefGoogle Scholar
  39. 39.
    N.D. Jespersen, Thesis, Pennsylvania State University, 1971.Google Scholar
  40. 40.
    J.A. Lynch, Thesis in Progress, Pennsylvania State University.Google Scholar
  41. 41.
    J.A. Lynch, J. Jordan, Abstracts, 25th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Cleveland, 1974, Paper #54.Google Scholar

Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • Albert C. Censullo
    • 1
  • John A. Lynch
    • 1
  • Dan H. Waugh
    • 1
  • Joseph Jordan
    • 1
  1. 1.Department of Chemistry, 152 Davey LaboratoryThe Pennsylvania State UniversityUniversity ParkUSA

Personalised recommendations