Abstract
5-Phosphoribosyl 1-pyrophosphate (PRPP) is a primary substrate in the de novo and salvage pathways of the biosyntheses of purine, pyrimidine, and pyridine nucleotides [1]. It also serves as a regulatory substrate for amidophosphoribosyltransferase and as a critical activator for carbamoylphosphate synthetase II [2], key enzymes of the purine and pyrimidine de novo syntheses, respectively. In man, abnormalities of this enzyme were reported, which causes hyperuricemia and eventually gout [3–7], This disease was transmitted as an X-linked trait, and, in fact, this PRPP synthetase gene was mapped to the human chromosome Xq22–q26, using human enzyme-specific antibody [8]. Recently we showed that rat PRPP synthetase has two distinct types of subunits, referred to as PRS I and PRS II, by cDNA cloning [9] and N-terminal amino acid sequencing [K. Kita et al., in this volume]. Studies with human and mouse somatic cell hybrids showed that human PRS I and PRS II genes were both located on different regions of the human chromosome X [10]. Superactivity of PRPP synthetase was categorized into five groups by Becker et al. [11]. However, the complex cases were reported: for instance, B-lymphoblast lines derived from individuals with the excessive maximal reaction velocity (catalytic defects) of this enzyme in erythrocytes, fibroblasts, and lymphocytes do not express the superactive phenotype [12]. This phenomenon may be caused by the presence of the two genes for this enzyme. For the precise analysis of the human PRPP synthetase genes and their abnormalities, we have attempted to clone cDNAs of the human enzyme. In this paper, we reported the cloning of cDNA for human PRPP synthetase subunit II and the deduced amino acid sequence.
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References
I.H. Fox and W.N. Kelly, Phosphoribosylpyrophosphate in man: biochemical and clinical significance. Ann. Intern. Med. 74: 424 (1971).
M. Tatibana and K. Shigesada, Control of pyrimidine biosynthesis in mammalian tissues. V. Regulation of glutamine-dependent carbamyl-phosphate synthetase: activation by 5-phosphoribosyl 1-pyrophosphate and inhibition by uridine triphosphate, J. Biochem. 72: 549 (1972).
O. Sperling, P. Boer, S. Perskey-Brosh, E. Kanarek, and A. de Vries, Altered kinetic property of erythrocyte phosphoribosylpyrophosphate synthetase in excessive purine overproduction. Rev. Eur. Etud. Clin. Biol. 17: 703 (1972).
M.A. Becker, L.J. Meyer, A.W. Wood, and J.E. Seegmiller, Purine overproduction in man associated with increased phosphoribosylpyrophosphate synthetase activity, Science 179: 1123 (1973).
M.A. Becker, Patterns of phosphoribosylpyrophosphate and ribose-5-phosphate concentration and generation in fibroblasts from patients with gout and purine overproduction, J. Clin. Invest. 57: 308 (1976).
M.A. Becker, K.O. Raivio, B. Bakay, W.B Adams, and W.L. Nyhan, Variant human phosphoribosylpyrophosphate synthetase altered in regulatory and catalytic functions, J. Clin. Invest. 65: 109 (1980).
I. Akaoka, S. Fujimori, N. Kamatani, F. Takeuchi, E. Yano, Y. Nishida, A. Hashimoto, and Y. Horiuchi, A gouty family with increased phosphoribosylpyrophosphate synthetase activity: case reports, familial studies, and kinetic studies of the abnormal enzyme, J. Rheumatol. 8: 563 (1981).
M.A. Becker, R.C.K. Yen, P. Itkin, S.J. Gross, J.E. Seegmiller, and B. Bakay, Regional localization of the gene for human phosphoribosylpyrophosphate synthetase on the X-chromosome, Science 203: 1013 (1979).
M. Taira, S. Ishijima, K. Kita, K. Yamada, T. Iizasa and M. Tatibana, Nucleotide and deduced amino acid sequences of two distinct cDNAs for rat phosphoribosylpyrophosphate synthetase, J. Biol. Chem. 262: 14867 (1987).
M. Taira, J. Kudoh, S. Minoshima, Y. Shimizu, M. Tatibana, and N. Shimizu, Chromosomal localization of the human genes for phosphoribosylpyrophosphate synthetase subunits I and II, Human Gene Mapping Workshop 9: 739 (1987).
M.A. Becker, M.J. Losman, J. Wilson, and H.A. Simmonds, Superactivity of human phosphoribosylpyrophosphate synthetase due to altered regulation by nucleotide inhibitors and inorganic phosphate, Biochim. Biophys. Acta 882: 168 (1986).
M.J. Losman, D. Rimon, M. Kim, and M.A. Becker, Selective expression of phosphoribosylpyrophosphate synthetase superactivity in human lymphoblast lines, J. Clin. Invest. 76: 1657 (1985).
J.M. Chirgwin, A.E. Przybyla, R.J. MacDonald, and W.J. Rutter, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease, Biochemistry 18: 5294 (1979).
T. Maniatis, E.F. Fritsch, and J. Sambrook, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982).
T.V. Huynh, R.A. Young, and R.W. Davis, Constructing and screening cDNA libraries in λgt10 and λgt11, in: “DNA cloning: a practical approach,” D.M. Glover, ed., Vol. 1, p. 49, IRL Press, Washington, D.C. (1985).
F. Sanger, S. Nicklen, and A.R. Coulson, DNA sequencing with chain terminating inhibitors, Proc. Natl. Acad. Sci. USA. 74: 5463 (1977).
M.O. Dayhoff, R.M. Schwartz, and B.C. Orcutt, A model of evolutionary change in proteins, in: “Atlas of Protein Sequence and Structure,” Vol. 5, Suppl. 3, p. 345, National Biomedical Research Foundation, Washington, D.C. (1978).
J.C.A. Bardwell and E.A. Craig, Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous, Proc. Natl. Acad. Sci. USA. 81: 848 (1984).
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© 1989 Plenum Press, New York
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Iizasa, T., Taira, M., Shimada, H., Tatibana, M. (1989). Deduced Amino Acid Sequence from Human Phosphoribosylpyrophosphate Synthetase Subunit II cDNA. In: Mikanagi, K., Nishioka, K., Kelley, W.N. (eds) Purine and Pyrimidine Metabolism in Man VI. Advances in Experimental Medicine and Biology, vol 253A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5673-8_84
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DOI: https://doi.org/10.1007/978-1-4684-5673-8_84
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