Methods in Cellular Imaging

  • Ammasi Periasamy

Part of the Methods in Physiology book series (METHPHYS)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Basics of Fluorescence, Fluorophores, Microscopy, and Detectors

    1. Front Matter
      Pages 1-1
    2. Keith Berland
      Pages 5-19
    3. Kenneth R. Spring
      Pages 40-52
    4. John J. Lemasters, Ting Qian, Donna R. Trollinger, Barbara J. Muller-Borer, Steven P. Elmore, Wayne E. Cascio
      Pages 66-87
    5. Michael R. Duchen, Jake Jacobson, Julie Keelan, Mart H. Mojet, Olga Vergun
      Pages 88-111
    6. Alan S. Verkman, Lakshmanan Vetrivel, Peter Haggie
      Pages 112-127
  3. Multiphoton Excitation Fluorescence Microscopy

    1. Front Matter
      Pages 143-143
    2. Peter T. C. So, Ki H. Kim, Christof Buehler, Barry R. Masters, Lily Hsu, Chen-Yuan Dong
      Pages 147-161
    3. Vincent P. Wallace, Andrew K. Dunn, Mariah L. Coleno, Bruce J. Tromberg
      Pages 180-199
    4. Mary E. Dickinson, Scott E. Fraser
      Pages 200-215
  4. Fluorescence Resonance Energy Transfer and Lifetime Imaging Microscopy

    1. Front Matter
      Pages 253-256
    2. Brian Herman, Gerald Gordon, Nupam Mahajan, Victoria Centonze
      Pages 257-272
    3. Peter J. Verveer, Anthony Squire, Philippe I. H. Bastiaens
      Pages 273-294
    4. Ammasi Periasamy, Masilamani Elangovan, Horst Wallrabe, Magarida Barroso, James N. Demas, David L. Brautigan et al.
      Pages 295-308
    5. Chen-Yuan Dong, Christof Buehler, Peter T. C. So, Todd French, Enrico Gratton
      Pages 324-340
  5. Other Advanced Methods in Cellular Imaging

  6. Back Matter
    Pages 425-434

About this book


Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.


Cellular Imaging biology biophysics cell cell biology gene gene expression membrane microscopy protein-protein interactions spectroscopy tissue

Editors and affiliations

  • Ammasi Periasamy
    • 1
  1. 1.W. M. Keck Center for Cellular ImagingUniversity of VirginiaCharlottesvilleUSA

Bibliographic information