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Hapten — Sandwich Labeling of Cell-Surface Antigens

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Contemporary Topics in Molecular Immunology

Part of the book series: Contemporary Topics in Molecular Immunology ((CTI))

Abstract

The indirect antibody technique for amplified detection of antigens (Coons, 1956) has a wide and expanding range of applications. In general, this well-established method employs second-layer antiimmunoglobulin, coupled to an appropriate detector, to label first-layer antibody specific for a particular antigen. The last few years have seen the introduction of hapten-sandwich labeling procedures (Lamm et al,1972; Wofsy et al.,1974; Cammisuli and Wofsy, 1976) that circumvent the requirement of the conventional sandwich technique for antibodies from one species directed against the immunoglobulins of another. Hapten-sandwich labeling uses instead detector-coupled antihapten antibodies to amplify first-layer antibodies to which the hapten has been covalently attached.

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Abbreviations

ars:

P-azobenzene

benz:

p-azobenzoate

BzPen:

benzylpenicilloyl

Con A:

concanavalin A

DHB:

methyl 3,5-dihydroxybenzimidate

DNP:

2,4-dinitrophenyl

FACS:

fluorescence-activated cell sorter

FcR:

Fc receptors

FI:

fluorescein

gal:

p-azophenyl ß-D galactoside

glut:

benzoyl-L-glutamic acid

HB:

methyl p-hydroxybenzimidate

KLH:

keyhole limpet hemocyanin

lac:

p-azophenyl ß D lactoside

NIP:

4-hydroxy-3-iodo-5-nitrophenylacetyl

PBS:

0.01 M phosphate buffer containing 8.5 mg/m1 NaCI

PFC:

plaque-forming cells

Rh:

rhodamine

SRBC:

sheep red blood cells

sulf:

p-azobenzene sulfonate

TMA:

p-azotrimethylanilinium

TNP:

2,4, 6-tinitrophenyl

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Author information

Authors and Affiliations

  1. Department of Bacteriology and Immunology, University of California, Berkeley, California, 94720, USA

    Leon Wofsy & Claudia Henry

  2. Basel Institute for Immunology, Basel, Switzerland

    Salvatore Cammisuli

Authors
  1. Leon Wofsy
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  2. Claudia Henry
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  3. Salvatore Cammisuli
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Editor information

Editors and Affiliations

  1. Department of Molecular Immunology, Scripps Clinic and Research Foundation, La Jolla, California, USA

    Ralph A. Reisfeld

  2. Department of Biochemistry, East Tennessee State University, Johnson City, Tennessee, USA

    F. P. Inman

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Wofsy, L., Henry, C., Cammisuli, S. (1978). Hapten — Sandwich Labeling of Cell-Surface Antigens. In: Reisfeld, R.A., Inman, F.P. (eds) Contemporary Topics in Molecular Immunology. Contemporary Topics in Molecular Immunology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0779-3_7

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  • DOI: https://doi.org/10.1007/978-1-4757-0779-3_7

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  • Online ISBN: 978-1-4757-0779-3

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