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Genome-Wide RNAi Screening to Identify Human Host Factors Crucial for Influenza Virus Replication

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RNA Interference from Biology to Therapeutics

Part of the book series: Advances in Delivery Science and Technology ((ADST))

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Abstract

Influenza A virus has developed strategies to exploit and in some cases subvert cellular proteins and pathways to promote its own replication and to suppress antiviral immune responses. Identification of these host factors would expand the number of potential drug targets far beyond the 11 proteins encoded in the viral genome. Recently, several laboratories have set out to provide an insight into the interface between influenza virus and its host by performing genome-wide siRNA silencing screens to characterize these host proteins and to monitor the effects on viral infectivity. Initial hits from each study were used to search databases of protein–protein interactions, allowing prediction of host-cell pathways likely to be involved either in the viral replicative cycle or in the immune response to viral infection. The results of these screens will promote our understanding of influenza virus biology as well as identify potential targets for the rational design of broad-spectrum antiviral drugs such as siRNA and small molecules.

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Acknowledgments

We thank T.F. Meyer for providing constructive comments and K. Holden-Dye for critical reading of the manuscript. AK and KA are supported by European Commission funding under the 7th Framework Programme HEALTH.2010.2.3.3-4 Novel therapeutics against influenza; FP7-INFLUENZA-2010.

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Authors and Affiliations

  1. Max Planck Institute for Infection Biology, Charitéplatz 1, 10117, Berlin, Germany

    Katharina Ahrens & Alexander Karlas

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  1. Katharina Ahrens
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  2. Alexander Karlas
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Correspondence to Alexander Karlas .

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Editors and Affiliations

  1. Interdisciplinary Nanoscience Center, Aarhus University, Ny Munkegade 118, Aarhus, 8000 AA, Denmark

    Kenneth A. Howard

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Ahrens, K., Karlas, A. (2013). Genome-Wide RNAi Screening to Identify Human Host Factors Crucial for Influenza Virus Replication. In: Howard, K. (eds) RNA Interference from Biology to Therapeutics. Advances in Delivery Science and Technology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-4744-3_12

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