Reactive Oxygen Species in Normal Physiology, Cell Injury and Phagocytosis

  • Mary Treinen Moslen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 366)


This presentation will examine major processes by which biological systems cope with reactive oxygen species (ROS). Peroxidation of membrane lipids will be considered as one example of the destructive processes that occur when ROS are not controlled by the protection systems that detoxify reactive species. In contrast, enzymatic peroxidation of arachidonic acid initiates the biosynthesis of chemotactic leukotrienes. Phagocytic cell formation of ROS by the “oxidative burst” will be examined because of its important role in the defense against invading organisms. The focus will be on the processes that stringently control activation of the NADPH oxidase of phagocytic cells.


Reactive Oxygen Species NADPH Oxidase Oxidative Burst Chronic Granulomatous Disease Phagocytic Cell 
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  1. Alin, P., Danielson, U.H., and Mannervik, B. 1985, 4-Hydroxyalk-2-enals are substrates for glutathione transferase. Proc. Meet. Fed. Eur. Biochem. Soc. 179:267.CrossRefGoogle Scholar
  2. Babior, B.B. 1984, Oxidant from phagocytes: Agents of defense and destruction. Blood 64:959.PubMedGoogle Scholar
  3. Buettner, G.R. 1993, The pecking order of free radicals and antioxidants: lipid peroxidation, α-tocopherol, and ascorbate. Arch. Biochem. Biophys. 300:535.PubMedCrossRefGoogle Scholar
  4. Cantin, A.M., North, S.L., Hubbard, R.C., and Crystal, R.C. 1987, Normal alveolar epithelial lining fluid contains high levels of glutathione. J. Appl. Physiol. 63:152.PubMedGoogle Scholar
  5. Cheeseman, K.H., and Slater, T.F. 1993, An introduction to free radical biochemistry. Brit. Med. Bulletin 49:481.Google Scholar
  6. Curnutte, J.T. 1993, Chronic granulomatous disease: the solving of a clinical riddle at the molecular level. Clin. Immunol. Immunopathol. 67:S2.PubMedCrossRefGoogle Scholar
  7. Esterbauer, H., Schaur, R.J., and Zollner, H. 1991, Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic. Biol. Med. 11:81.PubMedCrossRefGoogle Scholar
  8. Grisham, M.B. 1992, “Reactive Metabolites of Oxygen and Nitrogen in Biology and Medicine”, R.G. Landes Company, Austin.Google Scholar
  9. Mitchell, D.Y., and Petersen, D.R. 1987, The oxidation of α-β unsaturated aldehydic products of lipid peroxidation by rat liver aldehyde dehydrogenases. Tox. Appl. Pharmacol. 87:403.CrossRefGoogle Scholar
  10. Moslen, M.T. 1992, Protection against free radical-mediated tissue injury, in: “Free Radical Mechanisms of Tissue Injury”, M.T. Moslen and C.V. Smith, eds., CRC Press, Boca Raton.Google Scholar
  11. Oberley, T.D., Oberley, L.W., Slattery, A.F., Launchner, L.J. and Elwell, J.H. 1990, Immunohistochemical localization of antioxidant enzymes in adult Syrian hamster tissues and during kidney development. Am. J. Pathol. 137:199.PubMedGoogle Scholar
  12. Ogihara, T., Miyake, M., Kawamura, N., Tamai, H., Kitagawa, M., and Mino, M. 1989, Tocopherol concentrations of leukocytes in neonates, Ann. N. Y. Acad. Sci. 570:487.CrossRefGoogle Scholar
  13. Smith, R.M., and Curnutte, J.T., 1991, Molecular basis of chronic granulomatous disease. Blood 77:673.PubMedGoogle Scholar
  14. Thelen, M., Dewald, B., and Baggiolini, M. 1993, Neutrophil signal transduction and activation of the respiratory burst. Physiol. Rev. 73:797.PubMedGoogle Scholar
  15. van den Berg, J.J.M., Op den Kamp, J.A.F., Lubin, B.H. and Kuypers, F.A. 1993, Conformational changes in oxidized phospholipids and their preferential hydrolysis by phospholipase A2: a monolayer study. Biochemistry 32:4962.PubMedCrossRefGoogle Scholar
  16. Winrow, V.R., Winyard, P.G., Morris, C.J., and Blake, D.R. 1993, Free radicals in inflammation: Second messengers and mediators of tissue destruction. Brit. Med. Bulletin 49:506.Google Scholar
  17. Yamamoto, S. 1991. “Enzymatic” lipid peroxidation: reactions of mammalian lipoxygenases. Free Radic. Biol. Med. 10:149.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Mary Treinen Moslen
    • 1
  1. 1.Department of PathologyUniversity of Texas Medical BranchGalvestonUSA

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