Abstract
Peripheral blood cells from 9 E- and 6 E+ SIg- ALL patients and from 11 CML patients in TdT+ blastic crisis were subjected to discontinuous albumin gradient separation according to Dicke’s method, and TdT assayed in the six fractions recovered therefrom. The major results were: (i) in E- ALL and in CML-BC, TdT+ cells could be recovered either within a narrow range of specific densities or were spread over most of the gradient, which might suggest differences in cell maturation among the patients; (ii) in some instances, most leukemic blasts were found in TdT-negative or faintly positive fractions; (iii) in most, but not all, E- ALL and BC, the majority of TdT+ cells was found in low density fractions; (iv) all E+ ALL had high density TdT+ cells. Cell fractions of most patients were also examined at the electron microscope, and correlations between morphology and marker characterization were tentatively drawn.
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Marini, M., Bagnara, G.P., Biagini, G., Baccarani, M., Rosito, P. (1982). TdT-Positive and TdT-Negative Human Leukemic Cells: Specific Density and Morphology. In: Bertazzoni, U., Bollum, F.J. (eds) Terminal Transferase in Immunobiology and Leukemia. Advances in Experimental Medicine and Biology, vol 145. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8929-3_29
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DOI: https://doi.org/10.1007/978-1-4684-8929-3_29
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