Abstract
Molecular excitation by two-photon absorption and the subsequent fluorescence have proved to be a useful tool for imaging biological systems using laser-scanning microscopy (Denk et al., 1990). In two-photon fluorescence microscopy (TPM), near-infrared (NIR) light is used to excite transitions of twice the energy of a single photon. One of the main advantages of TPM over conventional fluorescence imaging is that the NIR excitation light penetrates more deeply into tissue than the corresponding one-photon excitation wavelength. This is because factors governing light propagation (i.e., tissue absorption and scattering) are substantially reduced in this spectral region. As a result, TPM offers the possibility of probing relatively thick tissue with submicron resolution.
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Wallace, V.P., Dunn, A.K., Coleno, M.L., Tromberg, B.J. (2001). Two-Photon Microscopy in Highly Scattering Tissue. In: Periasamy, A. (eds) Methods in Cellular Imaging. Methods in Physiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7513-2_11
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DOI: https://doi.org/10.1007/978-1-4614-7513-2_11
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