Hapten — Sandwich Labeling of Cell-Surface Antigens

  • Leon Wofsy
  • Claudia Henry
  • Salvatore Cammisuli
Part of the Contemporary Topics in Molecular Immunology book series (CTI)


The indirect antibody technique for amplified detection of antigens (Coons, 1956) has a wide and expanding range of applications. In general, this well-established method employs second-layer antiimmunoglobulin, coupled to an appropriate detector, to label first-layer antibody specific for a particular antigen. The last few years have seen the introduction of hapten-sandwich labeling procedures (Lamm et al,1972; Wofsy et al.,1974; Cammisuli and Wofsy, 1976) that circumvent the requirement of the conventional sandwich technique for antibodies from one species directed against the immunoglobulins of another. Hapten-sandwich labeling uses instead detector-coupled antihapten antibodies to amplify first-layer antibodies to which the hapten has been covalently attached.


Hapten Group Biotin Hydrazide Avidin Molecule Conventional Sandwich Trinitrobenzene Sulfonate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this chapter







Con A

concanavalin A


methyl 3,5-dihydroxybenzimidate




fluorescence-activated cell sorter


Fc receptors




p-azophenyl ß-D galactoside


benzoyl-L-glutamic acid


methyl p-hydroxybenzimidate


keyhole limpet hemocyanin


p-azophenyl ß D lactoside




0.01 M phosphate buffer containing 8.5 mg/m1 NaCI


plaque-forming cells




sheep red blood cells


p-azobenzene sulfonate




2,4, 6-tinitrophenyl


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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Leon Wofsy
    • 1
  • Claudia Henry
    • 1
  • Salvatore Cammisuli
    • 2
  1. 1.Department of Bacteriology and ImmunologyUniversity of CaliforniaBerkeleyUSA
  2. 2.Basel Institute for ImmunologyBaselSwitzerland

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