The Effect of Antigen on Antibodies: Recent Studies

  • Henry Metzger
Part of the Contemporary Topics in Molecular Immunology book series (CTI)


In 1972–1973 I reviewed what was known about the effect of antigen on the properties of antibodies (Metzger, 1974). Several possibilities which had been suggested by various investigators were considered. According to the allosteric model, antigen by virtue of its chemical structure induces conformational changes distal to the combining site. These changes would in turn lead to changes in the molecule (receptor) which was activated by the antigen-antibody complex. According to the distortive model, the topological distribution of antigenic determinants leads to a distortion of a semirigid antibody molecule, resulting in effects similar to those postulated for the allosteric model. Finally a simple associative model of antigen action was considered. Here polymerization of the antibody by a multideterminant antigen would be the critical and perhaps sufficient role played by antigen. I concluded that “... the weight of evidence speaks against both the allosteric and distortive models for antigen action. We are left with the associative model. I know of no data that are inconsistent with this model ... and there is much evidence in favor of it.”


Electron Spin Resonance Spin Label Hinge Region Complement Fixation Site Saturation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allan, R., and Isliker, H., 1974a, Immunochemistry 7: 175.Google Scholar
  2. Allan, R., and Isliker, H., 1974b, Immunochemistry 11: 243.Google Scholar
  3. Amzel, L. M., Poljak, R. J., Saul, F., Varga, J., and Richards, F. F., 1974, Proc. Nat. Acad. Sci. USA 71: 1427.PubMedGoogle Scholar
  4. Arlaud, G. J., Meyer, C. M., and Colomb, M. G., 1976, FEBS Lett. 66: 132.PubMedGoogle Scholar
  5. Assimeh, S. N., and Painter, R. H., 1975, J. Immunol. 115: 488.Google Scholar
  6. Barisas, B. G., Singer, S. J., and Sturtevant, J. M., 1977, Immunochemistry 14: 247.PubMedGoogle Scholar
  7. Beal, D., and Feinstein, A., 1976, Q. Rev. Biophys. 9: 135.Google Scholar
  8. Bigelow, C. C., Smith, B. R., and Dorrington, K. F., 1974, Biochemistry 13: 4602.PubMedGoogle Scholar
  9. Brown, J. C., and Koshland, M. E., 1975, Proc. Nat. Acad. Sci. USA 72: 5111.PubMedGoogle Scholar
  10. Brown, J. C., and Koshland, M. E., 1977, Proc. Nat. Acad. Sci. USA 74: 5682.Google Scholar
  11. Bunting, D. S., Kells, D. I. E., Kortan, C., and Dorrington, K. J., 1977, Immunochemistry 14: 45.PubMedGoogle Scholar
  12. Burton, D. R., Dwek, R. A., Forsén, S., and Kariström, G., 1977a, Biochemistry 16: 250.PubMedGoogle Scholar
  13. Burton, D. R., Forsén, S., Karlström, G., Dwek, R. A., McLaughlin, A. C., and Wain-Hobson, S., 1977b, Eur. J. Biochem. 75: 445.PubMedGoogle Scholar
  14. Cathou, R. E., and Dorrington, K. J., 1975, in Subunits in Biological Systems (S. N. Timasheff and G. D. Fasman, eds.), Vol. 7., Part C, Marcel Dekker, New York, p. 91.Google Scholar
  15. Cerottini, J. C., and Fitch, F., 1968, Int. Arch. Allergy Appl. Immunol. 34: 188.PubMedGoogle Scholar
