Abstract
The last two steps of de novo synthesis of UMP are catalyzed by the sequential actions of orotate phosphoribosyltransferase <1> (orotate PRTase; pyrophosphate phosphoribosyltransferase; EC 2.4.2.10) and orotidine 5′-monophosphate decarboxylase <2> (OMP decarboxylase; orotidine 5′-phosphate carboxy-lyase; EC 4.1.1.23)
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References
R.W. Mclard, M.J. Black, L.R. Livingstone and M.E. Jones, Isolation and initial characterization of the single polypeptide that synthesizes uridine 5′-monophosphate from orotate in Ehrlich ascites carcinoma. Purification by tandem affinity chromatography of uridine-5′-monophosphate synthase, Biochemistry 19: 4699 (1980)
L.R. Livingstone and M.E. Jones, The purification and preliminary characterization of UMP synthase from human placenta, J. Biol. Chem. 262: 15726 (1987)
W.N. Kelley, Hereditary orotic aciduria, in “The Metabolic Basis of Inherited Disease 5th ed,” J.B. Stanbury, J.B. Wyngaarden, D.S. Fredrickson, J.L. Goldstein, eds., McGraw-Hill, New York (1983)
M.E. Perry and M.E. Jones, Studies of UMP synthase in orotic aciduria fibroblasts, Fed. Proc. Fed. Am. Soc. Exp. Biol. 46: 309 (1987)
M. Rose, P. Grisafi and D. Botstein, Structure and function of the yeast URA3 gene: expression in Escherichia coli, Gene 29: 113 (1984)
C.-A. Ohmstede, S.D. Langdon, C.-B. Chae and M.E. Jones, Expression and sequence analysis of a cDNA encoding the orotidine-5′-monophosphate decarboxylase domain from Ehrlich ascites uridylate synthase, J. Biol. Chem. 261: 4276 (1986)
W.D. Benton and R.W. Davis, Screening gt recombinant clones by hybridization to single plaques in situ, Science 196: 180 (1977)
T. Maniatis, E.F. Fritsch and J. Sambrook (eds), “Molecular Cloning,” Cold Spring Harbor, New York (1982)
F. Sanger, J. Nicklen and A.R. Coulson, DNA sequencing with chainterminating inhibitors, Pro, Natl. Acad. Sci. U.S.A. 74: 5463 (1977)
M.E. Jones, P.R. Kavipurapu and T.W. Traut, Orotate phosphoribosyl-transferase: Orotidylate decarboxylase (Ehrlich ascites cell), Methods Enzymol. 51: 155 (1978)
M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72: 248 (1976)
J.M. Chirgwin, A.E. Przybyla, R.J. MacDonald and W.J. Rutter, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease, Biochemistry 18: 5294 (1979)
D. Werner, Y. Chemla and M. Herzberg, Isolation of poly(A) RNA by paper affinity chromatography, Anal. Biochem. 141: 329 (1984)
P.S. Thomas, Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose, Proc. Natl. Acad. Sci. U.S.A. 77: 5201 (1980)
M. Suchi, Molecular genetic studies on hereditary orotic aciduria. Purification of human orotidine 5′-monophosphate decarboxylase and cloning of its cDNA, Nagoya Med. J. 32: 207 (1988)
N.J. Proudfoot and G.G. Brownlee, 3′ non-coding region sequences in eukaryotic messenger RNA, Nature 263: 211 (1976)
M. Kozak, Point mutations define a sequence flanking the AUG initiation codon that modulated translation by eukaryotic ribosomes, Cell 44: 283 (1986)
D.P. Suttle, B.Y. Bugg, J.K. Winkler and J.J. Kanalas, Molecular cloning and nucleotide sequence for the complete coding region of human UMP synthase, Proc. Natl. Acad. Sci. U.S.A. 85: 1754 (1988)
H. Morishita, M. Kokubo, S. Sumi, M. Suchi and Y. Wada, The first case of hereditary orotic aciduria in Japan, J. Japan Pediatr. Soc. 90: 2775 (1986)
M. Yazaki, K. Okajima, M. Suchi, H. Morishita and Y. Wada, Increase of protein synthesis by uridine supplement in lectin-stimulated peripheral blood lymphocytes and EB virus transformed B cell line of hereditary orotic aciduria, Tohoku J. Exp. Med. 153: 189 (1987)
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© 1989 Plenum Press, New York
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Suchi, M. et al. (1989). Molecular Cloning of Human UMP Synthase. In: Mikanagi, K., Nishioka, K., Kelley, W.N. (eds) Purine and Pyrimidine Metabolism in Man VI. Advances in Experimental Medicine and Biology, vol 253A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5673-8_83
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DOI: https://doi.org/10.1007/978-1-4684-5673-8_83
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