Abstract
Antisense oligonucleotides with base sequence complementary to a specific RNA or DNA seem to provide a promising new tool for the therapy of viral diseases and of cancer. They offer the exciting potential of selectively modulating the expression of an individual gene (Hélène and Toulmé, 1990). The oligonucleotides have been used successfully in cell culture to inhibit the expression of specific HIV genes (Agrawal et al, 1988; Goodchild et al, 1988; Lisziewicz et al, 1988) and there is now widespread interest in extending the use of antisense compounds in the in vivo setting (Bayever et al, 1993; Wahlestedt et al, 1993). However, before the therapeutic potential of antisense compounds can be fullfilled, it will be necessary to overcome significant problems relating to their poor stability and their ability to reach their intracellular target.
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References
Agrawal, S., Ikeuchi, T., Sun, D., Sarin, P.S., Konopka, A., Maizel, T. and Zamecnick, P.C., 1988, Inhibition of human immunodeficiency virus in early infected and chronically infected cells by antisense oligodeoxynucleotides and their phosphorothioate analogues, Proc.Natl.Acad.Sci. USA, 86:7790
Agrawal, S., Goodchild, J., Civeira, M.P., Thornton, A.H., Sarin, P.S. and Zamecnik, P.C., 1988, Oligodeoxyribonucleoside phosphoramidates and phosphorothioates as inhibitors of human immunodeficiency virus, Proc.Natl.Acad.Sci. USA, 85:7079
Aguilar, J.J., Anel, A., Torres, J.M., Semmel, M. and Uriel, J., 1991, Changes in lipid composition of human peripheral blood lymphocytes infected by HIV, AIDS Res. and Human Retroviruses. 7:761
Akhtar, S., Kole, R. and Juliano, R.L., 1991, Stability of modified antisense oligodeoxyribonucleotides in cellular extracts and sera, Life Sci., 49:1793
Allen T.M., Hong, K. and Papahadjopoulos, D., 1990, Membrane contact, fusion, and hexagonal HII transitions in phosphatidylethanolamine liposomes, Biochemistry, 29:2976
Alving, C.R., 1988, Macrophages as targets of liposome-encapsulated antimicrobial agents, Adv. Drug Delivery Rev., 2:107
Apostolov, K., Barker, W., Galpin, S.A., Habib, N.A., Wood, C.B. and Kinchington, D., 1989, Syncitia formation in HIV-1 infected cells is associated with an increase in cellular oleic acid, FEBS Lett., 250:241
Bayever, E., Iversen, P.L., Bishop, M.R., Sharp, J.G., Tewary H.K., Arneson, M.A., Pirrucello, S.J., Ruddon, R.W., Kessinger A., Zon, G. and Armitage J.O., 1993, Systemic administration of a phosphorothioate oligonucleotide with a sequence complementary to p53 for acute myelogenous leukemia and myelodysplastic syndrome: initial results of a phase I trial, Antisense Res.Dev., 3:383
Bazile, D., Mir, L.M. and Paoletti, C., 1989, Voltage-dependent introduction of a octothymidylate into electropermeabilized cells, Biochem.Biophvs.Res.Comm., 159:633
Bentz, J., Ellens, H., Lai, M.Z. and Szoka, F.C., 1985, On the correlation between HII phase and the contact induced destabilization, Proc.Natl.Acad.Sci. USA, 82:5742
Cecarelli, B., Huribut, W.P. and Mauro, A., 1972, Depletion of vesicles from frog neuromuscular junctions by prolonged tetanic stimulation, J. Cell. Biol., 23:85
Chu, C.J., Dijkstra, J., Lai, M.Z., Hong, K. and Szoka, F.C., 1990, Efficiently of cytoplasmic delivery by pH-sensitive liposomes to cells in culture, Pharm.Res., 7:824
Connor, J., Yatvin, M.B. and Huang, L., 1984, pH-sensitive liposomes: Acid-induced liposome fusion, Proc.Natl.Acad.Sci. USA, 81:1715
Connor, J. and Huang, L., 1985, Efficient cytoplasmic delivery of a fluorescent dye by pH-sensitive liposomes, J. Cell Biol., 101:582
Connor, J., Yatvin, M.B. and Huang, L., pH-sensitive liposomes: Acid-induced liposome fusion, Proc.Natl.Acad.Sci., USA, 81:1715
Dash, P., Lotan, L., Knapp, M., Kandel, E.R. and Goelet, P., 1987, Selective elimination of mRNAs in vivo: complementary oligonucleotide promote RNA degradation by an RNaseH like activity, Proc.Natl.Acad.Sci. USA, 84:7896
Düzgünes, N., Straubinger, R.M., Baldwin, P.A., Friend, D.S. and Papahadjopoulos, D., 1985, Proton induced fusion of oleic acid-phosphatidylethanolamine Liposomes, Biochemistry, 24:3091
Goodchild, J., Agrawal, S., Civeira, M.P., Sarin, P.S., Sun, D. and Zamecnik, P.C., 1988, Inhibition of replication and expression of human T cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotide complementary to viral RNA, Proc.Natl.Acad.Sci. USA, 85:5507