  16. Chan, L. M., and Cathou, R. E., 1977, J. Mol. Biol. 112: 653.Google Scholar
  17. Charlwood, P. A., and Utsumi, S. 1969, Biochem. J. 112: 357.PubMedGoogle Scholar
  18. Cohen, S., and Becker, E. L., 1968, J. ImmunoL 100: 403.Google Scholar
  19. Cohn, M., 1971, Ann. N.Y. Acad. Sci. 190: 529.PubMedGoogle Scholar
  20. Colman, P. M., Deisenhofer, J., Huber, R., Haupt, H., and Schwick, G., 1976, J. Mol. Biot 100: 257.Google Scholar
  21. Colomb, M. G., and Porter, R. R., 1975, Biochem. J. 145: 177.PubMedGoogle Scholar
  22. Connell, G. E., and Porter, R. R., 1971, Biochem. J. 124: 53 P.Google Scholar
  23. Coutinho, A., and Möller, G., 1975, Adv. Immunol. 21: 114.Google Scholar
  24. Crothers, D. M., and Metzger, H., 1972, Immunochemistry 9: 341.PubMedGoogle Scholar
  25. Cunniff, R. V., and Stollar, B. D., 1968, J. Immunol. 100: 7.PubMedGoogle Scholar
  26. Czer, L., Gladkikh, I. A., Kozlov, Zh. A., Nezlin, R. S., Ogievetskaya, M. M., and Ostanevich, Y. M., 1976, FEBS Lett. 68: 283.Google Scholar
  27. Czer, L., Franek, F., Gladkikh, I. A., Nezlin, R. S., Novotny, J., and Ostanevich, Y. M., 1977, FEBS Lett. 80: 329.Google Scholar
  28. Davies, D. R., Padlan, E. A., and Segal, D. M., 1975, Ann. Rev. Biochem. 44: 639.PubMedGoogle Scholar
  29. DeLisi, C., and Thakur, A. K., 1977, Cell Immunol. 28: 416.PubMedGoogle Scholar
  30. Deisenhofer, J., Colman, P. M., Epp, O., and Huber, R., 1976, Hoppe-Seyler’s Physiol. Chem. 357: 1421.Google Scholar
  31. Dintzis, H. M., Dintzis, R. Z., and Vogelstein, B., 1976, Proc. Nat. Acad. Sci. USA 10: 3671.Google Scholar
  32. Dorrington, K. J., and Bennich, H., 1973, J. Biot Chem. 248: 8378.Google Scholar
  33. Dorrington, K. J., Bennich, H., and Turner, M. W., 1972, Biochem. Biophys. Res. Commun. 47: 512.PubMedGoogle Scholar
  34. Dower, S. K., Dwek, R. A., McLaughlin, A. C., Mole, L. E., Press, E. M., and Sunderland, C. A., 1975, Biochem. J. 149: 73.PubMedGoogle Scholar
  35. Dower, S. K., Wain-Hobson, S., Gettins, P., Givol, D., Jackson, W. R. C., Perkins, S. J., Sunderland, C. A., Sutton, B. J., Wright, C. E., and Dwek, R. A., 1977, Biochem. J. 165: 207.PubMedGoogle Scholar
  36. Dwek, R. A., Jones, R., Marsh, D., McLaughlin, A. C., Press, E. M., Price, N. C., and White, A. I., 1975, Phil. Trans. R. Soc. (London) Ser. B 272: 53.Google Scholar
  37. Edberg, C. S., Bronson, P. M., and Van Oss, C. J., 1972, Immunochemistry 9: 273.PubMedGoogle Scholar
  38. Edelman, G. M., Cunningham, B. A., Gall, W. E., Gottliev, P. D., Rutishauser, V., and Waxdal, M. J., 1969, Proc. Nat. Acad. Sci. USA 63: 78.PubMedGoogle Scholar
  39. Edmundson, A. B., Ely, K. R., Girling, R. L., Abola, E. E., Schiffer, M., and Westholm, F. A., 1974, Progress in Immunology—II (L. Brent and J. Holborow, eds.), Vol. 1, p. 103, North-Holland, New York.Google Scholar
  40. Ellerson, J. R., Yasmeen, D., Painter, R. H., and Dorrington, K. J., 1972, FEBS Lett. 24: 318.PubMedGoogle Scholar
  41. Ellerson, J. R., Yasmeen, D., Painter, R. H., and Dorrington, K. J., 1976, J. Immunol. 116: 510.PubMedGoogle Scholar
