Abstract
Neutrophils are the cells generally considered to be the first line of defence against invading microorganisms. Stimulation of their surface receptors activates a repertoire of killing functions as phagocytosis, superoxide production, exocytosis of acid hydrolases and proteases, chemotaxis, aggregation and generation of arachidonic acid-derived inflammatory mediators. The major microbicidal mechanisms of the human granulocytes(PMN) require the formation of reactive oxygen metabolites such as superoxide anion (02) and hydrogen peroxide (the oxidative burst) and the secretion of granule enzymes such as myeloperoxidase (degranulation). Both of these events can be triggered by various soluble or particulate stimuli which interact with the cell surface and depolarize the cell membranes. The PMN to exposed to the specific murine monoclonal antibodies (MoAbs) exhibit a significant depression in (02) generation in response to fMLP to serum-treated zymosan (STZ). Preliminary data suggested that MoAbs depolarizes the PMN cell membrane and that this may be associated with the alterations in oxidative metabolism caused by same MoAbs2’3. The purpose of this study was to explore the relationship between surface membrane glycoproteins, membrane depolarization in the human granulocytes and the subsequent oxidative burst Several MoAbs were used for to identifie the membrane glycoprotein fundamental in respiratory burst activation.
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References
Goetzl E.J., Goldstein I.M., Cellular components of
inflammation: granulocytes.In “TEXTBOOK OF
RHEUMATOLOGY“ pg.115–144 eds by Kelley W.N.,Harris E.D.,Ruddy S.,Sledge C.B.,pbl by W.B. Saunders Company Philadelphia (1985).
Mauri C.,Rizzo S.C., Ricevuti G. eds. “The Biology of Phagocytes in Health and Disease”, Advances in biosciences series, vol.66, Pergamon Press (1987).
Martin M.A., Nauseef W.M., Clark R.A.,Depolarization Blunts the oxidative burst of human neutrophils. J Immuno1. 140: 3928 (1988).
Babior D.M., Kipness R.S.,.Curnutte J.I.Biological
defence mechanisms: the production by leukocytes of superoxide,a potential bactericidal agent. J.Clin. Invest. 52:741 (1973).
Bellavite P.,The superoxide-forming enzymatic system of phagocytes Free Radical Biology & Medicine 4:225 (1988).
Cross C.E., Oxygen Radicals and Human Disease Ann. Int. Med. 107:526 (1987).
Klebanoff S. J.. Oxigen metabolism and the toxic properties of phagocytes Ann.Int.Med.93:48O (1980).
Koren H.S., Proposed classification of leukocyte-associated cytolytic molecules Immunologz Today 8:139 (1987).
Ricevuti G. Mazzone A. The neutrophils revisited. Inflammation 13, 475, (1989).
Ricevuti G. Mazzone A. Harlan JH. Membrane glycoproteins and related disease. Haematologica 73: 415 (1988).
Solomon SS,Palazzolo M. Activation of cyclic AMP phosphodiesterase by phorbol and protein kinase C pathway.Am J. Med.Sci. 292: 182(1986).
Sedor JR.Free radicals and prostanoid synthesis J Lab Clin Med 108:521 (1986).
Willis H.E., Browder B., Feister A.J., Mohanakumar T. Ruddy S.Monoclonal antibody to human IgG Fc receptorsJ Tmmunol 140: 234 (1988)
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© 1990 Plenum Press, New York
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Ricevuti, G., Mazzone, A., Notario, A. (1990). Membrane Glycoproteins in Superoxide Release from Neutrophils. In: de Paulet, A.C., Douste-Blazy, L., Paoletti, R. (eds) Free Radicals, Lipoproteins, and Membrane Lipids. NATO ASI Series, vol 189. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7427-5_5
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DOI: https://doi.org/10.1007/978-1-4684-7427-5_5
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