Use of LLC-PK1 Monolayers as an In Vitro Model for Nephrotoxicity
When cell cultures are used for in vitro nephrotoxicity studies, the cells are usually grown on a solid surface, which allows only apical treatment with xenobiotics. However, in vivo, xenobiotics can gain access to the proximal tubule cell from the apical as well as from the basolateral side. Functional differences exist between the apical and basolateral membranes, especially with regard to transport phenomena, and thus different mechanisms may underlie a possible nephrotoxicity. To study these mechanisms in vitro, the cells should have the same functional differences between apical and basolateral membranes and should be grown as a confluent monolayer on a porous support, to allow both apical and basolateral exposure.
KeywordsBasolateral Membrane Proximal Tubule Cell Basolateral Side Mercapturic Acid Apical Treatment
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- 5.J.J.W.M. Mertens, J.G.J. Weijnen, W.J. van Doorn, A. Spenkelink, J.H.M. Tenmink and P.J. van Bladeren, Differential toxicity as a result of apical and basolateral treatment of LLC-PK1 monolayers with S-(1,2,3,4,4-penta-chlorobutadienyl)glutathione and N-acetyl- S-(1,2,3,4,4-pentachlorobutadi-enyl)-L-cysteine, submitted.Google Scholar