  42. Feinstein, A. and Beal, D., in: Immunochemistry (M. W. Steward and L. E. Glynn, eds.), Wiley and Sons, Inc., London.Google Scholar
  43. Feinstein, A., Munn, E. A., and Richardson, N. E., 1971, Ann. N.Y. Acad. Sci. USA 190: 104.Google Scholar
  44. Fett, J. W., Deutsch, H. W., and Smithies, D. E., 1973, Immunochemistry 10: 115.PubMedGoogle Scholar
  45. Fujio, H., and Karush, F., 1966, Biochemistry 5: 1856.PubMedGoogle Scholar
  46. Fujita, T., Takiuchi, M., Iida, K., Nagaki, K., and Inai, S., 1977, Immunochemistry 14: 25.PubMedGoogle Scholar
  47. Froese, A., and Sehon, A. H., 1975, in: Contemporary Topics in Molecular Immunology (F. P. Inman, ed.), Vol. 4, p. 23, Plenum, New York.Google Scholar
  48. Givol, D., Pecht, I., Hochmann, J., Schlessinger, J., and Steinberg, I. Z., 1974, Proc. Sec. Int. Congr. Immunol. 1: 39.Google Scholar
  49. Goers. J. W.. Schumaker, V. N., Glovsky, M. M., Rebek, J., and Müller-Eberhard, H. J., 1975, J. Biol. Chem. 250: 4918.PubMedGoogle Scholar
  50. Goers, J. W., Ziccardi, R. J., Schumaker, V. N., and Glovsky, M. M., 1977, J. Immunol. 118: 2182.PubMedGoogle Scholar
  51. Götze, O., and Müller-Eberhard, H. J., 1976, Adv. Immunol. 24: 1.PubMedGoogle Scholar
  52. Hamburger, R. N., 1975, Science 189: 389.PubMedGoogle Scholar
  53. Hoffmann, L.G., 1976a, Immunochemistry 13: 725.PubMedGoogle Scholar
  54. Hoffmann, L. G., 1976b, Immunochemistry 13: 731.PubMedGoogle Scholar
  55. Hoffmann, L. G., 1976c, Immunochemistry 13: 737.PubMedGoogle Scholar
  56. Holowka, D. A., and Cathou, R. E., 1976a, Biochemistry 15: 3373.PubMedGoogle Scholar
  57. Holowka, D. A., and Cathou, R. E., 1976b, Biochemistry 15: 3379.PubMedGoogle Scholar
  58. Huber, R., Deisenhofer, J., Colman, P. M., Matsushima, M., and Palm, W., 1976, Nature 264: 415.PubMedGoogle Scholar
  59. Humphries, G. M. K., and McConnell, H. M., 1977, Proc. Nat. Acad. Sci. USA 74: 3537.PubMedGoogle Scholar
  60. Hurst, M. M., Volonakis, J., Hester, R. B., Stroud, R. M., and Bennett, J. C., 1975, J. Exp. Med. 140: 1117.Google Scholar
  61. Hurwitz, E., Zatz, M. M., and Segal, D. M., 1977,J. Immunol. 118:1348.Google Scholar
  62. Inbar, D., Hochman, J., and Givol, D., 1972, Proc. Nat. Acad. Sci. USA 69: 2659.PubMedGoogle Scholar
  63. Isacki, S., and Raynaud, M., 1972, Ann. Inst. Pasteur. 123: 695.Google Scholar
  64. Isenman, D. E., Dorrington, K. J., and Painter, R. N., 1975a, J. Immunol. 114: 1726.PubMedGoogle Scholar
  65. Isenman, D. E., Painter, R. H., and Dorrington, K. J., 1975b, Proc. Nat. Acad. Sci. USA 72: 548.Google Scholar
  66. Isenman, D. E., Ellerson, J. R., Painter, R. H., and Dorrington, K. J., 1977, Biochemistry 16: 233.PubMedGoogle Scholar
  67. Isersky, C., Taurog, J. D., Poy, G., and Metzger, H., 1978,J. Immunol. (in press). Ishizaka, K., Ishizaka, T., and Banovitz, J., 1964,J. Immunol. 93:1001.Google Scholar
  68. Ishizaka, K., Ishizaka, T., and Banovitz, J., 1965, J. Immunol. 94: 824.Google Scholar
  69. Ishizaka, T., Tada, T., and Ishizaka, K., 1968, J. Immunol. 100: 1145.Google Scholar
