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One- and Two-Photon Confocal Fluorescence Lifetime Imaging and Its Applications

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Methods in Cellular Imaging

Part of the book series: Methods in Physiology ((METHPHYS))

Abstract

Fluorescence microscopy is a commonly used tool in biological and biophysical research. The great power of fluorescence microscopy lies in the excellent contrast, selectivity, and sensitivity that can be achieved with this technique. The high contrast stems from the shift between the wavelength at which the specimen is excited and the wavelength of the emitted fluorescence light. This shift, the Stokes’ shift, enables the efficient suppression of scattered excitation light by means of simple optical cut-off or bandpass filters. The contrast and sensitivity that can be realized are so high that even fluorescence imaging of single molecules can be accomplished.

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Authors
  1. Hans C. Gerritsen
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  2. Kees de Grauw
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Editor information

Editors and Affiliations

  1. W. M. Keck Center for Cellular Imaging, University of Virginia, Charlottesville, Virginia, USA

    Ammasi Periasamy Ph.D. (Director, Professor of Biology and Biomedical Engineering) (Director, Professor of Biology and Biomedical Engineering)

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© 2001 American Physiological Society

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Gerritsen, H.C., de Grauw, K. (2001). One- and Two-Photon Confocal Fluorescence Lifetime Imaging and Its Applications. In: Periasamy, A. (eds) Methods in Cellular Imaging. Methods in Physiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7513-2_18

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  • DOI: https://doi.org/10.1007/978-1-4614-7513-2_18

  • Publisher Name: Springer, New York, NY

  • Online ISBN: 978-1-4614-7513-2

  • eBook Packages: Springer Book Archive

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