Electrophoretic Separation of Normal and Mutant Phosphoribosylpyrophosphate Synthetase

  • P. Boer
  • O. Sperling
  • A. de Vries
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41 B)

Abstract

Phosphoribosylpyrophosphate (PRPP) synthetase (E.C. 2.7.6.1) catalyzes the formation of PRPP from ribose-5-phosphate and ATP in the presence of Mg++ and inorganic phosphate. The product, PRPP, is a substrate of the first rate limiting step of the de novo synthesis of purine nucleotides and its availability has been shown to regulate this pathway in human tissue (1). A superactive mutant erythrocyte PRPP synthetase with decreased sensitivity to feedback inhibition has recently been found by us in a gouty family (2,3).

Keywords

Cellulose Acetate Electrophoretic Separation Liver Extract Purine Nucleotide First Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Fox I.H. and Kelley W.N. Ann. Intern. Med. 74:424, 1971.PubMedCrossRefGoogle Scholar
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    Sperling O., Boer P., Persky-Brosh S., Kanarek E. and De Vries A. Europ. J. Clin. Biol. Res. 17:703, 1972.Google Scholar
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    Sperling O., Persky-Brosh S., Boer P. and De Vries A. Bioch. Medicine, In press, 1973.Google Scholar
  4. 4.
    Fox I.H. and Kelley W.N. J. Biol. Chem. 246:5739, 1971.PubMedGoogle Scholar
  5. 5.
    Sperling O., Boer P., Persky-Brosh S. and De Vries A. FEBS letters, 27:229, 1972.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • P. Boer
    • 1
  • O. Sperling
    • 1
  • A. de Vries
    • 1
  1. 1.Rogoff-Wellcome Medical Research Institute and the Metabolic Unit of Department of Medicine DTel-Aviv University Medical School, Beilinson HospitalPetah TikvaIsrael

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