  70. Ishizaka, T., Chang, T. H., Taggart, M., and Ishizaka, K., 1977, J. Immunol. 119: 1589.Google Scholar
  71. Jaton, J.-C., Huser, H., Blath, Y., and Pecht, I., 1975a, Biochemistry 14: 5308.PubMedGoogle Scholar
  72. Jaton, J.-C., Huser, H., Braun, D. G., Givol, D., Pecht, I. and Schlessinger, J., 1975b, Biochemistry 14: 5312.PubMedGoogle Scholar
  73. Jaton, J.-C., Huser, H., Riesen, W. F., Schlessinger, J. and Givol, D., 1976, J. Immunol. 116: 1363.PubMedGoogle Scholar
  74. Jolley, M. E., Rudikoff, S., Potter, M., and Glaudemans, C. P. J., 1973, Biochemistry 12: 3039.PubMedGoogle Scholar
  75. Jolley, M. E., Glaudemans, C. P. J., Rudikoff, S., and Potter, M., 1974, Biochemistry 13: 3179.PubMedGoogle Scholar
  76. Käiväräinen, A. I., and Nezlin, R. S., 1976a, Immunochemistry 13: 1001.PubMedGoogle Scholar
  77. Käiväräinen, A. I., and Nezlin, R. S., 1976b, Biochem. Biophys. Res. Commun. 68: 270.Google Scholar
  78. Käiväräinen, A. I., Nezlin, R. S., Lichtenstein, G. I., Mischarin, A. Y., and Volkenstein, M. V., 1973a, Ma Biol. USSR 7: 760.Google Scholar
  79. Käiväräinen, A. I., Nezlin, R. S., and Volkenstein, M. V., 1973b, FEBS Lett. 35: 306.PubMedGoogle Scholar
  80. Käiväräinen, A. I., Nezlin, R. S., and Volkenstein, M. V., 1974, Mol. Biol. USSR 8: 816.Google Scholar
  81. Kakimoto, K., and Onoue, K., 1974, J. Immunol. 112: 1373.Google Scholar
  82. Karlsson, F. A., 1972, J. Immunol. 109: 110.Google Scholar
  83. Kehoe, J. M., and Fougereau, M., 1969, Nature 224: 1212.PubMedGoogle Scholar
  84. Kehoe, J. M., Bourgois, A., Capra, J. D., and Fougereau, M., 1974, Biochemistry 13: 2499.PubMedGoogle Scholar
  85. Kelly, K. A., Sehon, A. H., and Froese, A., 1971, Immunochemistry 8: 613.PubMedGoogle Scholar
  86. Kishimoto, T., and Ishizaka, K., 1975, J. Immunol. 114: 585.PubMedGoogle Scholar
  87. Kuo, C. Y., Thompson, J. T., and Hoffmann, L. G., 1976, Z. Immunitaetsforsch. 152: 151.Google Scholar
  88. Lancet, D., and Pecht, I., 1976, Proc. Nat. Acad. Sci. USA 73: 3549.PubMedGoogle Scholar
  89. Lapanje, S., and Dorrington, K. J., 1973, Biochem. Biophys. Acta 322: 45.PubMedGoogle Scholar
  90. Levine, B. B., 1965, J. Immunol. 94: 121.Google Scholar
  91. Lopes, A. D., and Steiner, L. A., 1973, Fed. Proc. 32: 1003.Google Scholar
  92. Low, Y. L. K., Liu, Y.-S. V., and Putman, F. W., 1976, Science 191: 390.PubMedGoogle Scholar
  93. McCammon, J. A., and Karplus, M., 1977, Nature 268: 765.PubMedGoogle Scholar
  94. Metzger, H., 1970, Adv. Immunol. 12: 69.Google Scholar
  95. Metzger, H., 1974, Ad v. Immunol. 18: 164.Google Scholar
  96. Metzger, H., 1977, in: Receptors and Recognition (P. Cuatrecasas and M. F. Greaves, eds.), Ser. A, p. 75, Chapman and Hall, London.Google Scholar
  97. Michaelson, T. E., 1976, Scand. J. Immunol. 5: 1123.Google Scholar
  98. Michaelson, T. E., Frangione, B., and Franklin, E. C., 1977, J. Biol. Chem. 252: 883.Google Scholar
  99. Milstein, C., and Frangione, B., 1971, Biochem. J. 121: 217.PubMedGoogle Scholar
  100. Monod, J., Wyman, J., and Changeaux, J. P., 1965, J. Mol. Biol. 12: 88.PubMedGoogle Scholar
