Nephrotoxicity pp 337-342 | Cite as

Alpha-Methylglucose Uptake by Isolated rat Kidney Proximal Tubular Cells as a Parameter for Cell Integrity in Vitro

  • P. J. Boogaard
  • G. J. Mulder
  • J. F. Nagelkerke


A large number of nephrotoxins cause damage to the proximal tubule in vivo. Many typical in vivo tubular cell functions are conserved in freshly isolated rat kidney cells (Ormstad et al., 1981; Jones et al., 1979). An advantage of the use of isolated cells as compared to the in vivo experiment is that it permits a defined quantitatively and qualitatively cellular environment, which allows study of the relationship between the concentration of a nephrotoxin, exposure time and effect. Extra-renal effects can also be excluded, thus isolated tubular cells are very suitable to study the effects of nephrotoxins which exert their effect directly at the tubular site.


Platinum Luminal HEPES Collagenase EGTA 
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  1. Albrechtsson U., Hultberg B., Lârusdóttir H. and Norgren L., 1985, Nephrotoxicity of ionic and non-ionic contrast media in aortofemoral angiography, Acta Radiol. Diagnosis, 26, 615–.618Google Scholar
  2. Aull J.L., Rice C. and Tebbets L.A., 1977, Interactions of platinum complexes with the essential and non-essential sulfhydryl groups of thymidylate synthetase, Biochemistry, 16, 672–677PubMedCrossRefGoogle Scholar
  3. Howe-Grant M.E. and Lippard S.J. , 1980, Aqueous platinum (II) chemistry; binding to biological molecules. In: “Metal ions in Biological systems”, Vol. 11: “Metal complexes as anticancer agents”, Sigel H. ed., Marcel Dekker, New YorkGoogle Scholar
  4. Jones D.P ., Sundby G.-B., Ormstad K. and Orrenius S., 1979, Use of isolated kidney cells for study of drug metabolism, Biochem. Pharmacol., 29, 929–935CrossRefGoogle Scholar
  5. Kimmich G.A., Randles J. and Brand J.S., 1975, Assay of picomole amounts of ATP, ADP, and AMP using the luciferase enzyme system, Anal. Biochem., 69, 187–206PubMedCrossRefGoogle Scholar
  6. Leonard B.J., Eccleston E., Jones D., Todd P. and Walpole A., 1979, Antileukemic and nephrotoxic properties of platinum compounds, Nature, 234, 43–45CrossRefGoogle Scholar
  7. Lowry O.H., Rosebrough N.J., Farr A.L. and Randau R.J., 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265–275PubMedGoogle Scholar
  8. McGuire J.P., Friedmann M.E. and McAuliffe C.A., 1984, Studies of enzyme inhibition. The interaction of some platinum (II) complexes with fumarase and malate dehydrogenase, Inorg. Chim. Acta, 91, 161–165CrossRefGoogle Scholar
  9. Ormstad K., Orrenius S. and Jones D.P., 1981, Preparation and characteristics of isolated kidney cells, Meth. Enz., 77, 137–146CrossRefGoogle Scholar
  10. Rutenberg A.M., Kim H., Fishbein J.W., Hanker J.S., Wasserberg H.L. and Seligman A.M., 1969, Histochemical and structural demonstration of gammaglutamyltranspeptidase activity, J. Histochem. Cytochem, 17, 517–525CrossRefGoogle Scholar
  11. Silvermann M., 1976, Glucose transport in the kidney, Biochem. Biophys. Acta. 457, 303–351CrossRefGoogle Scholar
  12. Silvermann M., 1986, Comparison of glucose transport mechanisms at opposing surfaces of the renal proximal tubular cell, Biochem. Cell. Biol., 64, 1092–1098CrossRefGoogle Scholar
  13. Soltoff S.P., 1986, ATP and the regulation of renal cell function, Ann. Rev. Phvs., 48, 9–31CrossRefGoogle Scholar
  14. Ullrich K.J., 1986, Polarity of the proximal tubular cell: comparison of luminal and contraluminal transport systems for hexoses, dicarboxylates and sulfate, In: “Endocrine regulation electrolyte balance,” Krueck F. and Thurau K. eds. p.28–35, Springer Verlag, HeidelbergCrossRefGoogle Scholar
  15. Von Hoff D.D., Schilsky R., Reichert C.M., Reddick R.L., Rozencweig M., Young R.C. and Muggis F.M., 1979, Toxic effects of cis-dichlorodiammineplatinum (II) in man, Canc. Tr. Rep., 63, 1527–1531Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • P. J. Boogaard
    • 1
  • G. J. Mulder
    • 1
  • J. F. Nagelkerke
    • 1
  1. 1.Division of Toxicology, Center for Bio-Pharmaceutical SciencesUniversity of LeidenLeidenThe Netherlands

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