Hélène, C. and Toulmé, J.J., 1990, Specific regulation of gene expression by antisense, sense and antigene nucleic acids, Biochim.Biopphys. Acta., 1049:99
Holtzman, E., Freeman, A.R. and Cashner, A., 1971, Stimulation dependent alteration in peroxidase uptake at loster neuromuscular junctions, Science, 173:733
Leonetti, J.P., Machy, P., Degols, G., Lebleu, B. and Leserman, L., 1990, Antibody-targeted liposomes containing oligodeoxyribonucleotides complementary to viral DNA selectively inhibit viral replication, Proc.Natl.Acad.Sci. USA, 87:2448
Lisziewicz, J., Sun, D., Metelev, V., Zamecnick, P.C., Gallo, R.C., and Agrawal, S., 1988, Long-term treatment of human immunodeficiency virus-infected cells with antisense oligonucleotide phosphorothioates, Proc.Natl.Acad.Sci. USA, 90:3860
Litzinger, D.C. and Huang, L., 1992, Phosphatidylethanolamine liposomes: drug delivery, gene transfer and immunodiagnostic applications, Biochim.Biophys. Acta, 1113:201
Liu, D. and Huang, L., 1989, Role of cholesterol in the stability of pH-sensitive liposomes prepared by the detergent dialysis method, Biochem.Biophys. Acta., 981:254
Liu, D. and Huang, L., 1990, pH-sensitive, plasma-stable liposomes with relatively prolonged residence in circulation, Biochem.Biophys.Acta, 1022:348
Marsh, M. and Helenius, A., 1989, Virus entry into animal cells, Advanced in Virus Research, 36:107
Ropert, C., Okruszek, A., Couvreur, P. and Malvy, C., 1994, Intracellular stability of antisense oligonucleotides determines their induced inhibition of a retrovirus, Antisense Research and Development, 4:207
Ropert, C., Lavignon, M., Dubernet, C., Couvreur, P. and Malvy, C., 1992, Oligonucleotides encapsulated in pH-sensitive liposomes are efficient towards the Friend retrovirus, Biochem.Biophys.Res.Comm. 183:879
Ropert, C., Malvy, C. and Couvreur, P., 1993, Inhibition of the Friend retrovirus by antisense oligonucleotides encapsulated in liposomes: Mechanism of action, Pharm. Res., 10:1427
Ropert, C., Mishal, Z., Rodrigues, J.M., Jr, Malvy, C. and Couvreur P., Retrovirus budding may constitute a port of entry for drug carriers, Bioch.Biophys. Acta, in press
Steiman, R.M., Mellman, I.S., Muller, W.A. and Cohn, Z.A., 1983, Endocytosis and the recycling of plasma membrane, J. Cell. Biol., 96:1
Sullivan, S.M., Gieseler, R.K.H., Lenzner, S., Ruppert, J., Gabrysiak, T.G., Peters, J.H., Cox, G., Richer, L., Martin, W.J. and Scolaro, M.J., 1992, Inhibition of human immunodeficiency virus I proliferation by liposome-encapsulated sense DNA to the 5’ TAT splice acceptor site, Antisense Res.Develop., 2:187
Thierry, A.R., Rahman, A. and Dritschilo A., Liposomal delivery as a new approach to transport antisense oligonucleotides, in: Gene regulation, Biology of Antisense RNA and DNA, Eds, R.P. Erickson and J.G. Izant, Raven Press Ltd, New york, 1992, pp.147–161
Thierry, A.R. and Dritschilo A., 1992, Intracellular availibility of unmodified phosphorothioated and liposomally encapsulated oligonucleotides for antisense activity, Nucleic Acid Res., 20:5691
Thierry, A.R., Rahman, A. and Dritshilo, A., 1993, Overcoming multidrug resistance in human tumor cells using free and liposomally encapsulated oligonucleotides, Biochem. Biophvs.Res.Commun., 190:952
Uhlmann, E. and Peyman, A., 1990, Antisense oligonucleotides: A new therapeutic principle, Chem.Rev., 90:544
Wahlestedt C., Golanov E., Yamamoto S., Yee F., Ericson H. and Yoo H., Inumisi C.E., Reis, D.J., 1993, Antisense oligodeoxyribonucleotides to NMDA-R1 receptor channel protect cortical neurons from excitotoxicity and reduce focal ischaemic infarctions. Nature, 363:260
Wang, C.-Y. and Huang, L., 1989, Highly efficient transfection mediated by pH sensitive immunoliposomes, Biochemistry, 28:9508
Wickstrom, E., 1986, Oligodeoxyribonucleotide stability in subcellular extracts and culture media, J. Biochem. Biophvs. Methods, 13:97
Zelphati, O., Zon, G. and Leserman, L., 1993, Inhibition of HIV replication with antisense oligonucleotides encapsulated in immunoliposomes, Antisense Res.Develop., 3:323
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Ropert, C., Malvy, C., Couvreur, P. (1996). pH Sensitive Liposomes as Efficient Carriers for Intracellular Delivery of Oligonucleotides. In: Gregoriadis, G., McCormack, B. (eds) Targeting of Drugs 5. NATO ASI Series, vol 290. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6405-8_16
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DOI: https://doi.org/10.1007/978-1-4615-6405-8_16
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