  101. Mukkur, T. K. S., and Fang, W. D., 1977, Immunochemistry 14: 63.PubMedGoogle Scholar
  102. Nezlin, R. S., Zagyansky, Y. A., Käiväräinen, A. I., and Stefani, D. V., 1973, Immunochemistry 10: 681.PubMedGoogle Scholar
  103. Nezlin, R. S., Timofeeev, V. P.,Sykulev, Y. K., and Zurabjan, S. E., 1977, Immunochemistry (in press).Google Scholar
  104. Nisonoff, A., Hopper, J. E., and Spring, S. B., 1975, The Antibody Molecule, Academic Press, New York.Google Scholar
  105. Ovary, Z., Saluk, P. H., Quijada, L., and Lamm, M. E., 1976, J. Immunol. 116: 1265.PubMedGoogle Scholar
  106. Padlan, E. A., 1977, Q. Rev. Biophys. 10: 35.PubMedGoogle Scholar
  107. Padlan, E. A., Segal, D. M., Spande, T. F., Davies, D. R., Rudikoff, S., and Potter, M., 1973, Nature (New Biol.) 245: 165.Google Scholar
  108. Pecht, I., 1976, in: Recognition and Allostery in the Mechanism of Antibody Action (F. Melchers and K. Rajewsky, eds.), p. 41, Springer-Verlag, Berlin.Google Scholar
  109. Pecht, I., and Lancet, D., 1976, in: Chemical Relaxation in Molecular Biology ( I. Pecht and R. Rigler, eds.), Springer-Verlag, Heidelberg.Google Scholar
  110. Pecht, I., Ehrenberg, E., Calef, E., and Arnon, R., 1977, Biochem. Biophys. Res. Commun. 74: 1302.PubMedGoogle Scholar
  111. Pilz, I., Puchwein, G., Kratky, O., Herbst, M., Haager, O., Gall, W. E., and Edelman, G. M., 1970, Biochemistry 9: 211.PubMedGoogle Scholar
  112. Pilz, I., Kratky, O., Licht, A., and Sela, M., 1973, Biochemistry 12: 4998.PubMedGoogle Scholar
  113. Pilz, I., Kratky, O., and Karush, F., 1974, Eur. J. Biochemistry 41: 91.Google Scholar
  114. Pilz, I., Kratky, O., Licht, A., and Sela, M., 1975, Biochemistry 14: 1326.PubMedGoogle Scholar
  115. Pilz, I., Schwartz, E., and Palm, W., 1976, Eur. J. Biochemistry 71: 239.Google Scholar
  116. Pilz, I., Schwartz, E., and Palm, W., 1977, Eur. J. Biochemistry 75: 195.Google Scholar
  117. Poljak, R. J., 1975, Adv. Immunol. 21: 1.PubMedGoogle Scholar
  118. Pollet, R., and Edelhoch, H., 1973, J. Biol. Chem. 248: 5443.PubMedGoogle Scholar
  119. Pollet, R., Ede1hoch, H., Rudikoff, S., and Potter, M., 1974, J. Biol. Chem. 248: 5443.Google Scholar
  120. Putnam, F. W., Florent, G., Paul, C., Shiruoda, T., and Shimizo, A., 1973, Science 182: 287.PubMedGoogle Scholar
  121. Reid, K. B. M., 1971, Immunology 20: 649.PubMedGoogle Scholar
  122. Reid, K. B. M., Sim, R. B., and Faiers, A. P., 1977, Biochem. J. 161: 239.PubMedGoogle Scholar
  123. Romans, D. G., Tilley, C. A., Crookston, M. C., Falk, R. E., and Dorrington, K. J., 1977, Proc. Nat. Acad. Sci. USA 74: 2531.PubMedGoogle Scholar
  124. Sandberg, A. L., Oliveira, B., and Osler, A. G., 1971a, J. Immunol. 106: 282.PubMedGoogle Scholar
  125. Sandberg, A. L., Göetze, O., Müller-Eberhard, H. J., and Osier, A. G., 1971b, J. Immunol. 107: 920.PubMedGoogle Scholar
  126. Schlessinger, J., Steinberg, I. Z., Givol, D., Hochman, J., and Pecht, I., 1975, Proc. Nat. Acad. Sci. USA 72: 2775.PubMedGoogle Scholar
  127. Schur, P., and Becker, E. L., 1963, J. Exp. Med. 118: 891.Google Scholar
