Abstract
The onset of the liver fatty degeneration which follows rat acute poisoning with carbon tetrachlorip(CC14) is provoked by a severe block of lipoprotein secretory pathway.1,2,3 Such a complex functional impairment is already relevant few hours after treatment and probably involves various cellular compartments. Previous studies employing the isolated hepatocyte model gave indirect indications for an early involvement of liver Golgi apparatus in the CC14-induced block of intracellular transport of the lipoprotein micelles.4,5 More recent studies with rat hepatocytes in single cell suspension have allowed us to directly demonstrate a marked impairment of lipoprotein maturation and transport at the Golgi level soon after CC14 treatment.6 “In vivo” investigations of the changes occurring in the Golgi apparatus during the oxidative stress due to CC14 are here reported. In addition, the analysis of the pathogenesis of these Golgi disturbances has been attempted by using a strong antioxidant treatment represented by rat supplementation with large doses of alpha-tocopherol prior to CC14 dosing.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
T. F. Slater, Hepatotoxicity of carbon tetrachloride: fatty degeneration, in: “Free Radical Mechanisms in Tissue Injury,” J. R. Lagnado, ed., Pion Ltd., London (1972).
M. U. Dianzani, Biochemical aspects of fatty liver, in: “Biochemical Mechanisms of Liver Injury,” T. F. Slater, ed., Academic Press, London (1978).
W. J. Brattin, E. A. Glende Jr, and R. O. Recknagel, Pathological mechanisms in carbon tetrachloride hepatotoxicity, J. Free Rad. Biol. Med. 1: 27 (1985).
E. Gravela, E. Albano, M. U. Dianzani, G. Poli, and T. F. Slater, Effects of carbon tetrachloride on isolated rat hepatocytes, Biochem. J. 178: 509 (1979).
G. Poli, E. Gravela, E. Albano, and M. U. Dianzani, Studies on fatty liver with isolated hepatocytes, Exp. Mol. Pathol. 30: 116 (1979).
G. Poli, E. Chiarpotto, E. Albano, D. Cottalasso, G. Nanni, U. M. Marinari, A. M. Bassi, and M. U. Dianzani, Carbon tetrachloride-induced inhibition of hepatocyte lipoprotein secretion: functional impairment of Golgi apparatus in the early phases of such injury, Life Sci. 36: 533 (1985).
P. M. Pfeifer, and P. B. McCay, Reduced triphosphopyridine nucleotide oxidase-catalyzed alterations of membrane phospholipids, J. Biol. Chem. 246: 6401 (1971).
U. M. Marinari, D. Cottalasso, G. R. Gambella, M. M. Averame, M. A.ronzato, and G. Nanni, Effects of acute ethanol intoxication on H-palmitic acid transport through hepatocyte Golgi apparatus,FEBS Lett. 86: 53 (1978).
J. H. Ehrenreich, J. J. M. Bergeron, P. Siekevitz, and G. E. Palade, Golgi fractions prepared from rat liver homogenates, J. Cell Biology 59: 45 (1973).
G. Poli, K. H. Cheeseman, T. F. Slater, and M. U. Dianzani, The role of lipid peroxidation in CC14-induced damage to liver microsomal enzymes: comparative studies in vitro using microsomes and isolated liver cells, Chem.-Biol. Interactions 37: 13 (1981).
C. de Duve, B. C. Pressman, R. Gianetto, R. Wattiaux, and F. Appelmans F, Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue, Biochem. J. 60: 604 (1955).
Z. Sellinger, H. Beauf ay, A. Jacques, A. Doyeu, and C. de Duve, Intracellular distribution and properties of beta-N-acetylglucosaminidase and beta-galactosidase in rat liver, Biochem. J. 74: 450 (1960).
F. M. Baccino, G. A. Rita, and M. F. Zuretti, Studies on the structure-bound sedimentability of some rat liver lysosome hydrolases, Biochem. J. 122: 363 (1971).
G. L. Peterson, A simplification of the protein assay method of Lowry et al. which is more generally applicable, Anal. Biochem. 83: 346 (1977).
B. Fleischer, S. Fleischer, and H. Ozawa, Isolation and characterization of Golgi membrane from bovine liver, J. Cell Biology 43: 59 (1969).
T. F. Slater, and B. C. Sawyer, The stimulatory effects of carbon tetrachloride on peroxidative reactions in rat liver fractions “in vitro”, Biochem. J. 123: 815 (1971).
G. W. Burton, A. Webb, and K. U. Ingold, A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios, Lipids 20: 29 (1985).
U. M. Marinari, M. A. Pronzato, D. Cottalasso, A. Zicca-Cadoni, G. Nanni, G. Poli, E. Chiarpotto, E. Albano, F. Biasi, and M. U. Dianzani, CC14-induced early functional impairment of rat liver Golgi apparatus, in: “Free Radicals in Liver Injury,” G. Poli, K. H. Cheeseman, M. U. Dianzani, and T. F. Slater, eds., IRL Press, Oxford (1985).
G. Poli, E. Albano, F. Biasi, G. Cecchini, R. Carini, G. Bellomo, and M. U. Dianzani, Lipid peroxidation stimulated by carbon tetrachloride or iron and hepatocyte death: protective effect of vitamin E, in: “Free Radicals in Liver Injury,” G. Poli, K. H. Cheeseman, M. U. Dianzani, and T. F. Slater, eds., IRL Press, Oxford (1985).
F. P. Coronglu, M. Lai, and A. Milia, Carbon tetrachloride, bromotrichloromethane and ethanol acute intoxication, Biochem. J. 212: 625 (1983).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this chapter
Cite this chapter
Poli, G. et al. (1990). Carbon Tetrachloride-Induced Oxidative Stress at the Level of Liver Golgi Apparatus: Effect on Lipoprotein Secretion. In: de Paulet, A.C., Douste-Blazy, L., Paoletti, R. (eds) Free Radicals, Lipoproteins, and Membrane Lipids. NATO ASI Series, vol 189. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7427-5_18
Download citation
DOI: https://doi.org/10.1007/978-1-4684-7427-5_18
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4684-7429-9
Online ISBN: 978-1-4684-7427-5
eBook Packages: Springer Book Archive