Abstract
The integration of ultraviolet (UV) and visible (VIS) laser radiation in optical microscopes has led to significant advances in diagnostic methods in life sciences, including three-dimensional (3D) fluorescence imaging by confocal laser scanning microscopy (CLSM) (see Chapter 5), high-resolution fluorescence imaging by scanning near-field optical microscopy (SNOM), and fluorescence lifetime imaging (FLIM) (see Chapters 15–19) with subnanosecond temporal resolution. Further applications of these laser microscopes include intracellular noncontact microsurgery by powerful laser pulses and microphotochemistry, such as UV-induced release of caged compounds.
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König, K. (2001). Cellular Response to Laser Radiation in Fluorescence Microscopes. In: Periasamy, A. (eds) Methods in Cellular Imaging. Methods in Physiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7513-2_14
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DOI: https://doi.org/10.1007/978-1-4614-7513-2_14
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