  128. Segal, D. M., and Hurwitz, E., 1976, Biochemistry 15: 5253.PubMedGoogle Scholar
  129. Segal, D. M., and Hurwitz, E., 1977, J. Immunol. 118: 1338.Google Scholar
  130. Segal, D. M., Taurog, J. D., and Metzger, H., 1977, Proc. Nat. Acad. Sci. USA 74: 2993.PubMedGoogle Scholar
  131. Sharon, J., and Givol, D., 1976, Biochemistry 15: 1591.PubMedGoogle Scholar
  132. Silverton, E. W., Navia, M. A., and Davies, D. R., 1977, Proc. Nat. Acad. Sci. USA 74: 5140.PubMedGoogle Scholar
  133. Siraganian, R. P., Hook, W. A., and Levine, B. B., 1975, Immunochemistry 12: 149.Google Scholar
  134. Skubitz, K. M., O’Hara, D. S., and Smith, T. W., 1977, J. Immunol. 118: 1971.Google Scholar
  135. Smith, B. R., and Dorrington, K. J., 1972, Biochem. Biophys. Res. Commun. 46: 1061.PubMedGoogle Scholar
  136. Solheim, B. G., and Harboe, M., 1972, Immunochemistry 9: 623.PubMedGoogle Scholar
  137. Stewart, G. A., Smith, A. K., and Stanworth, D. R., 1973, Immunochemistry 10: 755.PubMedGoogle Scholar
  138. Stewart, G. A., Johnson, P. M., Barrett, M. W., Scopes, P. M., and Stanworth, D. R., 1977, Immunochemistry 14: 263.PubMedGoogle Scholar
  139. Sutton, B. J., Gettins, P., Givol, D., Marsh, D., Wain-Hobson, S., Willan, K. J., and Dwek, R. A., 1977, Biochem. J. 165: 177.PubMedGoogle Scholar
  140. Thompson, J. J., and Hoffmann, L. G., 1974a, Immunochemistry 11: 431.PubMedGoogle Scholar
  141. Thompson, J. J., and Hoffmann, L. G., 1974b, Immunochemistry 11: 537.PubMedGoogle Scholar
  142. Unkeless, J. C., 1977, J. Exp. Med. 145: 931.PubMedGoogle Scholar
  143. Vardinon, N., Spirer, Z., Fridkin, M., Schwartz, J., and Ben-Efraim, S., 1977, Acta Allergol. 32: 291.PubMedGoogle Scholar
  144. Venyaminov, S. Y., Rajnavölgyi, E., Medgyesi, G. A., Gergely, J., and Zâvodszky, P., 1976, Eur. J. Biochem. 67: 81.PubMedGoogle Scholar
  145. Wahl, P., 1969, Biochem. Biophys. Acta 175: 55.PubMedGoogle Scholar
  146. Wain-Hobson, S., Dower, S. K., Gettins, P., Givol, D., McLaughlin, A. C., Pecht, I., Sunderland, C. A., and Dwek, R. A., 1977, Biochem. J. 165: 227.PubMedGoogle Scholar
  147. Wasserman, E., and Levine, L., 1960, J. Immunol. 87: 290.Google Scholar
  148. Willan, K. J., Marsh, D., Sunderland, C. A., Sutton, B. J., Wain-Hobson, S., Dwek, R. A., and Givol, D., 1977a, Biochem. J. 165: 199.PubMedGoogle Scholar
  149. Willan, K. J., Wallace, K. H., Jaton, J.-C., and Dwek, R. A., 1977b, Biochem. J. 161: 205.PubMedGoogle Scholar
  150. Willan, K. J., Golding, B., Givol, D., and Dwek, R. A., 1977c, FEBS Lett. 80: 133.PubMedGoogle Scholar
  151. Yasmeen, D., Ellerson, J. R., Dorrington, K. J., and Painter, R. H., 1976, J. Immunol. 116: 518.PubMedGoogle Scholar
  152. Yguerabide, J., Epstein, H. F., and Stryer, L., 1970, J. Mol. Biol. 51: 573.PubMedGoogle Scholar
  153. Zagyansky, Y. A., 1973, FEBS Lett. 35: 309.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Henry Metzger
    • 1
  1. 1.Arthritis and Rheumatism Branch National Institute of Arthritis, Metabolism and Digestive DiseasesNational Institutes of HealthBethesdaUSA

Personalised recommendations